Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,855	G→A	A79V (GCC→GTC)	glpF ←	glycerol facilitator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,855	0	G	A	100.0%	244.0 / NA	69	A79V (GCC→GTC)	glpF	glycerol facilitator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/35); total (34/35)

GCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAG  >  NC_000913/4117719‑4117978

gCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACaa                                                                                                                          >  2:737279/1‑140 (MQ=255)

    cgAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAg                                                                                                                      <  1:492677/140‑1 (MQ=255)

     gAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGc                                                                                                                     <  2:757756/140‑1 (MQ=255)

     gAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGc                                                                                                                     <  1:1320742/140‑1 (MQ=255)

        cAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCcac                                                                                                                  <  2:992747/140‑1 (MQ=255)

        cAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCcac                                                                                                                  <  1:252511/140‑1 (MQ=255)

         aaTGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCcaca                                                                                                                 <  2:654416/140‑1 (MQ=255)

           tgtgATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCAcaat                                                                                                               >  2:1126046/1‑140 (MQ=255)

           tgtgATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCAcaat                                                                                                               <  2:1241309/140‑1 (MQ=255)

           tgtgATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCAcaat                                                                                                               <  2:598641/140‑1 (MQ=255)

           tgtgATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCAcaat                                                                                                               >  1:1241309/1‑140 (MQ=255)

              gatgaGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCAcaatgca                                                                                                            <  1:375235/140‑1 (MQ=255)

                tgaGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAAc                                                                                                                        <  1:1363771/126‑1 (MQ=255)

                tgaGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAAc                                                                                                                        <  1:410003/126‑1 (MQ=255)

                tgaGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAAc                                                                                                                        >  2:1363771/1‑126 (MQ=255)

                tgaGTCTGCTCGAAGTCGAAAAATAAACTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAAc                                                                                                                        >  2:410003/1‑126 (MQ=255)

                    tCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTa                                                                                                      >  2:318160/1‑140 (MQ=255)

                     cTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTaa                                                                                                     >  1:1375335/1‑140 (MQ=255)

                     cTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTaa                                                                                                     <  2:1105859/140‑1 (MQ=255)

                      tGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAAc                                                                                                    >  2:975948/1‑140 (MQ=255)

                       gCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGt                                                                                                       >  2:410867/1‑136 (MQ=255)

                       gCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGt                                                                                                       <  1:410867/136‑1 (MQ=255)

                       gCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACa                                                                                                   >  1:932711/1‑140 (MQ=255)

                        cTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCTGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAg                                                                                                  <  1:908392/140‑1 (MQ=255)

                        cTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAg                                                                                                  <  2:525991/140‑1 (MQ=255)

                        cTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAg                                                                                                  >  2:146862/1‑140 (MQ=255)

                        cTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAg                                                                                                  <  2:668464/140‑1 (MQ=255)

                        cTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAg                                                                                                  >  2:367891/1‑140 (MQ=255)

                        cTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAg                                                                                                  <  1:383754/140‑1 (MQ=255)

                             aGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGa                                                                                             >  2:320394/1‑140 (MQ=255)

                                  aaaaaTAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAg                                                                                        >  2:445411/1‑140 (MQ=255)

                                     aataaatTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATg                                                                                     <  1:450702/140‑1 (MQ=255)

                                                 taAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAAc                                                                         >  2:179162/1‑140 (MQ=255)

                                                 taAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAAc                                                                         >  2:498061/1‑140 (MQ=255)

                                                      cccGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTg                                                                    >  2:524702/1‑140 (MQ=255)

                                                      cccGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTg                                                                    >  2:539290/1‑140 (MQ=255)

                                                      cccGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTg                                                                    >  2:571174/1‑140 (MQ=255)

                                                      cccGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTg                                                                    <  1:1126046/140‑1 (MQ=255)

                                                      cccGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTg                                                                    <  1:571174/140‑1 (MQ=255)

                                                      cccGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTg                                                                    >  1:133607/1‑140 (MQ=255)

                                                        cGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCTCGCCGGAAACCCCTGCg                                                                  >  2:1258707/1‑140 (MQ=255)

                                                        cGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGc                                                                   <  2:780246/139‑1 (MQ=255)

                                                        cGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGc                                                                   >  1:780246/1‑139 (MQ=255)

                                                        cGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCg                                                                  >  2:71468/1‑140 (MQ=255)

                                                        cGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCg                                                                  <  1:318160/140‑1 (MQ=255)

                                                        cGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCg                                                                  <  1:872504/140‑1 (MQ=255)

                                                        cGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCg                                                                  >  2:1263782/1‑140 (MQ=255)

                                                         gTAAACTAAAGCCGCAGCACAGAAAGCGCCCGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCgg                                                                 >  2:105708/1‑140 (MQ=255)

                                                          tAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGaaa                                                                          >  2:157831/1‑130 (MQ=255)

                                                          tAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGaaa                                                                          <  1:157831/130‑1 (MQ=255)

                                                           aaaCTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTc                                                               >  1:6396/1‑140 (MQ=255)

                                                                  aGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGa                                                        >  1:621266/1‑140 (MQ=255)

                                                                    ccGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATg                                                      >  1:52627/1‑140 (MQ=255)

                                                                        agcaCAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCa                                                  <  1:539290/140‑1 (MQ=255)

                                                                        agcaCAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCa                                                  <  2:133607/140‑1 (MQ=255)

                                                                             aGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGcc                                             <  1:320394/140‑1 (MQ=255)

                                                                                  gcgcCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCAcccc                                        <  1:1244221/140‑1 (MQ=255)

                                                                                  gcgcCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCAcccc                                        <  1:498061/140‑1 (MQ=255)

                                                                                  gcgcCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCAcccc                                        <  1:179162/140‑1 (MQ=255)

                                                                                      cGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGt                                    <  1:975948/140‑1 (MQ=255)

                                                                                       ggCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTc                                   >  1:254635/1‑140 (MQ=255)

                                                                                        gCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTcc                                  <  1:737279/140‑1 (MQ=255)

                                                                                             ttGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCaaa                             >  1:609615/1‑140 (MQ=255)

                                                                                                   aaCGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGacac                       <  1:1263782/140‑1 (MQ=255)

                                                                                                    aCGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACt                      <  1:1073511/140‑1 (MQ=255)

                                                                                                     cGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTg                     >  1:928720/1‑140 (MQ=255)

                                                                                                                 tAACTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGa         >  2:446777/1‑140 (MQ=255)

                                                                                                                  aaCTTTGCGCTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGAc        <  2:928720/140‑1 (MQ=255)

                                                                                                                        gcgcTTGTCGAAACAGACAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAg  >  2:160895/1‑140 (MQ=255)

GCCGCGAACAATGTGATGAGTCTGCTCGAAGTCGAAAAATAAATTGTAGTAAAGCCCGTAAACTAAAGCCGCAGCACAGAAAGCGCCGGCAACTTGTGAAACGATAAAAGGAATAACTTTGCGCTTGTCGAAACAGGCAAACAGCCACAATGCAATGGTAACAGCGGGATTAAGATGCGCGCCGGAAACCCCTGCGGTCAGGTAGATGGCCATTGCCACCCCCAGTCCCCAAATGACACTGATTTCCCACTGACCAAAAG  >  NC_000913/4117719‑4117978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A