Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,675	A→G	intergenic (‑149/+46)	fnr ← / ← ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,675	0	A	G	100.0%	173.9 / NA	49	intergenic (‑149/+46)	fnr/ogt	DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/21); total (28/21)

GCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGCCA  >  NC_000913/1399539‑1399808

gCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACt                                                                                                                                    >  2:21434/1‑140 (MQ=255)

gCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACt                                                                                                                                    >  1:130362/1‑140 (MQ=255)

        tGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATaa                                                                                                                            <  2:130362/140‑1 (MQ=255)

            atTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAgt                                                                                                                        <  1:21434/140‑1 (MQ=255)

                 tCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGaa                                                                                                                   <  2:611655/140‑1 (MQ=255)

                            aTCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGcac                                                                                                        <  1:193395/140‑1 (MQ=255)

                                        aGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACaa                                                                                                      <  2:836502/130‑1 (MQ=255)

                                        aGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACaa                                                                                                      >  1:836502/1‑130 (MQ=255)

                                         gCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATg                                                                                           >  1:823079/1‑140 (MQ=255)

                                         gCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATg                                                                                           >  1:386015/1‑140 (MQ=255)

                                           aagtaagtCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGtt                                                                                         <  2:432171/140‑1 (MQ=255)

                                            agtaagtCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGttt                                                                                        <  2:386015/140‑1 (MQ=255)

                                              taagtCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTAc                                                                                      <  2:273346/140‑1 (MQ=255)

                                                  tCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCa                                                                                  >  1:440191/1‑140 (MQ=255)

                                                     aaGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCaaaa                                                                               >  1:1082596/1‑140 (MQ=255)

                                                       gCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGa                                                                             >  2:177451/1‑140 (MQ=255)

                                                            ccTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTa                                                                                       >  1:624781/1‑125 (MQ=255)

                                                            ccTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTa                                                                                       <  2:624781/125‑1 (MQ=255)

                                                            ccTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAAcc                                                                        >  2:141088/1‑140 (MQ=255)

                                                                aaCAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCa                                                                    >  2:282349/1‑140 (MQ=255)

                                                                    gcagAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGgc                                                                <  2:390219/140‑1 (MQ=255)

                                                                                  tatTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCActct                                                  <  1:141088/140‑1 (MQ=255)

                                                                                  tatTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCActct                                                  <  2:947925/140‑1 (MQ=255)

                                                                                  tatTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCActct                                                  <  2:440191/140‑1 (MQ=255)

                                                                                    ttAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCttt                                                >  1:451634/1‑140 (MQ=255)

                                                                                    ttAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCttt                                                <  2:48214/140‑1 (MQ=255)

                                                                                        aTGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGc                                            <  2:142976/140‑1 (MQ=255)

                                                                                            aaaTATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGaa                                        >  2:1070175/1‑140 (MQ=255)

                                                                                                  atGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGc                                  >  1:205686/1‑140 (MQ=255)

                                                                                                   tGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGcc                                 >  1:830271/1‑140 (MQ=255)

                                                                                                   tGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGcc                                 <  1:1070175/140‑1 (MQ=255)

                                                                                                       tatctatTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCa                             >  1:1058116/1‑140 (MQ=255)

                                                                                                         tctatTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                           >  2:985042/1‑140 (MQ=255)

                                                                                                         tctatTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                           >  1:351429/1‑140 (MQ=255)

                                                                                                         tctatTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCata                           <  1:177451/140‑1 (MQ=255)

                                                                                                          ctatTTGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCatat                          <  2:205686/140‑1 (MQ=255)

                                                                                                             tttGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCg                       >  1:1014882/1‑140 (MQ=255)

                                                                                                              ttGAAAACCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCgg                      <  1:859457/140‑1 (MQ=255)

                                                                                                                  aaaCCCTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCAt                  <  2:830271/140‑1 (MQ=255)

                                                                                                                     cccTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCAt                  <  2:1077826/137‑1 (MQ=255)

                                                                                                                     cccTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCAt                  >  1:1077826/1‑137 (MQ=255)

                                                                                                                       cTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGc             >  2:565953/1‑140 (MQ=255)

                                                                                                                       cTTAAGTTGTTAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGc             >  1:263948/1‑140 (MQ=255)

                                                                                                                              tgttAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCg      <  2:797239/140‑1 (MQ=255)

                                                                                                                                ttAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAGAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGc    >  2:1009890/1‑140 (MQ=255)

                                                                                                                                ttAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGc    >  1:167388/1‑140 (MQ=255)

                                                                                                                                ttAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGc    >  2:715168/1‑140 (MQ=255)

                                                                                                                                ttAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGc    >  1:432730/1‑140 (MQ=255)

                                                                                                                                ttAAGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGc    >  1:744258/1‑140 (MQ=255)

                                                                                                                                  aaGGGTGACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGCCa  >  2:534652/1‑140 (MQ=255)

GCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAGCAAACCTTAACAGCAGAGAATTCCGATATTAGATGTAAATATATGTCTATCTATTTGAAAACCCTTAAGTTGTTAAGGGTAACTTTACATAAAAGTGTGAACAAGCTGGCACAAATTGTTTAATGTTTACAGCAAAAGATAACCTTCATGGCGCAATAACCACTCTTTTCGCTGAACTCCGCCTGCATATCCGGTCATGGTGCCGTTTCGGCCA  >  NC_000913/1399539‑1399808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A