Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,403,605 C→T G321S (GGC→AGC)  abgA ← p‑aminobenzoyl‑glutamate hydrolase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,403,6050CT100.0% 198.1 / NA 58G321S (GGC→AGC) abgAp‑aminobenzoyl‑glutamate hydrolase subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/32);  total (26/32)

CAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAACGCCCACGTTTA  >  NC_000913/1403470‑1403739
                                                                                                                                       |                                                                                                                                      
cAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGa                                                                                                                                    <  1:762117/140‑1 (MQ=255)
  aTGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAAt                                                                                                                                  <  1:1159961/140‑1 (MQ=255)
    ggCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCg                                                                                                                                <  2:922727/140‑1 (MQ=255)
     gCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGc                                                                                                                               <  1:121752/140‑1 (MQ=255)
     gCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGc                                                                                                                               <  1:792019/140‑1 (MQ=255)
       cTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCtt                                                                                                                             >  2:330599/1‑140 (MQ=255)
          aTTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATc                                                                                                                                 >  2:542064/1‑133 (MQ=255)
          aTTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATc                                                                                                                                 <  1:542064/133‑1 (MQ=255)
               cccccGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCa                                                                                                                     <  2:108126/140‑1 (MQ=255)
               cccccGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCa                                                                                                                     <  2:868218/140‑1 (MQ=255)
               cccccGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCa                                                                                                                     <  1:140068/140‑1 (MQ=255)
               cccccGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCa                                                                                                                     <  1:330599/140‑1 (MQ=255)
                ccccGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCTTACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCAc                                                                                                                    <  2:1109245/140‑1 (MQ=255)
                ccccGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCAc                                                                                                                    >  2:182657/1‑140 (MQ=255)
                   cgcgACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGt                                                                                                                 >  1:720893/1‑140 (MQ=255)
                   cgcgACCTGAGCCGCCTAACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGt                                                                                                                 <  2:606166/140‑1 (MQ=255)
                    gcgACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTc                                                                                                                >  1:144357/1‑140 (MQ=255)
                            aGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACat                                                                                                        >  2:46142/1‑140 (MQ=255)
                             gccgccTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACata                                                                                                       <  1:321592/140‑1 (MQ=255)
                             gccgccTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACata                                                                                                       <  1:1235231/140‑1 (MQ=255)
                                                aTGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTg                                                                                    >  2:25803/1‑140 (MQ=255)
                                                      cccATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCcg                                                                              <  1:46142/140‑1 (MQ=255)
                                                           tGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCgtgt                                                                         >  1:863650/1‑140 (MQ=255)
                                                           tGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGGTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCgtgt                                                                         >  1:473032/1‑140 (MQ=255)
                                                            gcggcgAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCcgcg                                                                            <  1:52893/136‑1 (MQ=255)
                                                            gcggcgAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCcgcg                                                                            >  2:52893/1‑136 (MQ=255)
                                                                            ggCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACg                                                        <  1:42666/140‑1 (MQ=255)
                                                                             gCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGc                                                       >  2:544870/1‑140 (MQ=255)
                                                                                   gCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGc                                                 <  2:397688/140‑1 (MQ=255)
                                                                                       cACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGa                                             >  2:1052362/1‑140 (MQ=255)
                                                                                       cACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGa                                             <  2:144357/140‑1 (MQ=255)
                                                                                            aTCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGaacaac                                        >  2:564631/1‑140 (MQ=255)
                                                                                             tCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGaac                                           <  2:318409/136‑1 (MQ=255)
                                                                                             tCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGaac                                           >  1:318409/1‑136 (MQ=255)
                                                                                                gACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTa                                    <  1:1220816/140‑1 (MQ=255)
                                                                                                    aGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGAc                                >  2:626277/1‑140 (MQ=255)
                                                                                                    aGTTTCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGAc                                <  1:544870/140‑1 (MQ=255)
                                                                                                        tCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGa                                 <  2:729286/135‑1 (MQ=255)
                                                                                                        tCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGa                                 >  1:729286/1‑135 (MQ=255)
                                                                                                        tCAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCt                            >  2:590213/1‑140 (MQ=255)
                                                                                                         cAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCtt                           <  1:542784/140‑1 (MQ=255)
                                                                                                         cAACGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCtt                           <  1:182657/140‑1 (MQ=255)
                                                                                                           aCGCCGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTcc                         >  2:613049/1‑140 (MQ=255)
                                                                                                              ccGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTcctgc                      <  1:1052362/140‑1 (MQ=255)
                                                                                                               cGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTcctgcc                     <  1:599693/140‑1 (MQ=255)
                                                                                                               cGACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAAAGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTcctgcc                     <  1:25803/140‑1 (MQ=255)
                                                                                                                gACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGAcc                               <  2:632134/129‑1 (MQ=255)
                                                                                                                gACACCATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGAcc                               >  1:632134/1‑129 (MQ=255)
                                                                                                                     cATACATGGTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATa               <  2:720893/140‑1 (MQ=255)
                                                                                                                            ggTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTcctgcct                    >  2:969536/1‑128 (MQ=255)
                                                                                                                            ggTTGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTcctgcct                    <  1:969536/128‑1 (MQ=255)
                                                                                                                              ttGCTGCGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAACGCCCACg      >  2:740675/1‑140 (MQ=255)
                                                                                                                               tgctgcGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAACGc           >  2:636922/1‑134 (MQ=255)
                                                                                                                               tgctgcGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAACGc           <  1:636922/134‑1 (MQ=255)
                                                                                                                               tgctgcGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAACGCCCACGt     >  2:1255470/1‑140 (MQ=255)
                                                                                                                                 ctgcGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAACGCCCACGttt   >  2:430835/1‑140 (MQ=255)
                                                                                                                                 ctgcGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAACGCCCACGttt   >  2:1060483/1‑140 (MQ=255)
                                                                                                                                 ctgcGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAACGCCCACGttt   >  1:945497/1‑140 (MQ=255)
                                                                                                                                  tgcGCTCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAACGCCCACGTTTa  <  1:1255470/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACCCATCAGACGAGTTTCAACGCCGACACCATACATGGTTGCTGCGCCCTGAATCGCTTGTTGTGCACGGTCAAAAACATATTGATTAATGACGTCGCTGGCCCCGCGTGTTTCCACTTTCAGCAACGCCGAGGCAGGAACAACGTTACGACCGCTTCCTGCCTGCATAACGCCCACGTTTA  >  NC_000913/1403470‑1403739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A