Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 159 82.1 2830106 99.3% 2810295 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,246,806 C→T A52V (GCC→GTC)  exuR → DNA‑binding transcriptional repressor ExuR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,246,8060CT96.0% 247.6 / 0.5 76A52V (GCC→GTC) exuRDNA‑binding transcriptional repressor ExuR
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (30/43);  total (32/44)
Fisher's exact test for biased strand distribution p-value = 5.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

CGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACGGTCCGTTTGAGT  >  NC_000913/3246670‑3246940
                                                                                                                                        |                                                                                                                                      
cGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTcat                                                                                                                                     >  1:1008585/1‑140 (MQ=255)
cGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTcat                                                                                                                                     >  1:105931/1‑140 (MQ=255)
  ccGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGtcatca                                                                                                                                   <  2:664661/140‑1 (MQ=255)
    gTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTcatcatc                                                                                                                                 <  2:481713/140‑1 (MQ=255)
        gTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGc                                                                                                                             <  1:1081373/140‑1 (MQ=255)
           tCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTgg                                                                                                                          <  2:1008585/140‑1 (MQ=255)
           tCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTgg                                                                                                                          <  2:680546/140‑1 (MQ=255)
           tCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTgg                                                                                                                          >  1:1176428/1‑140 (MQ=255)
           tCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTgg                                                                                                                          <  2:1176428/140‑1 (MQ=255)
                 acTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTg                                                                                                                    <  1:602647/140‑1 (MQ=255)
                   ttGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGaa                                                                                                                  >  2:1398149/1‑140 (MQ=255)
                     gCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAgg                                                                                                                >  2:116851/1‑140 (MQ=255)
                     gCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAgg                                                                                                                >  1:274077/1‑140 (MQ=255)
                     gCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAgg                                                                                                                <  1:971571/140‑1 (MQ=255)
                       cGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCt                                                                                                              <  1:1271409/140‑1 (MQ=255)
                       cGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCt                                                                                                              <  2:1167065/140‑1 (MQ=255)
                            aCCAGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTc                                                                                                         <  1:1366033/140‑1 (MQ=255)
                             ccTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCg                                                                                                        <  2:520980/140‑1 (MQ=255)
                             ccTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCg                                                                                                        <  2:105931/140‑1 (MQ=255)
                             ccTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCg                                                                                                        <  2:518970/140‑1 (MQ=255)
                             ccTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCg                                                                                                        <  1:1067586/140‑1 (MQ=255)
                               tGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGaa                                                                                                      <  2:738338/140‑1 (MQ=255)
                               tGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGaa                                                                                                      <  1:690247/140‑1 (MQ=255)
                                  aaGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTg                                                                                                   <  2:929507/140‑1 (MQ=255)
                                       cgcATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTaa                                                                                              <  1:572135/140‑1 (MQ=255)
                                       cgcATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTaa                                                                                              >  1:769293/1‑140 (MQ=255)
                                           tCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCATCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGt                                                                                          <  2:1284189/140‑1 (MQ=255)
                                           tCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGt                                                                                          <  1:1117049/140‑1 (MQ=255)
                                             gAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCCGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTc                                                                                        <  1:551882/140‑1 (MQ=255)
                                               aCAGGGCGTCTATCTGGTGGGTGTTAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAg                                                                                      >  1:938354/1‑140 (MQ=255)
                                                   ggCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTAt                                                                                  >  1:164948/1‑140 (MQ=255)
                                                        cTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCAtg                                                                             <  2:51548/140‑1 (MQ=255)
                                                           tCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTgg                                                                          >  2:13922/1‑140 (MQ=255)
                                                             tggtggGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGtt                                                                        >  1:1094758/1‑140 (MQ=255)
                                                             tggtggGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGtt                                                                        >  1:1141619/1‑140 (MQ=255)
                                                               gtgggtgATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCAtgt                                                                            <  2:308130/134‑1 (MQ=255)
                                                               gtgggtgATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCAtgt                                                                            >  1:308130/1‑134 (MQ=255)
                                                                tgggtgATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTcc                                                                     >  1:470604/1‑140 (MQ=255)
