Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,755,350	T→C	intergenic (‑209/‑348)	fumD ← / → pykF	fumarase D/pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,350	0	T	C	100.0%	272.0 / NA	75	intergenic (‑209/‑348)	fumD/pykF	fumarase D/pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (38/37); total (38/37)

AGACAAATAACGCGATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAT  >  NC_000913/1755216‑1755483

aGACAAATAACGCGATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCt                                                                                                                                  <  2:409728/140‑1 (MQ=255)

      aTAACGCGATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTccc                                                                                                                            >  2:754291/1‑140 (MQ=255)

        aaCGCGATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTg                                                                                                                          <  1:29637/140‑1 (MQ=255)

         aCGCGATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTgg                                                                                                                         <  1:315561/140‑1 (MQ=255)

          cgcgATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGa                                                                                                                        >  2:1405656/1‑140 (MQ=255)

          cgcgATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGa                                                                                                                        >  2:1101473/1‑140 (MQ=255)

            cgATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTccc                                                                                                                            >  2:957334/1‑134 (MQ=255)

            cgATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTccc                                                                                                                            <  1:957334/134‑1 (MQ=255)

               tAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGtt                                                                                                                   <  2:277070/140‑1 (MQ=255)

                  attattTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTaaa                                                                                                                <  2:1075480/140‑1 (MQ=255)

                         tAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGa                                                                                                         <  1:547114/140‑1 (MQ=255)

                         tAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGa                                                                                                         >  1:792489/1‑140 (MQ=255)

                             tGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAAc                                                                                                     <  1:1251647/140‑1 (MQ=255)

                              gTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACa                                                                                                    <  1:1401659/140‑1 (MQ=255)

                              gTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACa                                                                                                    >  2:371664/1‑140 (MQ=255)

                                aGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAAt                                                                                                  >  1:147582/1‑140 (MQ=255)

                                 gCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAAtt                                                                                                 <  1:842268/140‑1 (MQ=255)

                                   aaTTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTa                                                                                               <  1:912029/140‑1 (MQ=255)

                                   aaTTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTa                                                                                               <  1:703078/140‑1 (MQ=255)

                                     ttGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTAtt                                                                                             <  2:525565/140‑1 (MQ=255)

                                     ttGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTAtt                                                                                             <  2:702269/140‑1 (MQ=255)

                                       gAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGt                                                                                           >  2:1396630/1‑140 (MQ=255)

                                         gCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCACCCTTTTCCCTGGAACGTTAAATCTTTGAt                                                                                                        <  2:1103905/125‑1 (MQ=255)

                                         gCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGAt                                                                                                        >  1:1103905/1‑125 (MQ=255)

                                                       ataCACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTatat                                                                           >  2:798919/1‑140 (MQ=255)

                                                       ataCACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTatat                                                                           >  2:1129116/1‑140 (MQ=255)

                                                       ataCACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTatat                                                                           >  2:241969/1‑140 (MQ=255)

                                                         acacCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATAttt                                                                         >  1:1288083/1‑140 (MQ=255)

                                                         acacCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATAttt                                                                         >  2:817042/1‑140 (MQ=255)

                                                         acacCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATAttt                                                                         >  2:534084/1‑140 (MQ=255)

                                                            ccGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATAtttttt                                                                      >  1:1165961/1‑140 (MQ=255)

                                                            ccGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATAtttttt                                                                      >  2:751994/1‑140 (MQ=255)

                                                            ccGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATAtttttt                                                                      >  2:647913/1‑140 (MQ=255)

                                                             cGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTg                                                                     >  2:166735/1‑140 (MQ=255)

                                                             cGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTg                                                                     <  1:1396630/140‑1 (MQ=255)

                                                             cGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTg                                                                     >  1:959512/1‑140 (MQ=255)

                                                              ggATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGa                                                                    >  1:718914/1‑140 (MQ=255)

                                                               gATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGaa                                                                   >  2:175971/1‑140 (MQ=255)

                                                               gATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGaa                                                                   >  1:847106/1‑140 (MQ=255)

