Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	159	82.1	2830106	99.3%	2810295	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,465,243	T→C	Y313C (TAC→TGC)	treB ←	trehalose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,465,243	0	T	C	100.0%	260.2 / NA	70	Y313C (TAC→TGC)	treB	trehalose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (46/24); total (46/24)

CTATCACGGCGGAGCCCTGAGCGATATTCGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGA  >  NC_000913/4465109‑4465378

cTATCACGGCGGAGCCCTGAGCGATATTCGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAgg                                                                                                                                    <  1:278779/140‑1 (MQ=255)

cTATCACGGCGGAGCCCTGAGCGATATTCGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAgg                                                                                                                                    <  2:455368/140‑1 (MQ=255)

       ggcggAGCCCTGAGCGATATTCGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCaa                                                                                                                             >  1:732456/1‑140 (MQ=255)

        gcggAGCCCTGAGCGATATTCGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCaaa                                                                                                                            >  2:514503/1‑140 (MQ=255)

                   aGCGATATTCGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTgcgc                                                                                                                 <  2:573768/140‑1 (MQ=255)

                   aGCGATATTCGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTgcgc                                                                                                                 >  2:747043/1‑140 (MQ=255)

                       atatTCGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAAt                                                                                                             <  2:913719/140‑1 (MQ=255)

                        tatTCGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATc                                                                                                            >  1:330126/1‑140 (MQ=255)

                                  cgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAAt                                                                                                             <  1:780237/129‑1 (MQ=255)

                                  cgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAAt                                                                                                             >  2:780237/1‑129 (MQ=255)

                                  cgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGc                                                                                                  >  2:529567/1‑140 (MQ=255)

                                  cgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGc                                                                                                  >  2:148895/1‑140 (MQ=255)

                                  cgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGc                                                                                                  >  1:184325/1‑140 (MQ=255)

                                     aaTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGc                                                                                               <  1:661377/140‑1 (MQ=255)

                                     aaTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGc                                                                                               >  2:1372317/1‑140 (MQ=255)

                                             gCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcatc                                                                                       <  2:732456/140‑1 (MQ=255)

                                                 cacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt                                                                                   >  2:306641/1‑140 (MQ=255)

                                                         tACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTAcc                                                                           >  2:424088/1‑140 (MQ=255)

                                                                cATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGt                                                                              >  1:125390/1‑130 (MQ=255)

                                                                cATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGt                                                                              <  2:125390/130‑1 (MQ=255)

                                                                cATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAgg                                                                    >  2:139294/1‑140 (MQ=255)

                                                                 aTGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc                                                                   >  2:1198878/1‑140 (MQ=255)

                                                                    cTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAAc                                                                >  2:1126146/1‑140 (MQ=255)

                                                                     tttGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACg                                                               >  1:188127/1‑140 (MQ=255)

                                                                         aaTCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCAt                                                           <  1:529567/140‑1 (MQ=255)

                                                                             atcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGcc                                                       >  1:844925/1‑140 (MQ=255)

                                                                              tcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCa                                                      >  1:561342/1‑140 (MQ=255)

                                                                                                gCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGa                                    >  1:17565/1‑140 (MQ=255)

                                                                                                gCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGa                                    <  1:139294/140‑1 (MQ=255)

                                                                                                  gTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGAcc                                  >  1:614594/1‑140 (MQ=255)

                                                                                                          gtgtACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGa                                    <  2:836285/130‑1 (MQ=255)

                                                                                                          gtgtACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGa                                    >  1:836285/1‑130 (MQ=255)

                                                                                                          gtgtACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAgcg                          >  2:494300/1‑140 (MQ=255)

                                                                                                          gggtACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAgcg                          >  2:549359/3‑140 (MQ=255)

                                                                                                           tgtACACCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAgcgc                         <  2:844925/140‑1 (MQ=255)

                                                                                                               cacCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCACTCGGAGCAATGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATgg                     <  2:1266083/140‑1 (MQ=255)

                                                                                                               cacCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATgg                     >  2:198864/1‑140 (MQ=255)

                                                                                                               cacCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATgg                     >  2:638206/1‑140 (MQ=255)

                                                                                                                accgcggATCACCAGCACGACGCACAGGAAGCCAAACAATGCTGCGCCCATCCGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCAGGCGACCAAACGGACCAATCAGCGCATggg                    >  2:1039669/7‑140 (MQ=255)

                                                                                                                acCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCAt                       <  2:99021/137‑1 (MQ=255)

                                                                                                                acCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCAt                       >  1:99021/1‑137 (MQ=255)

                                                                                                                acCGGTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATggg                    >  1:571331/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  2:458990/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  2:452031/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  2:273443/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  1:993535/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  1:861164/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  1:850791/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  1:75721/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  1:752847/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  1:592963/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  1:405825/1‑140 (MQ=255)

                                                                                                                    gTGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAg                >  1:28244/1‑140 (MQ=255)

                                                                                                                     tGATCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAgg               >  2:1073283/1‑140 (MQ=255)

                                                                                                                       aTCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGaa             >  2:1401033/1‑140 (MQ=255)

                                                                                                                       aTCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGaa             >  1:1102821/1‑140 (MQ=255)

                                                                                                                       aTCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGaa             <  1:452031/140‑1 (MQ=255)

                                                                                                                       aTCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGaa             <  2:614594/140‑1 (MQ=255)

                                                                                                                       aTCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGaa             <  1:705241/140‑1 (MQ=255)

                                                                                                                       aTCACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGaa             >  2:686840/1‑140 (MQ=255)

                                                                                                                         cACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAaca           <  2:861164/140‑1 (MQ=255)

                                                                                                                         cACCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAaca           >  1:251608/1‑140 (MQ=255)

                                                                                                                          aCCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAgcgc                         >  1:1178334/1‑125 (MQ=255)

                                                                                                                          aCCAGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAgcgc                         <  2:1178334/125‑1 (MQ=255)

                                                                                                                             aGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACcgc       <  1:747043/140‑1 (MQ=255)

                                                                                                                             aGCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACcgc       <  1:198864/140‑1 (MQ=255)

                                                                                                                              gCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACcgcg      <  1:458990/140‑1 (MQ=255)

                                                                                                                              gCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACcgcg      <  2:571331/140‑1 (MQ=255)

                                                                                                                              gCGGGGCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACcgcg      >  2:207339/1‑140 (MQ=255)

                                                                                                                                 gggCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAgg   >  2:707271/1‑140 (MQ=255)

                                                                                                                                  ggCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGa  >  1:220665/1‑140 (MQ=255)

                                                                                                                                  ggCGCACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGa  <  1:1198878/140‑1 (MQ=255)

CTATCACGGCGGAGCCCTGAGCGATATTCGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCATGCGACCAAACGGACCAATCAGCGCATGGGCGAGGAACACCGCGAGGA  >  NC_000913/4465109‑4465378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A