Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 159 82.1 2830106 99.3% 2810295 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,261,403 G→A A339V (GCG→GTG)  tdcE ← 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,261,4030GA100.0% 356.6 / NA 104A339V (GCG→GTG) tdcE2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/55);  total (49/55)

Only 100 of 106 total aligned reads displayed.

CCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCG  >  NC_000913/3261275‑3261539
                                                                                                                                |                                                                                                                                        
ccGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTggg                                                                                                                               >  2:710951/1‑140 (MQ=255)
               tCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGc                                                                                                                <  1:1266250/140‑1 (MQ=255)
                           ccGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTcc                                                                                                    >  2:39755/1‑140 (MQ=255)
                             ggTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCgg                                                                                                  >  1:604649/1‑140 (MQ=255)
                             ggTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCgg                                                                                                  >  2:1089380/1‑140 (MQ=255)
                             ggTGCCGGCCCCATAGTGTGCAAGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCgg                                                                                                  >  2:525579/1‑140 (MQ=255)
                               tGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGCCCGTCCAGCCCCATCCCGCCGATCACTTCCGCCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGgtg                                                                                                <  1:616083/140‑1 (MQ=255)
                               tGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCACCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGgtg                                                                                                <  1:332730/140‑1 (MQ=255)
                                     ccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCa                                                                                          <  1:1229898/140‑1 (MQ=255)
                                         aTAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTGCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGaa                                                                                      <  2:1237296/140‑1 (MQ=255)
                                            ttgtgCAGGTTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   <  1:1269119/139‑1 (MQ=255)
                                            gtgtGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   <  1:886433/140‑1 (MQ=255)
                                              gtgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTa                                                                                 <  1:1335188/140‑1 (MQ=255)
                                              gtgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTa                                                                                 <  2:107075/140‑1 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCTCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTAc                                                                                >  2:843032/1‑140 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGaa                                                                                      >  2:752374/1‑134 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGaa                                                                                      <  1:752374/134‑1 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   <  2:1278229/137‑1 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   <  1:836284/137‑1 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   >  2:287640/1‑137 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   <  1:287640/137‑1 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   >  2:836284/1‑137 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   >  1:1278229/1‑137 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTAc                                                                                >  1:1205963/1‑140 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTAc                                                                                >  2:429647/1‑140 (MQ=255)
                                               tgCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTAc                                                                                >  1:1295003/1‑140 (MQ=255)
                                               tgCAGGGGGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCACCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACCGCGAATCAAATTCCGGTGTACGCAGGAAGCGTAc                                                                                >  2:75639/1‑140 (MQ=255)
                                                  aGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCGGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCAt                                                                             >  1:748715/1‑140 (MQ=255)
                                                  aGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCAt                                                                             <  1:1332849/140‑1 (MQ=255)
                                                  aGGGTGTGCAAATAGCGGAAGGAGTTTTTGGGCACCAGCGTCCGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCAt                                                                             <  1:1276237/140‑1 (MQ=255)
                                                   gggTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATa                                                                            >  2:818273/1‑140 (MQ=255)
                                                    ggTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGc                                                                                           <  2:527314/124‑1 (MQ=255)
                                                    ggTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGc                                                                                           >  1:527314/1‑124 (MQ=255)
                                                    ggTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   >  1:262739/1‑132 (MQ=255)
                                                    ggTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   <  2:262739/132‑1 (MQ=255)
                                                    ggTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATAc                                                                           >  2:274264/1‑140 (MQ=255)
                                                     gtgtGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACg                                                                          <  1:525579/140‑1 (MQ=255)
                                                     gtgtGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACg                                                                          <  1:39755/140‑1 (MQ=255)
                                                      tgtgTAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACgg                                                                         >  2:267560/1‑140 (MQ=255)
                                                      tgtgCAAATAGCTGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACTGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACgg                                                                         >  1:871094/1‑140 (MQ=255)
                                                      tgtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   >  2:812244/1‑130 (MQ=255)
                                                      tgtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   <  1:812244/130‑1 (MQ=255)
                                                      tgtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACgg                                                                         >  2:593392/1‑140 (MQ=255)
                                                       gtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   <  1:960106/129‑1 (MQ=255)
                                                       gtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCg                                                                                   >  2:960106/1‑129 (MQ=255)
                                                       gtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGa                                                                        >  1:248151/1‑140 (MQ=255)
                                                       gtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGa                                                                        >  1:162892/1‑140 (MQ=255)
                                                       gtgCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGa                                                                        >  1:1337673/1‑140 (MQ=255)
                                                         gCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGaa                                                                                      <  2:1149563/124‑1 (MQ=255)
                                                         gCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGaa                                                                                      >  1:1149563/1‑124 (MQ=255)
                                                         gCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATc                                                                      >  1:1358534/1‑140 (MQ=255)
                                                          cAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCt                                                                     <  1:593392/140‑1 (MQ=255)
                                                          cAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCt                                                                     <  1:818273/140‑1 (MQ=255)
