Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 159 82.1 2830106 99.3% 2810295 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,114,992 A→G C196C (TGT→TGC)  glpX ← fructose‑1,6‑bisphosphatase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,114,9920AG98.5% 236.9 / ‑4.4 65C196C (TGT→TGC) glpXfructose‑1,6‑bisphosphatase 2
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (50/14);  total (51/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01

TGTCGCCTTTGACGTCATGACGCGCCAGCAGACGACCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATACAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCAGTTCGCTCAACGGTTTGC  >  NC_000913/4114856‑4115128
                                                                                                                                        |                                                                                                                                        
tGTCGCCTTTGACGTCATGACGCGCCAGCAGACGACCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAg                                                                                                                                       >  2:1378610/1‑140 (MQ=255)
 gTCGCTTTTGATGTCATGCCGCGCCAGCAGACGCCCGTTCATGTCCCCATCAAATGCGGGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAgg                                                                                                                                      <  2:1040517/140‑1 (MQ=255)
       tttGACGTCATGACGCGCCAACAGACGACCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGaa                                                                                                                                >  2:583044/1‑140 (MQ=255)
              tCATGACGCGCCAGCAGACGACCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGc                                                                                                                         >  2:495137/1‑140 (MQ=255)
                aTGACGCGCCAGCAGACGACCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCg                                                                                                                       >  2:993507/1‑140 (MQ=255)
                  gACGCGCCAGCAGACGACCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCa                                                                                                                     >  1:323399/1‑140 (MQ=255)
                       gcCAGCAGACGACCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTc                                                                                                                >  1:272223/1‑140 (MQ=255)
                        ccAGCAGACGCCCTTTCATGCCGCCATCTAAGGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCg                                                                                                               <  2:1365458/140‑1 (MQ=255)
                        ccAGCAGACGACCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCg                                                                                                               <  2:830589/140‑1 (MQ=255)
                        caagcagACGCCCGTTCATGTCGCCATCTAAGGCGGGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCg                                                                                                               <  2:272223/138‑1 (MQ=255)
                         cagcagACGACCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGc                                                                                                              >  1:418226/1‑140 (MQ=255)
                           gcagACGCCCTTTCATGCCGCCATCAAATGCGCGGACCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCg                                                                                                            <  2:163738/140‑1 (MQ=255)
                                  acCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGaa                                                                                                     <  2:654935/140‑1 (MQ=255)
                                   ccGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAAt                                                                                                    >  1:440626/1‑140 (MQ=255)
                                     gTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAg                                                                                                  >  2:309470/1‑140 (MQ=255)
                                                cATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGt                                                                                       >  1:279738/1‑140 (MQ=255)
                                                 aTCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTa                                                                                      >  2:339452/1‑140 (MQ=255)
                                                         cgcgGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGt                                                                              >  2:712409/1‑140 (MQ=255)
                                                         cgcgGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGt                                                                              >  2:1281411/1‑140 (MQ=255)
                                                          gcgGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGtt                                                                             >  1:445295/1‑140 (MQ=255)
                                                            ggATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTtgc                                                                           >  1:568798/1‑140 (MQ=255)
                                                            ggATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTtgc                                                                           >  2:1290826/1‑140 (MQ=255)
                                                             gctccccgccgCAGAAACTACGCCTCCCGGCGCGCCACCAATTCCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTtgct                                                                          <  2:1160804/135‑1 (MQ=255)
                                                              aTCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTtgctg                                                                         <  2:1145244/140‑1 (MQ=255)
                                                                     ccgcAGAAACTACGCCTTCCGGCGCGCCGCCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCa                                                                  <  2:1310562/140‑1 (MQ=255)
                                                                       gcAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGc                                                                <  1:993507/140‑1 (MQ=255)
                                                                        cAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGGATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTtgctg                                                                         >  2:872154/1‑130 (MQ=255)
                                                                        cAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTtgctg                                                                         <  1:872154/130‑1 (MQ=255)
                                                                        cAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCg                                                               >  1:712716/1‑140 (MQ=255)
                                                                          gAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGAt                                                             >  1:1021111/1‑140 (MQ=255)
                                                                                  gCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCAt                                                     >  2:1086880/1‑140 (MQ=255)
