Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,233,352	G→A	R563H (CGC→CAC)	fadH →	2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,233,352	0	G	A	100.0%	355.9 / NA	100	R563H (CGC→CAC)	fadH	2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (60/40); total (60/40)

Only 100 of 101 total aligned reads displayed.

GGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATG  >  NC_000913/3233216‑3233483

ggCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACaa                                                                                                                                  >  2:77464/1‑140 (MQ=255)

 gCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACaaa                                                                                                                                 >  1:861831/1‑140 (MQ=255)

      tCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAg                                                                                                                            <  1:537557/140‑1 (MQ=255)

      tCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAg                                                                                                                            <  2:208770/140‑1 (MQ=255)

         accaGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCaa                                                                                                                         <  1:498265/140‑1 (MQ=255)

           cagccagAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAAc                                                                                                                       <  2:303688/140‑1 (MQ=255)

           cagccagAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAAc                                                                                                                       <  1:216870/140‑1 (MQ=255)

                 gAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGAc                                                                                                                 <  1:1180816/140‑1 (MQ=255)

                       cGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGt                                                                                                           >  2:960320/1‑140 (MQ=255)

                        gCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGa                                                                                                                  >  2:966385/1‑132 (MQ=255)

                        gCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGa                                                                                                                  <  1:966385/132‑1 (MQ=255)

                            ggTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGc                                                                                                      >  2:940709/1‑140 (MQ=255)

                              ttCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCaa                                                                                                    <  2:298265/140‑1 (MQ=255)

                                      tgaatgGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCg                                                                                            >  2:132565/1‑140 (MQ=255)

                                        aatgGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGc                                                                                          >  1:219720/1‑140 (MQ=255)

                                        aatgGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGc                                                                                          >  1:258315/1‑140 (MQ=255)

                                        aatgGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGc                                                                                          >  2:1193946/1‑140 (MQ=255)

                                        aatgGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGc                                                                                          >  1:737828/1‑140 (MQ=255)

                                             gggATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGAt                                                                                     >  2:250344/1‑140 (MQ=255)

                                             gggATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGAt                                                                                     >  2:848193/1‑140 (MQ=255)

                                             gggATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGAt                                                                                     >  1:90651/1‑140 (MQ=255)

                                             gggATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGAt                                                                                     >  2:874729/1‑140 (MQ=255)

                                                aTCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAGCCACCGGCTGGATCCa                                                                                  <  2:817956/140‑1 (MQ=255)

                                                aTCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCa                                                                                  <  1:548601/140‑1 (MQ=255)

                                                  cGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGAt                                                                                     <  2:1086267/135‑1 (MQ=255)

                                                  cGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGAt                                                                                     >  1:1086267/1‑135 (MQ=255)

                                                  cGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATc                                                                                >  1:102098/1‑140 (MQ=255)

                                                  cGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATc                                                                                >  2:241627/1‑140 (MQ=255)

                                                       gTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGcacc                                                                           >  2:797771/1‑140 (MQ=255)

                                                           ccTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCAccc                                                                       >  2:282030/1‑140 (MQ=255)

                                                           ccTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCAccc                                                                       >  1:1174197/1‑140 (MQ=255)

                                                           ccTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCAccc                                                                       >  2:792818/1‑140 (MQ=255)

                                                           ccTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCAccc                                                                       >  2:457223/1‑140 (MQ=255)

                                                             tACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGca                                                                             <  1:472377/132‑1 (MQ=255)

                                                             tACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGca                                                                             >  2:472377/1‑132 (MQ=255)

                                                             tACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTg                                                                     >  2:318669/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACTGGCTGGATCCATCGCACCACCCTGct                                                                   >  1:30773/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGct                                                                   >  1:638567/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGct                                                                   >  2:251201/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGct                                                                   >  1:325181/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGct                                                                   >  2:25692/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGct                                                                   >  1:340604/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGct                                                                   >  2:567792/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGct                                                                   >  2:682340/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGct                                                                   >  1:782369/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGct                                                                   >  1:423320/1‑140 (MQ=255)

                                                               caacaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGct                                                                   >  2:768446/1‑140 (MQ=255)

                                                                 acaGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGctct                                                                 >  1:1127775/1‑140 (MQ=255)

                                                                   aGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTcg                                                               <  2:737828/140‑1 (MQ=255)

                                                                     gCTGGTGGCTTAAGCCCTCAGGAAAAGCAGATCCCCCGTAGCCCACGTGACATTGTGATGCTGCAGCACAAAGCCAGCAACACAGGACAGGGCGTAGGCAATACCACCGGCTCGTTCCATAGCACCACCCTGCTCTcgcg                                                             >  2:468171/1‑140 (MQ=255)

                                                                     gCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGca                                                                             <  2:280082/124‑1 (MQ=255)

