Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 159 82.1 2830106 99.3% 2810295 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,271,009 C→T L118F (CTC→TTC)  yjbR → PF04237 family protein YjbR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,271,0090CT100.0% 170.0 / NA 49L118F (CTC→TTC) yjbRPF04237 family protein YjbR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/21);  total (28/21)

TCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTG  >  NC_000913/4270873‑4271145
                                                                                                                                        |                                                                                                                                        
tCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAAttct                                                                                                                                       <  1:1342933/140‑1 (MQ=255)
  tGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGa                                                                                                                                     <  1:1018486/140‑1 (MQ=255)
             cACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGc                                                                                                                          <  2:942388/140‑1 (MQ=255)
              aCTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGcc                                                                                                                         >  1:379594/1‑140 (MQ=255)
              aCTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGcc                                                                                                                         <  1:1397850/140‑1 (MQ=255)
                   aGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCa                                                                                                                    >  1:561002/1‑140 (MQ=255)
                     cACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGa                                                                                                                  >  2:263665/1‑140 (MQ=255)
                     cACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGa                                                                                                                  >  1:472375/1‑140 (MQ=255)
                        cGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAg                                                                                                               <  2:472375/140‑1 (MQ=255)
                          tgtATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCg                                                                                                             >  2:1187212/1‑140 (MQ=255)
                          tgtATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCg                                                                                                             >  2:883044/1‑140 (MQ=255)
                             aTCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGcc                                                                                                          <  2:990569/140‑1 (MQ=255)
                                tcGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTa                                                                                                       <  1:785112/140‑1 (MQ=255)
                                 cGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTaa                                                                                                      >  2:224250/1‑140 (MQ=255)
                                 cGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTaa                                                                                                      >  1:727875/1‑140 (MQ=255)
                                  gACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAAc                                                                                                     >  2:1187055/1‑140 (MQ=255)
                                  gACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAAc                                                                                                     >  2:75545/1‑140 (MQ=255)
                                  gACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAAc                                                                                                     >  1:70890/1‑140 (MQ=255)
                                  gACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAAc                                                                                                     >  1:108335/1‑140 (MQ=255)
                                      gTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGAtt                                                                                                 >  2:1390260/1‑140 (MQ=255)
                                        tCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTAc                                                                                               >  2:315886/1‑140 (MQ=255)
                                             gCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCAt                                                                                          <  1:224250/140‑1 (MQ=255)
                                             gCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCAt                                                                                          <  1:86210/140‑1 (MQ=255)
                                             gCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCAt                                                                                          <  1:1187055/140‑1 (MQ=255)
                                             gCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCAt                                                                                          <  1:975185/140‑1 (MQ=255)
                                             gCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCAt                                                                                          <  1:1187212/140‑1 (MQ=255)
                                             gCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCAt                                                                                          <  1:1390260/140‑1 (MQ=255)
                                                 gATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGc                                                                                      >  2:855554/1‑140 (MQ=255)
                                                 gATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGc                                                                                      >  1:795122/1‑140 (MQ=255)
                                                              attatCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTg                                                                         <  2:180511/140‑1 (MQ=255)
                                                               ttatCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGc                                                                        <  1:259875/140‑1 (MQ=255)
                                                                  tCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTCCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCgt                                                                     <  2:871936/140‑1 (MQ=255)
                                                                      gtggATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCgtgtgt                                                                 >  1:969521/1‑140 (MQ=255)
                                                                      gtggATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCgtgtgg                                                                 >  1:566645/1‑139 (MQ=255)
                                                                       tggATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGtgtgtg                                                                >  2:685655/1‑140 (MQ=255)
                                                                       tggATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGtgtgtg                                                                >  1:18736/1‑140 (MQ=255)
                                                                       tggATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGtgtgtg                                                                >  1:109560/1‑140 (MQ=255)
                                                                         gATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGAt                                                              >  2:269047/1‑140 (MQ=255)
                                                                                cTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGc                                                       >  2:1080769/1‑140 (MQ=255)
                                                                                cTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGc                                                       >  1:416204/1‑140 (MQ=255)
                                                                                        cagGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCcgcg                                               >  1:829296/1‑140 (MQ=255)
                                                                                        cagGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCcgcg                                               >  1:775507/1‑140 (MQ=255)
                                                                                         agGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGa                                              <  1:269047/140‑1 (MQ=255)
                                                                                          ggCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGAc                                             <  2:18736/140‑1 (MQ=255)
                                                                                             ggTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGt                                          >  2:1290120/1‑140 (MQ=255)
                                                                                                     tACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCg                                  <  2:795122/140‑1 (MQ=255)
                                                                                                         gCCGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTAcc                              >  1:1169997/1‑140 (MQ=255)
                                                                                                          ccGGAAGAAAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCg                             <  2:1028024/140‑1 (MQ=255)
                                                                                                                gaaAAACGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACg                       <  1:855554/140‑1 (MQ=255)
                                                                                                                 aaaaaCGTAAATTGCTGGTGCAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGa                      <  1:685655/140‑1 (MQ=255)
                                                                                                                                     cAATTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTg  <  1:1080769/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGCGTCTTATCAGCAGGCGGTGAATTTACTGCCGGAAGAAAAACGTAAATTGCTGGTGCAACTCTGAAAGGAAAAGGCCGCTCAGAAAGCGGCCTTAACGATTACAGCATCGGCTTAAGGAAGCGTGCCGTGTGTGATGCTTCGCACTCCGCGACGGTTTCTGGCGTACCGGAGACGAGGATCTCGCCGCCACCACTG  >  NC_000913/4270873‑4271145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A