Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 159 82.1 2830106 99.3% 2810295 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,682,766 A→G D203G (GAC→GGC)  rstA → DNA‑binding transcriptional regulator RstA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,682,7660AG100.0% 212.3 / NA 57D203G (GAC→GGC) rstADNA‑binding transcriptional regulator RstA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/30);  total (27/30)

ACTGAAATCTCGCTCTCGACAGCTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAAAAACTGTTTATCC  >  NC_000913/1682630‑1682900
                                                                                                                                        |                                                                                                                                      
acTGAAATCTCGCTCTCGACAGCTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGt                                                                                                                                     >  2:42358/1‑140 (MQ=255)
      aTCTAGCTCTCGACAGCTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCGTTGCTGAAAAATTTACGCGGCGTCAGTTATGACGAACTGGATCGTAGCGTGGGCGTGGCTAt                                                                                                                               >  2:862252/1‑140 (MQ=255)
          cGCTCTCGACAGCTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTcg                                                                                                                           >  1:858059/1‑140 (MQ=255)
            ctctCGACAGCTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTcgcg                                                                                                                         >  2:1320100/1‑140 (MQ=255)
               tcGACAGCTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGtt                                                                                                                      <  1:1048338/140‑1 (MQ=255)
                      cTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGaaaa                                                                                                               >  2:1014421/1‑140 (MQ=255)
                                attattGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCg                                                                                                     >  1:56533/1‑140 (MQ=255)
                                  tattGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGAt                                                                                                   <  2:486851/140‑1 (MQ=255)
                                    ttGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATaa                                                                                                 <  1:42358/140‑1 (MQ=255)
                                      gtgGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAAcg                                                                                               <  2:256592/140‑1 (MQ=255)
                                       tgGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAAcgc                                                                                              >  1:1087932/1‑140 (MQ=255)
                                         ggAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAAcgccg                                                                                            <  1:690729/140‑1 (MQ=255)
                                           aaTTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCa                                                                                          <  2:1051226/140‑1 (MQ=255)
                                              tAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGaa                                                                                       >  1:1134376/1‑140 (MQ=255)
                                               aGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAAc                                                                                      >  1:413749/1‑140 (MQ=255)
                                                    cccATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTAt                                                                                 <  1:1306967/140‑1 (MQ=255)
                                                    cccATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTAt                                                                                 <  2:954882/140‑1 (MQ=255)
                                                    cccATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTAt                                                                                 <  1:359072/140‑1 (MQ=255)
                                                    cccATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTAt                                                                                 <  2:744228/140‑1 (MQ=255)
                                                    cccATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTAt                                                                                 <  1:745544/140‑1 (MQ=255)
                                                      cATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCg                                                                               <  2:1351184/140‑1 (MQ=255)
                                                      cATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCg                                                                               <  2:252240/140‑1 (MQ=255)
                                                         gCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCAt                                                                            >  1:448813/1‑140 (MQ=255)
                                                         gCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCAt                                                                            >  1:224985/1‑140 (MQ=255)
                                                         gCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCAt                                                                            >  1:918800/1‑140 (MQ=255)
                                                         gCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCAt                                                                            >  1:134813/1‑140 (MQ=255)
                                                                  aTCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACtgt                                                                   >  2:83716/1‑140 (MQ=255)
                                                                          ccGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACa                                                           <  2:448813/140‑1 (MQ=255)
                                                                             cgATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAg                                                        >  1:421279/1‑140 (MQ=255)
                                                                             cgATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAg                                                        >  1:975811/1‑140 (MQ=255)
                                                                             cgATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAg                                                        >  1:1342055/1‑140 (MQ=255)
                                                                              gATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAgg                                                       <  1:83716/140‑1 (MQ=255)
                                                                              gATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAgg                                                       >  1:1218712/1‑140 (MQ=255)
                                                                                       cTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCtttt                                              >  2:383157/1‑140 (MQ=255)
                                                                                        tGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCttttt                                             <  2:762828/140‑1 (MQ=255)
                                                                                        tGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCttttt                                             <  2:1342055/140‑1 (MQ=255)
                                                                                         gAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTg                                            <  2:1134376/140‑1 (MQ=255)
                                                                                          aaaaaTTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTgc                                           <  1:1166190/140‑1 (MQ=255)
                                                                                               tttACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTc                                      <  2:308262/140‑1 (MQ=255)
                                                                                               tttACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTc                                      <  1:1320100/140‑1 (MQ=255)
                                                                                                ttACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTca                                     <  2:1218712/140‑1 (MQ=255)
                                                                                                   cgcgGCGTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcatgc                                  <  2:594083/140‑1 (MQ=255)
                                                                                                    gcggcgTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcatgca                                 <  1:1014421/140‑1 (MQ=255)
                                                                                                     cggcgTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcatgcat                                <  2:134813/140‑1 (MQ=255)
                                                                                                      ggcgTCAGTTATGACGGACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTcatgcatg                               <  2:975811/140‑1 (MQ=255)
                                                                                                                      gACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGaaa               >  2:957696/1‑140 (MQ=255)
                                                                                                                      gACTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGaaa               >  1:846115/1‑140 (MQ=255)
                                                                                                                       aCTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGaaaa              <  2:227373/140‑1 (MQ=255)
                                                                                                                       aCTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGaaaa              >  1:1057575/1‑140 (MQ=255)
                                                                                                                        cTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGaaaaa             >  1:169318/1‑140 (MQ=255)
                                                                                                                        cTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGaaaaa             >  2:494932/1‑140 (MQ=255)
                                                                                                                        cTGGATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGaaaaa             >  1:1116660/1‑140 (MQ=255)
                                                                                                                           gATCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGCGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAAAAACt          >  2:407755/1‑140 (MQ=255)
                                                                                                                            aTCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAAAAACTg         >  2:7234/1‑140 (MQ=255)
                                                                                                                             tCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAAAAACTGt        <  1:494932/140‑1 (MQ=255)
                                                                                                                             tCGTAGCGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAAAAACTGt        <  2:1057575/140‑1 (MQ=255)
                                                                                                                                   cGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAAAAACTGTTTATcc  >  1:82480/1‑140 (MQ=255)
                                                                                                                                   cGTGGGCGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAAAAACTGTTTATcc  <  2:846115/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ACTGAAATCTCGCTCTCGACAGCTGATTTCGAATTATTGTGGGAATTAGCTACCCATGCCGGGCAAATCATGGACCGCGATGCATTGCTGAAAAATTTACGCGGCGTCAGTTATGACGGACTGGATCGTAGCGTGGACGTGGCTATTTCGCGGTTAAGAAAAAAACTGCTCGATAACGCCGCAGAACCTTATCGCATTAAAACTGTGCGTAACAAAGGCTATCTTTTTGCGCCTCATGCATGGGAATAAGCGATGAAAAAACTGTTTATCC  >  NC_000913/1682630‑1682900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A