Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,163,684	A→G	intergenic (‑203/+31)	plsC ← / ← parC	1‑acylglycerol‑3‑phosphate O‑acyltransferase/DNA topoisomerase IV subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,163,684	0	A	G	97.7%	310.4 / ‑2.6	86	intergenic (‑203/+31)	plsC/parC	1‑acylglycerol‑3‑phosphate O‑acyltransferase/DNA topoisomerase IV subunit A
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (38/46); total (38/48)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

AAAGCCGCTCTCTGGAAAAGCGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCA  >  NC_000913/3163548‑3163820

aaaGCCGCTCTCTGGAAAAGCGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCg                                                                                                                                       >  2:596038/1‑140 (MQ=255)

     cGCTCTCTGGAAAAGCGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGggagg                                                                                                                                  >  2:830626/1‑140 (MQ=255)

     cGCTCTCTGGAAAAGCGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGggagg                                                                                                                                  >  1:789589/1‑140 (MQ=255)

     cGCTCTCTGGAAAAGCGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGggagg                                                                                                                                  >  1:705414/1‑140 (MQ=255)

     cGCTCTCTGGAAAAGCGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGggagg                                                                                                                                  >  1:220307/1‑140 (MQ=255)

       ctctctGGAAAAGCGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGaa                                                                                                                                >  1:957646/1‑140 (MQ=255)

       ctctctGGAAAAGCGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGaa                                                                                                                                >  1:722624/1‑140 (MQ=255)

       ctctctGGAAAAGCGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGaa                                                                                                                                >  2:361341/1‑140 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   >  2:996777/1‑140 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   <  1:946728/140‑1 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   <  1:886846/140‑1 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   >  2:100899/1‑140 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   <  2:1071162/140‑1 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   <  2:40455/140‑1 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   <  1:361341/140‑1 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   <  1:1282778/140‑1 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   <  1:1074003/140‑1 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   <  2:705414/140‑1 (MQ=255)

                    cGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTccc                                                                                                                   >  1:1091798/1‑140 (MQ=255)

                      tagtGTTAATCGCAACGCATGAATAAACAACGGTTGCAAGGGAATTTTTATCGTTTCCGGGGGTTGAAGCCGCTGCCGGATGACGACTTAACGTTTCAGCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCg                                                                                                                 <  2:449422/137‑1 (MQ=255)

                      tATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCg                                                                                                                 <  2:1237966/140‑1 (MQ=255)

                      tATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCg                                                                                                                 <  1:596038/140‑1 (MQ=255)

                      tATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCg                                                                                                                 <  1:423155/140‑1 (MQ=255)

                      tATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCg                                                                                                                 <  1:423168/140‑1 (MQ=255)

                           ttAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCata                                                                                                            <  1:1338829/140‑1 (MQ=255)

                            tAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCatat                                                                                                           <  1:1295873/140‑1 (MQ=255)

                              aTCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTa                                                                                                         >  1:493/1‑140 (MQ=255)

                              aTCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTa                                                                                                         >  1:1352341/1‑140 (MQ=255)

                              aTCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTa                                                                                                         >  2:540281/1‑140 (MQ=255)

                                  cAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCg                                                                                                                 >  2:756675/1‑128 (MQ=255)

                                  cAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCg                                                                                                                 <  1:756675/128‑1 (MQ=255)

                                  cAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCCGCATATTActct                                                                                                     >  2:48970/1‑140 (MQ=255)

                                   aaCGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCtt                                                                                                    >  2:1378083/1‑140 (MQ=255)

                                     cGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTActc                                                                                                      >  2:355495/1‑136 (MQ=255)

                                     cGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTActc                                                                                                      <  1:355495/136‑1 (MQ=255)

                                     cGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCg                                                                                                  >  1:570648/1‑140 (MQ=255)

                                     cGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGC‑GGCGGGGGGGAAAAACCCCCCCCCCCGCATATTAATCTTccc                                                                                                 >  1:382005/1‑138 (MQ=255)

                                            aTAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCAc                                                                                           <  2:1352341/140‑1 (MQ=255)

                                             tAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCAcc                                                                                          <  2:676455/140‑1 (MQ=255)

                                                  aacGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCgctgc                                                                                     >  1:224356/1‑140 (MQ=255)

                                                    cGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCAc                                                                                           <  2:273825/132‑1 (MQ=255)

                                                    cGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCAc                                                                                           >  1:273825/1‑132 (MQ=255)

                                                    cGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGctgctg                                                                                   >  1:83139/1‑140 (MQ=255)

                                                    cGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGctgctg                                                                                   >  1:1397532/1‑140 (MQ=255)

                                                     ggTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGGGGGAACAGCGCCCTCCCCGGCATATTACTCTTCCCTATCACCGCTGCTgg                                                                                  >  2:999466/1‑140 (MQ=255)

