Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,674,901	G→A	G330E (GGA→GAA)	tqsA →	autoinducer 2 exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,674,901	0	G	A	100.0%	180.3 / NA	51	G330E (GGA→GAA)	tqsA	autoinducer 2 exporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/30); total (21/30)

GGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTT  >  NC_000913/1674766‑1675036

ggCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGt                                                                                                                                     <  1:1058208/140‑1 (MQ=255)

ggCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGt                                                                                                                                     >  2:162565/1‑140 (MQ=255)

  cGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCa                                                                                                                                   <  1:706258/140‑1 (MQ=255)

  cGTGGGATGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCa                                                                                                                                   >  2:722974/1‑140 (MQ=255)

   gTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCaa                                                                                                                                  <  2:357295/140‑1 (MQ=255)

   gTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCaa                                                                                                                                  >  1:357295/1‑140 (MQ=255)

    tGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCaaa                                                                                                                                 <  1:757998/140‑1 (MQ=255)

    tGGGCTGGGGCTTTCCACATTGGTGGCATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCaaa                                                                                                                                 <  1:791845/140‑1 (MQ=255)

      ggCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGc                                                                                                                               <  1:1022282/140‑1 (MQ=255)

      ggCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGc                                                                                                                               >  2:736396/1‑140 (MQ=255)

      ggCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGc                                                                                                                               >  2:348953/1‑140 (MQ=255)

      ggCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGc                                                                                                                               >  2:887850/1‑140 (MQ=255)

              cTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGt                                                                                                                       <  1:348953/140‑1 (MQ=255)

               tttCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGtt                                                                                                                      <  1:736396/140‑1 (MQ=255)

               tttCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGtt                                                                                                                      <  1:887850/140‑1 (MQ=255)

                 tCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGtt                                                                                                                      <  2:767199/138‑1 (MQ=255)

                 tCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGtt                                                                                                                      >  1:767199/1‑138 (MQ=255)

                 tCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCt                                                                                                                    >  2:346843/1‑140 (MQ=255)

                 tCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCt                                                                                                                    >  1:1021287/1‑140 (MQ=255)

                      aTTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTaa                                                                                                               <  1:40102/140‑1 (MQ=255)

                        tggtggTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGc                                                                                                             <  2:1021287/140‑1 (MQ=255)

                          gtggtATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGa                                                                                                           <  1:346843/140‑1 (MQ=255)

                            ggtATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGAtc                                                                                                         <  2:503503/140‑1 (MQ=255)

                            ggtATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGAtc                                                                                                         <  1:1068898/140‑1 (MQ=255)

                             gtATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGAtct                                                                                                        >  1:583209/1‑140 (MQ=255)

                                tttttGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCaa                                                                                                     <  2:1127693/140‑1 (MQ=255)

                                tttttGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCaa                                                                                                     <  1:570645/140‑1 (MQ=255)

                                tttttGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCaa                                                                                                     >  1:757751/1‑140 (MQ=255)

                                tttttGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCaa                                                                                                     <  2:757751/140‑1 (MQ=255)

                                     gTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGAGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAag                                                                                                >  2:876569/1‑140 (MQ=255)

                                           gATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGAc                                                                                          <  2:320062/140‑1 (MQ=255)

                                           gATTTTTTGGGGATGGTTGTTAGGACCGGAGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGAc                                                                                          <  1:876569/140‑1 (MQ=255)

                                                             gttAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGt                                                                        <  1:251559/140‑1 (MQ=255)

                                                             gttAGCACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACACTTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGt                                                                        <  2:1000744/140‑1 (MQ=255)

                                                                 ggACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAgg                                                                    >  2:79160/1‑140 (MQ=255)

                                                                   aCCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGt                                                                        <  1:832005/134‑1 (MQ=255)

                                                                   aCCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGt                                                                        >  2:832005/1‑134 (MQ=255)

                                                                   aCCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGt                                                                  >  1:967130/1‑140 (MQ=255)

                                                                    ccGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGtt                                                                 >  2:740078/1‑140 (MQ=255)

                                                                     cGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                >  1:574352/1‑140 (MQ=255)

                                                                     cGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTa                                                                >  1:851411/1‑140 (MQ=255)

                                                                       gTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACa                                                              <  1:888208/140‑1 (MQ=255)

                                                                       gTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACa                                                              <  2:699039/140‑1 (MQ=255)

                                                                         gggTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACaga                                                            <  2:498524/140‑1 (MQ=255)

                                                                         gggTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACaga                                                            <  1:722974/140‑1 (MQ=255)

                                                                               gctgctTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTc                                                      >  2:1003990/1‑140 (MQ=255)

                                                                                        cGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCa                                             >  2:72424/1‑140 (MQ=255)

                                                                                                        attGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCATGATTGCATCCACGCTGGCTTTgg                             <  1:79160/140‑1 (MQ=255)

                                                                                                          tGTCAAAATTGCGCTTGAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCg                           <  1:740078/140‑1 (MQ=255)

                                                                                                                          gAACAAACAGCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgt           >  2:477193/1‑140 (MQ=255)

                                                                                                                                   gCGGAAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGtttt  <  2:967130/140‑1 (MQ=255)

GGCGTGGGCTGGGGCTTTCCACATTGGTGGTATTTTTGTCGTTGATTTTTTGGGGATGGTTGTTAGGACCGGTGGGTATGCTGCTTTCCGTGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTT  >  NC_000913/1674766‑1675036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A