                                                               gATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGaa                                                                   >  1:192462/1‑140 (MQ=255)

                                                               gATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGaa                                                                   >  2:612323/1‑140 (MQ=255)

                                                                aTGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGaaa                                                                  <  2:847106/140‑1 (MQ=255)

                                                                     cTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtg                                                             <  1:1101473/140‑1 (MQ=255)

                                                                       ttCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtgtt                                                           <  1:754291/140‑1 (MQ=255)

                                                                              aTCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCtgtttttgttt                                                    >  2:891520/1‑140 (MQ=255)

                                                                                   cacaCTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCtt                                               <  1:166735/140‑1 (MQ=255)

                                                                                   cacaCTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCtt                                               >  2:1018859/1‑140 (MQ=255)

                                                                                          aCAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGGtt                                        >  2:226601/1‑140 (MQ=255)

                                                                                          aCAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGAtt                                        >  2:775412/1‑140 (MQ=255)

                                                                                          aCAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGAtt                                        >  2:1349348/1‑140 (MQ=255)

                                                                                          aCAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGAtt                                        >  1:949136/1‑140 (MQ=255)

                                                                                          aCAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGAtt                                        <  1:371664/140‑1 (MQ=255)

                                                                                          aCAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGAtt                                        >  1:500803/1‑140 (MQ=255)

                                                                                          aCAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGAt                                         >  1:542611/1‑139 (MQ=255)

                                                                                          aCAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGAt                                         <  2:542611/139‑1 (MQ=255)

                                                                                              cTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAAtt                                    <  1:32738/140‑1 (MQ=255)

                                                                                               ttCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAAttt                                   <  1:34849/140‑1 (MQ=255)

                                                                                               ttCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAAttt                                   <  2:959512/140‑1 (MQ=255)

                                                                                                tCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTc                                  <  1:1405656/140‑1 (MQ=255)

                                                                                                tCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTc                                  <  2:949136/140‑1 (MQ=255)

                                                                                                tCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTc                                  >  1:404058/1‑140 (MQ=255)

                                                                                                 cGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCa                                 <  1:534084/140‑1 (MQ=255)

                                                                                                    cACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCg                              >  2:1347833/1‑140 (MQ=255)

                                                                                                             ttGCGCAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcg                     <  1:304580/140‑1 (MQ=255)

                                                                                                                cgcAAACAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATgcgcgc                  >  1:1179702/1‑140 (MQ=255)

                                                                                                                     aCAGTGAAGTTTTTGCGCAGCCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAAtt             <  1:825211/140‑1 (MQ=255)

                                                                                                                     aCAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAAtt             <  2:500803/140‑1 (MQ=255)

                                                                                                                     aCAGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAAtt             >  2:1164846/1‑140 (MQ=255)

                                                                                                                       aGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGa           <  1:1349348/140‑1 (MQ=255)

                                                                                                                       aGTGAAGTTTTTGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGa           <  1:775412/140‑1 (MQ=255)

                                                                                                                              tttttGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGa    <  1:817042/140‑1 (MQ=255)

                                                                                                                                tttGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGa    <  1:1320263/138‑1 (MQ=255)

                                                                                                                                tttGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGa    >  2:1320263/1‑138 (MQ=255)

                                                                                                                                tttGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAt  <  1:1347833/140‑1 (MQ=255)

                                                                                                                                tttGCGCAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAt  <  2:1179702/140‑1 (MQ=255)

AGACAAATAACGCGATAAATTATTTTAACTGTAGCAATTGAGCGATGATATATTTATACACCGGATGAACTTTCACTTATCCTCACACTGACAACTTCGGCACCAGACGTTGCGCAAACAGTGAAGTTTTTGCGTAACCTTTTCCCTGGAACGTTAAATCTTTGATAACAATTTATTGTCTAACAAGTTGTATATTTTTTGAAACGCTGTTTTTGTTTTCCTTTTGGATTAATTTCAGCGTATAATGCGCGCCAATTGACTCTTGAAT  >  NC_000913/1755216‑1755483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A