                                                                  ggtgATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTATGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCaa                                                                   >  2:1189190/1‑140 (MQ=255)
                                                                  ggtgATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCaa                                                                   >  1:818154/1‑140 (MQ=255)
                                                                             gcctgcAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGCTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGcc                                                        >  1:241603/1‑140 (MQ=255)
                                                                              cctgcAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCa                                                       >  1:822178/1‑140 (MQ=255)
                                                                              cctgcAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCa                                                       >  1:282504/1‑140 (MQ=255)
                                                                              cctgcAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCa                                                       >  1:983516/1‑140 (MQ=255)
                                                                               ctgcAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCAt                                                      <  2:938354/140‑1 (MQ=255)
                                                                                tgcAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATc                                                     >  1:396937/1‑140 (MQ=255)
                                                                                      aCGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAgg                                               <  1:116851/140‑1 (MQ=255)
                                                                                      aCGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAgg                                               <  2:482610/140‑1 (MQ=255)
                                                                                         cTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAggcgg                                            <  2:164948/140‑1 (MQ=255)
                                                                                         cTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAggcgg                                            <  2:748397/140‑1 (MQ=255)
                                                                                           ttATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCt                                          <  1:1142646/140‑1 (MQ=255)
                                                                                            tATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCcgcg                                                          <  1:768156/123‑1 (MQ=255)
                                                                                            tATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCcgcg                                                          >  2:768156/1‑123 (MQ=255)
                                                                                            tATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTg                                         >  1:839090/1‑140 (MQ=255)
                                                                                             aTTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGa                                        <  2:769293/140‑1 (MQ=255)
                                                                                               tGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACa                                      <  2:1094758/140‑1 (MQ=255)
                                                                                               tGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACa                                      >  2:731416/1‑140 (MQ=255)
                                                                                                gCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACaa                                     <  2:470604/140‑1 (MQ=255)
                                                                                                 ccGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACa                                      >  2:545240/1‑138 (MQ=255)
                                                                                                 ccGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACa                                      <  1:545240/138‑1 (MQ=255)
                                                                                                   gATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACaataa                                  >  1:352140/1‑140 (MQ=255)
                                                                                                    aTGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACa                                      >  1:1051553/1‑135 (MQ=255)
                                                                                                    aTGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACa                                      <  2:1051553/135‑1 (MQ=255)
                                                                                                     tGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAataata                                <  1:357235/140‑1 (MQ=255)
                                                                                                            aaCGTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAAtt                         <  1:13922/140‑1 (MQ=255)
                                                                                                               gTCAGCCGTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGc                      <  1:628960/140‑1 (MQ=255)
                                                                                                                  aGCCGTACGGTAGTTCGTGAAGTCATAATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCaa                   >  1:735585/1‑140 (MQ=255)
                                                                                                                      gTACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTAc               >  2:179271/1‑140 (MQ=255)
                                                                                                                       tACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCTGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACg              <  2:274077/140‑1 (MQ=255)
                                                                                                                       tACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACg              <  2:1129368/140‑1 (MQ=255)
                                                                                                                       tACGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACg              <  1:179271/140‑1 (MQ=255)
                                                                                                                        aCGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGAATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACgg             <  2:983516/140‑1 (MQ=255)
                                                                                                                         cGGTAGTTCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACGGt            <  2:822178/140‑1 (MQ=255)
                                                                                                                               ttCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACGGTCCGttt      >  1:528474/1‑140 (MQ=255)
                                                                                                                               ttCGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACGGTCCGttt      >  1:271149/1‑140 (MQ=255)
                                                                                                                                 cGTGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACGGTCCGTTTGa    >  2:169732/1‑140 (MQ=255)
                                                                                                                                   tGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACGGTCCGTTTGAGt  <  2:528474/140‑1 (MQ=255)
                                                                                                                                   tGAAGTCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACGGTCCGTTTGAGt  <  2:696803/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGAGCGCATCGAACAGGGCGTCTATCTGGTGGGTGATAAACTGCCTGCAGAACGCTTTATTGCCGATGAAAAGAACGTCAGCCGTACGGTAGTTCGTGAAGCCATCATCATGCTGGAAGTTGAAGGCTATGTCGAAGTGCGTAAAGGTTCAGGTATTCATGTGGTTTCCAACCAGCCGCGCCATCAGCAGGCGGCTGACAATAATATGGAATTCGCCAATTACGGTCCGTTTGAGT  >  NC_000913/3246670‑3246940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A