Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 159 82.1 2830106 99.3% 2810295 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,148,330 T→C G219G (GGT→GGC)  yegD → HSP70 family protein YegD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,148,3300TC100.0% 65.7 / NA 19G219G (GGT→GGC) yegDHSP70 family protein YegD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/17);  total (2/17)

ACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCT  >  NC_000913/2148204‑2148464
                                                                                                                               |                                                                                                                                      
aCGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGtt                                                                                                                           <  2:251523/140‑1 (MQ=255)
 cgcgcgGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTc                                                                                                                          >  1:1097533/1‑140 (MQ=255)
   ggcggggAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCAt                                                                                                                        <  2:558880/139‑1 (MQ=255)
   cgcgGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGGACGactgactggttct                                                                                                                        >  2:1193324/1‑135 (MQ=255)
   cgcgGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACgac                                                                                                                                   >  1:1318811/1‑129 (MQ=255)
   cgcgGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACgac                                                                                                                                   <  2:1318811/129‑1 (MQ=255)
   cgcgGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCAt                                                                                                                        <  2:918949/140‑1 (MQ=255)
           aGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGGGCGACCGACTGGTCTTTTctgctg                                                                                                                >  2:6374/1‑140 (MQ=255)
              gTGCCGGATTCAGGGACGT‑GTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATgg                                                                                                            <  2:1155403/140‑1 (MQ=255)
                 ccGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATgggg                                                                                                          <  2:58098/140‑1 (MQ=255)
                    gATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCg                                                                                                       <  1:1397596/140‑1 (MQ=255)
                              cGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGtt                                                                                             <  1:606368/140‑1 (MQ=255)
                               gTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGGCCGACTGACTGGTTATTTCTGCTGGTGGGGGCGCAgggggggtt                                                                                            >  2:114224/1‑135 (MQ=255)
                                       ccAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCCCCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCg                                                                                    <  2:486575/140‑1 (MQ=255)
                                         aGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGGACGACTGACTGTTTATTTTTGCTGGTGGGGGCGCCGGGGGGGTTGCGtctccgt                                                                                  >  1:33369/1‑137 (MQ=255)
                                           tACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACCGACTGTTTCTTTCTTCTGGTGGGGGCGCCGTGGGGGTTGCGGCTCCATCg                                                                                >  1:686967/1‑140 (MQ=255)
                                                     tCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGCTGGGCCACCGGAC‑GATTATTGCTGCTTATTGGGGCGCGGGGGGGGGTGCGGCTCGATCGTGAagccagc                                                                      >  2:504330/1‑140 (MQ=255)
                                                     tCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGGACGACCGACTGTTTATTTCTGCTGGTGGGGGCGCCGGGGGGGTTGCGTCTCGATCGTGGaggcagc                                                                      >  2:837862/1‑140 (MQ=255)
                                                        cgGCTGGGGTGGAATACGAAGGCCCCTTGCAGGAAGGAAAACCGGTGCTGGTGGTTGATAACGGCGG‑GGGGACCACTGACCGTTCATTGCTGCTGGTGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGGctg                                                                   <  2:28844/140‑1 (MQ=255)
                                                        cgGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCctg                                                                   <  1:837862/140‑1 (MQ=255)
                                                        cgGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCctg                                                                   <  1:381081/140‑1 (MQ=255)
                                                        cgGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCctg                                                                   <  1:36708/140‑1 (MQ=255)
                                                              ggctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGt                                                             <  2:736630/140‑1 (MQ=255)
                                                              ggctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGt                                                             <  2:922947/140‑1 (MQ=255)
                                                                  ggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGAGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTcaca                                                         <  2:1097533/140‑1 (MQ=255)
                                                                       aCGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGCCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGt                                                    <  1:480424/140‑1 (MQ=255)
                                                                             ccACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGGACGACTGACTGTTTATTGCTGCTGGTGGGGGCGCCGTGGGGGTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGGCACAGTGGGTGGCgg                                              >  2:637522/1‑139 (MQ=255)
                                                                              cACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGGACGACTGACTGTTCATTGCTGCTGATGGGGGCGCAGGGGGGGTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGGCACAGGGGGTGCCgga                                             >  2:595740/1‑138 (MQ=255)
                                                                                                             gtggATATCGGCGG‑TGGCACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGc              <  1:114224/140‑1 (MQ=255)
                                                                                                                         gg‑tggcACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCt  <  1:504330/140‑1 (MQ=255)
                                                                                                                               |                                                                                                                                      
ACGCGCGGCGAAGCGTGCCGGATTCAGGGACGTGGTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGG‑TGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGGGTCACAGTGGTTGCCGTATTGGCGGTAACGATCTGGATATCGCGCTGGCGTTTAAAAACCT  >  NC_000913/2148204‑2148464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A