                                                                                       ccGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATcgtgg                                                >  1:929566/1‑140 (MQ=255)
                                                                                        cGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATcgtggc                                               <  2:445295/140‑1 (MQ=255)
                                                                                        cGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATcgtggc                                               <  1:309470/140‑1 (MQ=255)
                                                                                             cgcCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGt                                          >  2:1212849/1‑140 (MQ=255)
                                                                                              gcCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGtt                                         >  1:1169382/1‑140 (MQ=255)
                                                                                                caccaATACCGTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTa                                       >  2:72851/1‑140 (MQ=255)
                                                                                                          gTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCg                             >  1:1163280/1‑140 (MQ=255)
                                                                                                          gTACAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATCGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCg                             >  2:456947/1‑140 (MQ=255)
                                                                                                            aCAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGtt                           >  1:988774/1‑140 (MQ=255)
                                                                                                            aCAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGtt                           >  1:215022/1‑140 (MQ=255)
                                                                                                             cAGCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTa                          >  2:860772/1‑140 (MQ=255)
                                                                                                               gCACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTAcc                        >  1:1131483/1‑140 (MQ=255)
                                                                                                                cACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCg                       >  1:344236/1‑140 (MQ=255)
                                                                                                                cACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCg                       >  2:263648/1‑140 (MQ=255)
                                                                                                                cACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCg                       >  1:405975/1‑140 (MQ=255)
                                                                                                                cACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCg                       >  1:766629/1‑140 (MQ=255)
                                                                                                                cACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCg                       >  2:629467/1‑140 (MQ=255)
                                                                                                                cACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCg                       >  1:137149/1‑140 (MQ=255)
                                                                                                                cACGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCg                       >  1:968255/1‑140 (MQ=255)
                                                                                                                  cGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTc                     >  2:253474/1‑140 (MQ=255)
                                                                                                                  cGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTc                     >  1:310603/1‑140 (MQ=255)
                                                                                                                  cGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTc                     >  2:16284/1‑140 (MQ=255)
                                                                                                                  cGTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTc                     >  2:269849/1‑140 (MQ=255)
                                                                                                                   gTCAACTTCGCTGTCTGGCATGCGGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCa                    >  2:1256862/1‑140 (MQ=255)
                                                                                                                   gTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCa                    >  1:389476/1‑140 (MQ=255)
                                                                                                                   gTCAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCa                    >  2:780903/1‑140 (MQ=255)
                                                                                                                     cAACTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCAGt                  >  2:1196298/1‑140 (MQ=255)
                                                                                                                        cTTCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCAGTTCg               >  2:528434/1‑140 (MQ=255)
                                                                                                                          tCGCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCAGTTCGCt             >  2:1229852/1‑140 (MQ=255)
                                                                                                                          tCGCTGTCTGGCATACAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCAGTTCGCt             >  2:1240094/1‑140 (MQ=255)
                                                                                                                            gCTGTCTGGCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCAGTTCGCTCa           <  1:1290826/140‑1 (MQ=255)
                                                                                                                                   ggCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCAGTTCGCTCAACGGttt    >  2:570718/1‑140 (MQ=255)
                                                                                                                                   ggCATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCAGTTCGCTCAACGGttt    >  2:1245469/1‑140 (MQ=255)
                                                                                                                                     cATGCAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCAGTTCGCTCAACGGTTTGc  >  2:1354581/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TGTCGCCTTTGACGTCATGACGCGCCAGCAGACGACCGTTCATGTCGCCATCTAATGCGCGGATCACCGCCGCAGAAACTACGCCTTCCGGCGCGCCACCAATACCGTACAGCACGTCAACTTCGCTGTCTGGCATACAGGTGAGAATTGAGGCCGCAACGTCGCCGTCCGGAATAGCAAATACGCGTACGCCGAGTTGCTGCATTTCAGCGATAACGGCATCGTGGCGTGGTTTAGCCAGAATCGTTACCGTCAGTTCGCTCAACGGTTTGC  >  NC_000913/4114856‑4115128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A