                                                            aaTAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTc                                                                   <  1:579084/140‑1 (MQ=255)
                                                              tAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCat                                                                 >  2:1350127/1‑140 (MQ=255)
                                                              tAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCat                                                                 >  1:48256/1‑140 (MQ=255)
                                                              tAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCat                                                                 >  2:1013333/1‑140 (MQ=255)
                                                                gCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCt                                                                     <  2:1242525/134‑1 (MQ=255)
                                                                gCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCt                                                                     >  1:1242525/1‑134 (MQ=255)
                                                                gCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCatga                                                               >  1:351311/1‑140 (MQ=255)
                                                                  ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCatgatg                                                             <  1:1283243/140‑1 (MQ=255)
                                                                  ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCatgatg                                                             >  2:324933/1‑140 (MQ=255)
                                                                  ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCatgatg                                                             <  2:1205963/140‑1 (MQ=255)
                                                                  ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCatgatg                                                             <  2:689714/140‑1 (MQ=255)
                                                                   gAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCGGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAtgatga                                                            <  2:748715/140‑1 (MQ=255)
                                                                   gAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAtgatga                                                            <  2:48256/140‑1 (MQ=255)
                                                                     aGGAGTTTTTGGTCACCAGCGTACGACCGCCCAGCCCCACCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAg                                                          <  1:924044/140‑1 (MQ=255)
                                                                         gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgat                                                      <  1:1211785/140‑1 (MQ=255)
                                                                         gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgat                                                      <  2:769098/140‑1 (MQ=255)
                                                                             ttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcgat                                                  <  2:1358534/140‑1 (MQ=255)
                                                                             ttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcgat                                                  <  2:1295003/140‑1 (MQ=255)
                                                                              tGGTCACCAGCGTACGCCCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTgatcgatc                                                 <  2:1231032/140‑1 (MQ=255)
                                                                                  cACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGtt                                             >  2:121355/1‑140 (MQ=255)
                                                                                     caccGTACGACCGTCCACCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAATAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCt                                          <  2:1227936/137‑1 (MQ=255)
                                                                                             gACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgc                                  <  1:142644/140‑1 (MQ=255)
                                                                                             gACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgc                                  <  2:533792/140‑1 (MQ=255)
                                                                                             gACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgc                                  <  1:1350127/140‑1 (MQ=255)
                                                                                             gACCGTCCAGCCCCATCCCGACGATCACTTCCGTCACCCAGATTGTGTCGCCGGAGATCAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATTCGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgc                                  <  2:1247080/140‑1 (MQ=255)
                                                                                              aCCGTCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCctgct                                 <  2:885029/140‑1 (MQ=255)
                                                                                                  tCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTc                             >  2:1366525/1‑140 (MQ=255)
                                                                                                  tCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTc                             >  2:424713/1‑140 (MQ=255)
                                                                                                  tCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTc                             >  1:503498/1‑140 (MQ=255)
                                                                                                  tCCAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGAGCAGTTCCTGTGCCTGCTGCTc                             >  2:1287861/1‑140 (MQ=255)
                                                                                                   ccAGCCCCATCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCa                            <  2:248151/140‑1 (MQ=255)
                                                                                                            tCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACg                   >  2:84572/1‑140 (MQ=255)
                                                                                                            tCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACg                   >  1:630269/1‑140 (MQ=255)
                                                                                                            tCCCGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACg                   >  2:711571/1‑140 (MQ=255)
                                                                                                             cccGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGc                  <  2:378202/140‑1 (MQ=255)
                                                                                                             cccGCCGATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGc                  <  1:267560/140‑1 (MQ=255)
                                                                                                              ccgccgATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGcc                 <  1:1013333/140‑1 (MQ=255)
                                                                                                               cgccgATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCa                >  1:679631/1‑140 (MQ=255)
                                                                                                                 ccgATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCCTCATGATGAAGTGATCGATCAGTTCCTGTGCctgctg                                >  1:347528/1‑122 (MQ=255)
                                                                                                                 ccgATCACTTCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCCTCATGATGAAGTGATCGATCAGTTCCTGTGCctgctg                                <  2:347528/122‑1 (MQ=255)
                                                                                                                        cTTCCGTCACCCAGATTGGATCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGcc                 <  1:269828/130‑1 (MQ=255)
                                                                                                                        cTTCCGTCACCCAGATTGGATCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGcc                 >  2:269828/1‑130 (MQ=255)
                                                                                                                         ttCCGTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGt      >  1:443679/1‑140 (MQ=255)
                                                                                                                             gTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTcgcg  <  2:604649/140‑1 (MQ=255)
                                                                                                                             gTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTcgcg  <  1:324933/140‑1 (MQ=255)
                                                                                                                             gTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTcgcg  <  2:503498/140‑1 (MQ=255)
                                                                                                                             gTCACCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTcgcg  <  1:84572/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                        
CCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGATGAAGTGATCGATCAGTTCCTGTGCCTGCTGCTCATTGAGTACGCCAGCTTTAAAGTCGCG  >  NC_000913/3261275‑3261539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A