                                                     ggTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTgg                                                                                  >  1:511281/1‑140 (MQ=255)

                                                      gTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGc                                                                                 <  1:996777/140‑1 (MQ=255)

                                                      gTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGc                                                                                 <  1:594153/140‑1 (MQ=255)

                                                      gTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGc                                                                                 <  2:679623/140‑1 (MQ=255)

                                                      gTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGc                                                                                 <  1:1231649/140‑1 (MQ=255)

                                                      gTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGc                                                                                 <  1:772032/140‑1 (MQ=255)

                                                         tCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACg                                                                              <  1:540281/140‑1 (MQ=255)

                                                                   ttttATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGagag                                                                    <  2:224356/140‑1 (MQ=255)

                                                                    tttaatgTTTCCGGGGGCTGAATCCGCCGCCGGATGACGACCTAACGTTACAACCGGCGTTCCTTTCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTTCTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGt                                                                   <  2:1054497/134‑1 (MQ=255)

                                                                    tttATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGt                                                                   <  1:730042/140‑1 (MQ=255)

                                                                    tttATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGt                                                                   <  2:624016/140‑1 (MQ=255)

                                                                    tttATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGt                                                                   <  2:570648/140‑1 (MQ=255)

                                                                     ttATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTc                                                                  <  2:722624/140‑1 (MQ=255)

                                                                     ttATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTc                                                                  <  1:1287487/140‑1 (MQ=255)

                                                                        tCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGAt                                                               <  2:789589/140‑1 (MQ=255)

                                                                                   ggTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACAcgat                                                    <  1:100899/140‑1 (MQ=255)

                                                                                       gAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACAcgatcgat                                                <  2:511281/140‑1 (MQ=255)

                                                                                       gAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACAcgatcgat                                                <  1:830626/140‑1 (MQ=255)

                                                                                       gAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACAcgatcgat                                                <  2:1397532/140‑1 (MQ=255)

                                                                                         aTACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATAc                                              >  2:45873/1‑140 (MQ=255)

                                                                                          tACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACg                                             >  2:517248/1‑140 (MQ=255)

                                                                                            cGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACAcgat                                                    >  2:363040/1‑131 (MQ=255)

                                                                                            cGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACAcgat                                                    <  1:363040/131‑1 (MQ=255)

                                                                                            cGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCt                                           >  2:774477/1‑140 (MQ=255)

                                                                                               tGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGGCGATCTCAACACGATCGATACGCTGCa                                        >  2:1037345/1‑140 (MQ=255)

                                                                                                 ccGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCaaa                                      >  2:243798/1‑140 (MQ=255)

                                                                                                 ccGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCaaa                                      <  1:679105/140‑1 (MQ=255)

                                                                                                 ccGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCaaa                                      <  1:12874/140‑1 (MQ=255)

                                                                                                      tGACGACTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACcgcg                                 <  2:1351462/140‑1 (MQ=255)

                                                                                                       gacgacTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCgcgc                                >  2:828989/1‑140 (MQ=255)

                                                                                                        acgacTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCa                               <  1:517248/140‑1 (MQ=255)

                                                                                                           acTTAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCa                            >  2:1190018/1‑140 (MQ=255)

                                                                                                              tAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACg                         <  2:268024/140‑1 (MQ=255)

                                                                                                              tAACGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACg                         >  2:1113761/1‑140 (MQ=255)

                                                                                                               aaCGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGt                        <  1:1378083/140‑1 (MQ=255)

                                                                                                               aaCGTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGt                        >  1:518171/1‑140 (MQ=255)

                                                                                                                  gTTTCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTAcc                     <  2:220307/140‑1 (MQ=255)

                                                                                                                    ttCATCCGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGAACGATACGCTGCAAACCGCGCATCAACGTACcgc                   >  1:1012751/1‑140 (MQ=255)

                                                                                                                         ccGGCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGt              <  1:1113761/140‑1 (MQ=255)

                                                                                                                           ggCGTTCCTTGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTcc            >  1:551027/1‑140 (MQ=255)

                                                                                                                                   ttGCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGc    <  2:1091798/140‑1 (MQ=255)

                                                                                                                                     gCAGGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCa  <  1:1403544/140‑1 (MQ=255)

AAAGCCGCTCTCTGGAAAAGCGTATTGTTAATCGCAACGCATGAATAAACAACGGTTTCAAGGGAATTTTTATCGTTTCCGGGGGTTGAATACGCTGCCGGATGACGACTTAACGTTTCATCCGGCGTTCCTTGCAAGCGGGAGGAAACAGCGCCCTCCCCGGCATATTACTCTTCGCTATCACCGCTGCTGGCACGGCGAGGAGAGTCGATCTCAACACGATCGATACGCTGCAAACCGCGCATCAACGTACCGCGGCGTCCACGTTCGCCA  >  NC_000913/3163548‑3163820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A