Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,580,210	G→A	P194L (CCG→CTG)	ydeM ←	putative anaerobic sulfatase maturation enzyme YdeM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,580,210	0	G	A	100.0%	171.1 / NA	49	P194L (CCG→CTG)	ydeM	putative anaerobic sulfatase maturation enzyme YdeM
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/27); total (22/27)

CGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACT  >  NC_000913/1580094‑1580338

cGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTca                                                                                                           <  2:170705/140‑2 (MQ=255)

   ttttAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATa                                                                                                        <  2:658228/140‑1 (MQ=255)

   ttttAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATa                                                                                                        <  1:829365/140‑1 (MQ=255)

        aCCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTg                                                                                                   >  1:386769/1‑140 (MQ=255)

            aTTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCata                                                                                               >  1:915208/1‑140 (MQ=255)

             ttGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATaa                                                                                                       >  2:724802/1‑131 (MQ=255)

             ttGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATaa                                                                                                       <  1:724802/131‑1 (MQ=255)

                 aTAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGttt                                                                                          <  1:442082/140‑1 (MQ=255)

                 aTAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGttt                                                                                          <  1:74612/140‑1 (MQ=255)

                        tGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCg                                                                                   >  1:121015/1‑140 (MQ=255)

                          gTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTa                                                                                         >  2:342437/1‑132 (MQ=255)

                          gTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTa                                                                                         <  1:342437/132‑1 (MQ=255)

                          gTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCAGTTTCTAGCAATTCGATAAATTGCATATGTTTa                                                                                         >  1:1040304/1‑132 (MQ=255)

                          gTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCAGTTTCTAGCAATTCGATAAATTGCATATGTTTa                                                                                         <  2:1040304/132‑1 (MQ=255)

                                        gCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAAt                                                                   >  1:515995/1‑140 (MQ=255)

                                        gCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAAt                                                                   >  2:743878/1‑140 (MQ=255)

                                        gCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAAt                                                                   >  1:398876/1‑140 (MQ=255)

                                        gCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAAt                                                                   >  2:1048952/1‑140 (MQ=255)

                                          cATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGa                                                                 <  1:1020394/140‑1 (MQ=255)

                                           aTAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGAt                                                                <  2:121015/140‑1 (MQ=255)

                                            tAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATa                                                               <  2:627012/140‑1 (MQ=255)

                                                   tGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCa                                                        >  2:458022/1‑140 (MQ=255)

                                                    gggAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCaa                                                       <  1:743878/140‑1 (MQ=255)

                                                        ggCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCaa                                                       <  2:352246/136‑1 (MQ=255)

                                                        ggCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCaa                                                       >  1:352246/1‑136 (MQ=255)

                                                             aGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAAt                                              >  1:1056704/1‑140 (MQ=255)

                                                                    tCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACAtt                                       <  2:915208/140‑1 (MQ=255)

                                                                    tCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACAtt                                       <  2:515995/140‑1 (MQ=255)

                                                                        tGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACg                                   <  2:386769/140‑1 (MQ=255)

                                                                                aaTGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAAt                           <  2:368443/140‑1 (MQ=255)

                                                                                 aTGTGTTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATg                          <  2:329433/140‑1 (MQ=255)

                                                                                   gtgtTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGAc                        <  2:425252/140‑1 (MQ=255)

                                                                                    tgtTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACg                       >  2:448830/1‑140 (MQ=255)

                                                                                     gtTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAGAAAATGATAAACCTCAAGAGGGTAATGGACATTAACg                                   >  1:317546/1‑127 (MQ=255)

                                                                                     gtTCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAGAAAATGATAAACCTCAAGAGGGTAATGGACATTAACg                                   <  2:317546/127‑1 (MQ=255)

                                                                                      ttCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACAtt                                       <  1:276136/122‑1 (MQ=255)

                                                                                      ttCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACAtt                                       >  2:276136/1‑122 (MQ=255)

                                                                                      ttCTCACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGt                     >  1:47122/1‑140 (MQ=255)

                                                                                         tcACTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTaa                  <  1:458022/140‑1 (MQ=255)

                                                                                            cTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTa                   <  2:582430/136‑1 (MQ=255)

                                                                                            cTATGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTa                   >  1:582430/1‑136 (MQ=255)

                                                                                              aTGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTAt             >  1:414836/1‑140 (MQ=255)

                                                                                              aTGACCACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTAt             >  1:719682/1‑140 (MQ=255)

                                                                                                  ccACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTaaa         <  1:448830/140‑1 (MQ=255)

                                                                                                   cACTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAAc        >  1:6225/1‑140 (MQ=255)

                                                                                                     cTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAActc      >  1:995346/1‑140 (MQ=255)

                                                                                                     cTGAAATCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAActc      <  2:47122/140‑1 (MQ=255)

                                                                                                         aaTCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACt  <  2:398876/140‑1 (MQ=255)

                                                                                                         aaTCAATATTCAGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACt  <  1:516652/140‑1 (MQ=255)

CGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACT  >  NC_000913/1580094‑1580338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A