Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,298,452	G→A	G305G (GGC→GGT)	fdhF ←	formate dehydrogenase H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,298,452	0	G	A	100.0%	236.5 / NA	70	G305G (GGC→GGT)	fdhF	formate dehydrogenase H
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (38/32); total (38/32)

TACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGT  >  NC_000913/4298315‑4298582

tACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACAcc                                                                                                                                  <  2:930975/140‑1 (MQ=255)

      tCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTa                                                                                                                            >  2:1404668/1‑140 (MQ=255)

           cAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACAccc                                                                                                                                 >  2:986398/1‑130 (MQ=255)

           cAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACAccc                                                                                                                                 <  1:986398/130‑1 (MQ=255)

           cAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACt                                                                                                                       <  2:317340/140‑1 (MQ=255)

           cAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACt                                                                                                                       <  2:1246323/140‑1 (MQ=255)

           cAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACt                                                                                                                       <  1:1355672/140‑1 (MQ=255)

           cAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACt                                                                                                                       <  2:657425/140‑1 (MQ=255)

           cAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACt                                                                                                                       >  2:91076/1‑140 (MQ=255)

             gcgcgcCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTgg                                                                                                                     >  1:1224703/1‑140 (MQ=255)

              cgcgcCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTggg                                                                                                                    >  1:1299284/1‑140 (MQ=255)

              cgcgcCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTggg                                                                                                                    >  2:706714/1‑140 (MQ=255)

                cgcCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTAGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGtt                                                                                                                  >  1:351785/1‑140 (MQ=255)

                   ccATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGAGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTaca                                                                                                               >  1:1299717/1‑140 (MQ=255)

                      tatCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACAccc                                                                                                            <  1:952151/140‑1 (MQ=255)

                         cGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATg                                                                                                         <  1:338894/140‑1 (MQ=255)

                          gCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGc                                                                                                        >  1:1403467/1‑140 (MQ=255)

                             ggCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGcccc                                                                                                     >  1:1085980/1‑140 (MQ=255)

                                 cccTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATg                                                                                                         >  1:371897/1‑132 (MQ=255)

                                 cccTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATg                                                                                                         <  2:371897/132‑1 (MQ=255)

                                 cccTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAg                                                                                                 >  1:1079478/1‑140 (MQ=255)

                                  ccTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAgg                                                                                                >  1:885702/1‑140 (MQ=255)

                                    tGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGAt                                                                                              >  1:176062/1‑140 (MQ=255)

                                          ttgttCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTCACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATggcggc                                                                                        >  2:1170691/1‑140 (MQ=255)

                                            gttCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGgcgc                                                                                      >  2:974183/1‑140 (MQ=255)

                                              tCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCtt                                                                                    <  1:759344/140‑1 (MQ=255)

                                                  aCCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCg                                                                                <  2:1079478/140‑1 (MQ=255)

                                                  aCCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCg                                                                                <  2:1085980/140‑1 (MQ=255)

                                                   ccACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGc                                                                               <  1:1129120/140‑1 (MQ=255)

                                                    cACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGcc                                                                              <  2:18905/140‑1 (MQ=255)

                                                    cACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGcc                                                                              <  1:974183/140‑1 (MQ=255)

                                                     aCGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCt                                                                             <  2:379025/140‑1 (MQ=255)

                                                     aCGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGAGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGTGCTTTTCGCCt                                                                             <  2:1299717/140‑1 (MQ=255)

                                                          ccGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCa                                                                        <  1:91076/140‑1 (MQ=255)

                                                          ccGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCa                                                                        <  2:1224703/140‑1 (MQ=255)

                                                            gggTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATa                                                                      >  1:599635/1‑140 (MQ=255)

                                                                    ccccGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTg                                                              <  2:161315/139‑1 (MQ=255)

                                                                       cGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTgccg                                                           >  1:209617/1‑140 (MQ=255)

                                                                       cGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTgccg                                                           >  1:151806/1‑140 (MQ=255)

                                                                       cGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTgccg                                                           >  1:472456/1‑140 (MQ=255)

                                                                       cGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTgccg                                                           >  1:641827/1‑140 (MQ=255)

                                                                        gCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTgccgc                                                          >  2:875239/1‑140 (MQ=255)

                                                                        gCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTgccgc                                                          >  2:703124/1‑140 (MQ=255)

                                                                         cATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTgccgcc                                                         >  1:927607/1‑140 (MQ=255)

                                                                           tGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTg                                                       <  2:209617/140‑1 (MQ=255)

                                                                                cTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGa                                                      <  1:550904/136‑1 (MQ=255)

                                                                                cTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGa                                                      >  2:550904/1‑136 (MQ=255)

                                                                                    ccGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAAtctc                                              >  2:718389/1‑140 (MQ=255)

                                                                                       aGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACt                                           <  2:885702/140‑1 (MQ=255)

                                                                                            aCCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGgcgc                                      >  2:761343/1‑140 (MQ=255)

                                                                                            aCCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGgcgc                                      >  1:877864/1‑140 (MQ=255)

                                                                                               ggTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGa                                   <  2:578903/140‑1 (MQ=255)

                                                                                                  cAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGc                                >  2:207795/1‑140 (MQ=255)

                                                                                                        cgcgAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGa                          <  1:761343/140‑1 (MQ=255)

                                                                                                         gcgAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGat                         <  1:207795/140‑1 (MQ=255)

                                                                                                          cgAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGata                        >  1:1109861/1‑140 (MQ=255)

                                                                                                           gAGGCTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGatat                       <  2:209153/140‑1 (MQ=255)

                                                                                                               cTGGTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTc                   >  2:46670/1‑140 (MQ=255)

                                                                                                                  gTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAAc                >  1:12871/1‑140 (MQ=255)

                                                                                                                  gTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAAc                >  2:737252/1‑140 (MQ=255)

                                                                                                                  gTCAGAGAACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAAc                >  1:373550/1‑140 (MQ=255)

                                                                                                                       agaACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACt           <  2:646738/140‑1 (MQ=255)

                                                                                                                       agaACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACt           <  1:875239/140‑1 (MQ=255)

                                                                                                                       agaACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACt           <  1:703124/140‑1 (MQ=255)

                                                                                                                        gaACGCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTc          <  2:641827/140‑1 (MQ=255)

                                                                                                                            gCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTcc         <  1:1329764/137‑1 (MQ=255)

                                                                                                                            gCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTcc         >  2:1329764/1‑137 (MQ=255)

                                                                                                                            gCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGc      >  2:540832/1‑140 (MQ=255)

                                                                                                                            gCACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGc      >  1:1109171/1‑140 (MQ=255)

                                                                                                                             cACGGTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCg     >  1:1344719/1‑140 (MQ=255)

                                                                                                                                ggTTTCCACACCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCgtgt  <  1:1404668/140‑1 (MQ=255)

TACGTATCCGGCAGCGCGCCCATATCGCAGGCACCCTGAACGTTGTTCTGACCACGAACCGGGTTAACACCCGCATGCGGCTTACCGAGGTTACCGGTCAGCATCGCGAGGCTGGTCAGAGAACGCACGGTTTCCACGCCCTGGTAGAACTGGGTTACACCCATGCCCCACAGGATGGCGGCGCTTTTCGCCTGGGCATACATCCGTGCCGCCTGACGAATCTCACTGGCGCTGACGCCGGTGATATCTTCAACCGACTCCGGCGTGT  >  NC_000913/4298315‑4298582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A