Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,020,551	A→T	Q4H (CAA→CAT)	ygfM →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,020,551	0	A	T	100.0%	176.8 / NA	50	Q4H (CAA→CAT)	ygfM	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (26/24); total (26/24)

ATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTA  >  NC_000913/3020416‑3020686

aTTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATtttt                                                                                                                                     >  2:957135/1‑140 (MQ=255)

aTTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATtttt                                                                                                                                     <  1:790354/140‑1 (MQ=255)

 ttGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAtttttt                                                                                                                                    <  2:168056/140‑1 (MQ=255)

 ttGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAtttttt                                                                                                                                    <  1:811251/140‑1 (MQ=255)

            tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGAct                                                                                                                         <  2:1222618/140‑1 (MQ=255)

                    caAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGaa                                                                                                                 <  2:204421/140‑1 (MQ=255)

                    caAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGaa                                                                                                                 <  1:368589/140‑1 (MQ=255)

                      aGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACa                                                                                                               >  2:808935/1‑140 (MQ=255)

                       gCCAGAAAAGCCGCTGCCAGTGTGTGGCGGCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGGTAAATAAGGAACGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGACCAg                                                                                                              >  2:276405/1‑140 (MQ=255)

                       gCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAg                                                                                                              >  2:725929/1‑140 (MQ=255)

                       gCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAg                                                                                                              >  2:131854/1‑140 (MQ=255)

                        ccAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAgg                                                                                                             <  1:1117156/140‑1 (MQ=255)

                           gAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGgcgc                                                                                                          >  2:1210364/1‑140 (MQ=255)

                                 ccGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAAc                                                                                                    >  2:1058655/1‑140 (MQ=255)

                                 ccGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGGTAAATATGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAAc                                                                                                    >  2:833925/1‑140 (MQ=255)

                                      gCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACATATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGACCATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAg                                                                                               <  2:1320190/140‑1 (MQ=255)

                                      gCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAg                                                                                               <  1:1058655/140‑1 (MQ=255)

                                         aGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAgcgc                                                                                            <  1:725929/140‑1 (MQ=255)

                                               tgGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTAc                                                                                      <  1:131854/140‑1 (MQ=255)

                                               tgGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTAc                                                                                      <  1:7367/140‑1 (MQ=255)

                                                  cGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAg                                                                                   >  2:313535/1‑140 (MQ=255)

                                                    tCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGa                                                                                 >  2:490068/1‑140 (MQ=255)

                                                     cGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGAt                                                                                >  2:464648/1‑140 (MQ=255)

                                                                  tGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGt                                                                   <  2:591449/140‑1 (MQ=255)

                                                                              cAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTTAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgca                                                       >  2:743543/1‑140 (MQ=255)

                                                                              cAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgca                                                       >  2:482585/1‑140 (MQ=255)

                                                                              cAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgca                                                       >  1:222788/1‑140 (MQ=255)

                                                                                 aTGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGgcagca                                                    <  1:490068/140‑1 (MQ=255)

                                                                                  tGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGACGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCaa                                                   >  2:871984/1‑140 (MQ=255)

                                                                                   gCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCaaa                                                  >  1:60052/1‑140 (MQ=255)

                                                                                    cccTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAAc                                                 >  2:390083/1‑140 (MQ=255)

                                                                                     ccTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                                                <  1:482585/140‑1 (MQ=255)

                                                                                     ccTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                                                <  1:1395035/140‑1 (MQ=255)

                                                                                      ctctTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                                                >  2:1331591/1‑139 (MQ=255)

                                                                                      ctctTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACt                                                <  1:1331591/139‑1 (MQ=255)

                                                                                         ttCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCAGGGGCAGCAAACTCAAt                                            <  1:218552/140‑1 (MQ=255)

                                                                                          tCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATg                                           <  1:313535/140‑1 (MQ=255)

                                                                                          tCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATg                                           <  1:743543/140‑1 (MQ=255)

                                                                                           cccGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGc                                          <  1:390083/140‑1 (MQ=255)

                                                                                                 gggAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACAcc                                    <  1:464648/140‑1 (MQ=255)

                                                                                                       agGGTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCg                              <  2:60052/140‑1 (MQ=255)

                                                                                                         ggTTAAATAAGGAAAGAACATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTCCCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTa                            <  2:1289533/140‑1 (MQ=255)

                                                                                                          gTTAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTAc                           >  2:1337010/1‑140 (MQ=255)

                                                                                                            tAAATAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCg                         >  2:828320/1‑140 (MQ=255)

                                                                                                              aaTAAGGAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGAt                       >  2:332707/1‑140 (MQ=255)

                                                                                                                   ggAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATaaaaa                  >  2:421147/1‑140 (MQ=255)

                                                                                                                   ggAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATaaaaa                  >  1:995879/1‑140 (MQ=255)

                                                                                                                   ggAAAGAAGATGACTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATaaaaa                  >  2:538047/1‑140 (MQ=255)

                                                                                                                    gAAAGAAGATGATTGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAg                 >  1:60848/1‑140 (MQ=255)

                                                                                                                                 tGAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCt    >  1:533550/1‑140 (MQ=255)

                                                                                                                                  gAACATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCtt   <  1:828320/140‑1 (MQ=255)

                                                                                                                                   aaCATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTa  <  1:833925/140‑1 (MQ=255)

                                                                                                                                   aaCATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTa  <  1:1210364/140‑1 (MQ=255)

                                                                                                                                   aaCATTTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTa  >  2:1176753/1‑140 (MQ=255)

ATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAGGCCCGACTCTGTCGAACAGGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTGGTTCGCCGGGGGCAGCAAACTCAATGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTA  >  NC_000913/3020416‑3020686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A