                                                                     gCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGca                                                                             >  1:280082/1‑124 (MQ=255)

                                                                     gCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTcgcg                                                             >  1:846782/1‑140 (MQ=255)

                                                                     gCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTcgcg                                                             <  1:77464/140‑1 (MQ=255)

                                                                     gCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTcgcg                                                             <  1:940709/140‑1 (MQ=255)

                                                                     gCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTcgcg                                                             <  1:567792/140‑1 (MQ=255)

                                                                     gCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTC‑CGCgg                                                            >  1:228507/1‑140 (MQ=255)

                                                                       tggtggCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCAccc                                                                       <  1:360563/128‑1 (MQ=255)

                                                                       tggtggCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCAccc                                                                       >  2:360563/1‑128 (MQ=255)

                                                                          tggCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGgtg                                                        >  1:319580/1‑140 (MQ=255)

                                                                           ggCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGgtgt                                                       <  1:321364/140‑1 (MQ=255)

                                                                           ggCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGgtgt                                                       <  1:1347861/140‑1 (MQ=255)

                                                                                     cGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATc                                             <  1:984092/140‑1 (MQ=255)

                                                                                     cGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATc                                             <  2:638567/140‑1 (MQ=255)

                                                                                      gCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATcc                                            <  1:76625/140‑1 (MQ=255)

                                                                                      gCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATcc                                            <  2:447653/140‑1 (MQ=255)

                                                                                      gCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATcc                                            >  2:289078/1‑140 (MQ=255)

                                                                                       cAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCggg                                                           <  1:396491/124‑1 (MQ=255)

                                                                                       cAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCggg                                                           >  2:396491/1‑124 (MQ=255)

                                                                                       cAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATccc                                           >  1:1099038/1‑140 (MQ=255)

                                                                                            aaTGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCg                                      <  1:1193946/140‑1 (MQ=255)

                                                                                                  gATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGtt                                <  2:340604/140‑1 (MQ=255)

                                                                                                  gATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGtt                                >  1:529408/1‑140 (MQ=255)

                                                                                                  gATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGtt                                >  2:806603/1‑140 (MQ=255)

                                                                                                    tCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTAt                              <  1:185654/140‑1 (MQ=255)

                                                                                                    tCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTAt                              >  1:723386/1‑140 (MQ=255)

                                                                                                      ccccGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCa                            >  1:403576/1‑140 (MQ=255)

                                                                                                      ccccGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCa                            >  1:613703/1‑140 (MQ=255)

                                                                                                      ccccGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCa                            >  2:587915/1‑140 (MQ=255)

                                                                                                        ccGTAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCaga                          >  1:1137234/1‑140 (MQ=255)

                                                                                                           tAGCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCagaaga                       >  2:198130/1‑140 (MQ=255)

                                                                                                             gCCCACGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGAtt                     >  1:362502/1‑140 (MQ=255)

                                                                                                                 aCGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACg                 <  1:250344/140‑1 (MQ=255)

                                                                                                                 aCGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACg                 >  1:290985/1‑140 (MQ=255)

                                                                                                                  cGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGa                >  1:904721/1‑140 (MQ=255)

                                                                                                                  cGGCAGATTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGa                >  1:113870/1‑140 (MQ=255)

                                                                                                                        aTTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACgg          <  2:529408/140‑1 (MQ=255)

                                                                                                                        aTTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACgg          <  2:1099038/140‑1 (MQ=255)

                                                                                                                        aTTGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACgg          <  2:219720/140‑1 (MQ=255)

                                                                                                                         ttGTGATGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACggg         <  2:325181/140‑1 (MQ=255)

                                                                                                                              aTGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACgg          <  1:299897/134‑1 (MQ=255)

                                                                                                                              aTGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACgg          >  2:299897/1‑134 (MQ=255)

                                                                                                                              aTGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCa    <  1:682340/140‑1 (MQ=255)

                                                                                                                               tGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCAt   <  1:792818/140‑1 (MQ=255)

                                                                                                                               tGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCAt   <  1:282030/140‑1 (MQ=255)

                                                                                                                               tGCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCAt   >  1:1094512/1‑140 (MQ=255)

                                                                                                                                gCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACTGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCAtg  <  2:30773/140‑1 (MQ=255)

                                                                                                                                gCTCCAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCAtg  <  1:806603/140‑1 (MQ=255)

                                                                                                                                   ccAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGc     >  2:765932/1‑134 (MQ=255)

                                                                                                                                   ccAGCACAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGc     <  1:765932/134‑1 (MQ=255)

GGCGAATCCACCAGCCAGAATATCGCCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACAGGCTGGTGGCTTAAGCCCGCAGGGAATGCAGATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCAGCAAACCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATGATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATG  >  NC_000913/3233216‑3233483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A