Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,714,949	A→G	V191V (GTA→GTG)	gstA →	glutathione S‑transferase GstA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,714,949	0	A	G	100.0%	176.0 / NA	47	V191V (GTA→GTG)	gstA	glutathione S‑transferase GstA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/23); total (24/23)

CGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCAC  >  NC_000913/1714817‑1715085

cGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGAcg                                                                                                                                   >  1:1088204/1‑140 (MQ=255)

  ggCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGAcgcg                                                                                                                                 <  2:165233/140‑1 (MQ=255)

  ggCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGAcgcg                                                                                                                                 <  2:524235/140‑1 (MQ=255)

  ggCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGAcgcg                                                                                                                                 <  2:54077/140‑1 (MQ=255)

      aaGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGt                                                                                                                             <  1:892632/140‑1 (MQ=255)

            tACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCgg                                                                                                                       <  2:618839/140‑1 (MQ=255)

            tACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCgg                                                                                                                       <  1:1363496/140‑1 (MQ=255)

                aTTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAgg                                                                                                                   <  2:81223/140‑1 (MQ=255)

                  tGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCt                                                                                                                 <  1:567448/140‑1 (MQ=255)

                   gCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCtt                                                                                                                >  1:2123/1‑140 (MQ=255)

                    cTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTa                                                                                                               <  1:1111951/140‑1 (MQ=255)

                    cTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTa                                                                                                               <  2:1372696/140‑1 (MQ=255)

                    cTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTa                                                                                                               <  1:692075/140‑1 (MQ=255)

                    cTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTa                                                                                                               <  2:532792/140‑1 (MQ=255)

                              tCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGc                                                                                                     >  1:1087230/1‑140 (MQ=255)

                                tGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCa                                                                                                   <  2:1088204/140‑1 (MQ=255)

                                 gTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCAt                                                                                                  <  1:176057/140‑1 (MQ=255)

                                    tACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCgg                                                                                               >  2:704624/1‑140 (MQ=255)

                                       ggTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGccc                                                                                            <  2:1294664/140‑1 (MQ=255)

                                            tGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCAt                                                                                                  >  1:1410222/1‑129 (MQ=255)

                                            tGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCAt                                                                                                  <  2:1410222/129‑1 (MQ=255)

                                            tGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAgg                                                                                       >  1:34062/1‑140 (MQ=255)

                                            tGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAgg                                                                                       >  2:1087084/1‑140 (MQ=255)

                                                   ggCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTtgt                                                                                >  2:397190/1‑140 (MQ=255)

                                                   ggCATACGCGGTGAAACTGAATCTGGAA‑GGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTtgtg                                                                               >  2:733525/1‑140 (MQ=255)

                                                       tACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGc                                                                            <  1:133666/140‑1 (MQ=255)

                                                             gTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAAt                                                                      >  1:1139766/1‑140 (MQ=255)

                                                             gTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAAt                                                                      >  1:283668/1‑140 (MQ=255)

                                                                                ggTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTttg                                                   >  1:780550/1‑140 (MQ=255)

                                                                                  ttAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTttgtt                                                 >  2:293224/1‑140 (MQ=255)

                                                                                   tAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTttgttg                                                <  1:1087084/140‑1 (MQ=255)

                                                                                    agagCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTgc                                               >  2:575475/1‑140 (MQ=255)

                                                                                      agCACATTGGAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTgcgc                                             >  2:757846/1‑140 (MQ=255)

                                                                                           aTTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAg                                        >  2:340432/1‑140 (MQ=255)

                                                                                           aTTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAg                                        >  1:328900/1‑140 (MQ=255)

                                                                                            ttGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGt                                       >  1:677197/1‑140 (MQ=255)

                                                                                                  caTTTATGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGtt                                 <  2:780550/140‑1 (MQ=255)

                                                                                                        tGCAACGTATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGtt                           <  2:634940/140‑1 (MQ=255)

                                                                                                               tATGGCTGAACGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAAt                    >  2:262611/1‑140 (MQ=255)

                                                                                                                       aaCGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCt            >  1:868579/1‑140 (MQ=255)

                                                                                                                       aaCGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCt            >  1:736205/1‑140 (MQ=255)

                                                                                                                       aaCGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCt            >  1:577719/1‑140 (MQ=255)

                                                                                                                       aaCGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCt            >  1:1065932/1‑140 (MQ=255)

                                                                                                                         cGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGa          <  2:34062/140‑1 (MQ=255)

                                                                                                                         cGTCCGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGa          <  2:1065932/140‑1 (MQ=255)

                                                                                                                             cGGAAGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGagcag      >  1:150639/1‑140 (MQ=255)

                                                                                                                                 aGTGCAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGccac  <  2:1310648/140‑1 (MQ=255)

CGGGCAAAGATTTACAATTGCTGATGCCTATCTGTTTACGGTTCTGCGCTGGGCATACGCGGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCAC  >  NC_000913/1714817‑1715085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A