Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,703,538 T→C E257E (GAA→GAG)  ydgJ ← putative oxidoreductase YdgJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,703,5380TC86.1% 225.7 / 21.8 87E257E (GAA→GAG) ydgJputative oxidoreductase YdgJ
Reads supporting (aligned to +/- strand):  ref base T (5/7);  new base C (35/39);  total (40/47)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCG  >  NC_000913/1703403‑1703670
                                                                                                                                       |                                                                                                                                    
gTAAGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGctctt                                                                                                                                  <  2:783059/140‑1 (MQ=255)
gTAAGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGctctt                                                                                                                                  <  1:752357/140‑1 (MQ=255)
   aGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCt                                                                                                                               >  1:532878/1‑140 (MQ=255)
   aGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCt                                                                                                                               >  1:185422/1‑140 (MQ=255)
      cGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCtgtg                                                                                                                            >  2:540720/1‑140 (MQ=255)
        gATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTggt                                                                                                                          >  1:584291/1‑139 (MQ=255)
           aaTTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACG‑‑‑TTCCTGTGGATCGAgg                                                                                                                    <  2:733329/140‑1 (MQ=255)
              tCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACGTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAgg                                                                                                                    <  2:1046536/140‑1 (MQ=255)
              tCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAgg                                                                                                                    <  1:280309/140‑1 (MQ=255)
              tCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAgg                                                                                                                    <  1:657647/140‑1 (MQ=255)
                   ggCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCata                                                                                                               >  2:797282/1‑140 (MQ=255)
                        cGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTca                                                                                                          >  1:593773/1‑140 (MQ=255)
                        cGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTca                                                                                                          >  2:930749/1‑140 (MQ=255)
                          tCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAgg                                                                                                                    >  1:715545/1‑128 (MQ=255)
                          tCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAgg                                                                                                                    <  2:715545/128‑1 (MQ=255)
                          tCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTcaca                                                                                                        >  1:453739/1‑140 (MQ=255)
                          tCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTcaca                                                                                                        >  2:539115/1‑140 (MQ=255)
                             aCAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATaa                                                                                                     <  2:185422/140‑1 (MQ=255)
                             aCAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATaa                                                                                                     <  2:532878/140‑1 (MQ=255)
                              cAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATaa                                                                                                     <  1:872138/139‑1 (MQ=255)
                              cAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATaa                                                                                                     >  2:872138/1‑139 (MQ=255)
                               aGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACt                                                                                                   <  1:930749/140‑1 (MQ=255)
                               aGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACt                                                                                                   <  1:1075509/140‑1 (MQ=255)
                                  gTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGcc                                                                                                <  2:411650/140‑1 (MQ=255)
                                  gTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGcc                                                                                                >  1:799512/1‑140 (MQ=255)
                                  gTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGcc                                                                                                >  2:1068538/1‑140 (MQ=255)
                                    ttcttcGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTc                                                                                              >  1:487053/1‑140 (MQ=255)
                                         cGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGAt                                                                                         <  1:738580/140‑1 (MQ=255)
                                           acacGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATcc                                                                                       >  2:745254/1‑140 (MQ=255)
                                           acacGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATcc                                                                                       <  1:797282/140‑1 (MQ=255)
                                           acacGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATcc                                                                                       <  2:799512/140‑1 (MQ=255)
                                             acGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTCCACATAACTGCCTCGGGATCCAt                                                                                     <  1:168270/140‑1 (MQ=255)
                                              cGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATg                                                                                    <  2:487053/140‑1 (MQ=255)
                                              cGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATg                                                                                    <  1:228824/140‑1 (MQ=255)
                                               gTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGc                                                                                   <  1:251289/140‑1 (MQ=255)
                                               gTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGc                                                                                   <  1:716977/140‑1 (MQ=255)
                                               gTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGc                                                                                   <  1:62614/140‑1 (MQ=255)
                                                ttCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGc                                                                                   <  2:461843/139‑1 (MQ=255)
                                                ttCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGc                                                                                   >  1:1129512/1‑139 (MQ=255)
                                                ttCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGc                                                                                   <  2:1129512/139‑1 (MQ=255)
                                                ttCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGc                                                                                   >  1:461843/1‑139 (MQ=255)
                                                 tCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACt                                                                                                   >  1:607594/1‑122 (MQ=255)
                                                 tCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACt                                                                                                   <  2:607594/122‑1 (MQ=255)
                                                           ccACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGt                                                                       >  1:447110/1‑140 (MQ=255)
                                                             aCGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGc                                                                     >  1:487413/1‑140 (MQ=255)
                                                                  ggTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACt                                                                >  1:152810/1‑140 (MQ=255)
                                                                          cGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCt                                                        <  2:51408/140‑1 (MQ=255)
                                                                           gCCATCACACATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTg                                                       >  2:623437/1‑140 (MQ=255)
                                                                            ccATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                                      <  2:409385/140‑1 (MQ=255)
                                                                            ccATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                                      <  2:1093155/140‑1 (MQ=255)
                                                                            ccATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                                      <  1:539115/140‑1 (MQ=255)
                                                                            ccATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                                      <  1:154692/140‑1 (MQ=255)
                                                                             cATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGcc                                                     >  1:609778/1‑140 (MQ=255)
                                                                             cATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGcc                                                     >  1:614572/1‑140 (MQ=255)
                                                                              aTCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCa                                                    <  1:745254/140‑1 (MQ=255)
                                                                                         cGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCg                                         >  2:327935/1‑140 (MQ=255)
                                                                                             gCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTa                                     >  2:327645/1‑140 (MQ=255)
                                                                                              ccccAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTaa                                    >  1:500838/1‑140 (MQ=255)
                                                                                                 cAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAAt                                 >  2:36523/1‑140 (MQ=255)
                                                                                                  aGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATg                                >  2:773356/1‑140 (MQ=255)
                                                                                                  aGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATg                                               >  1:22189/1‑125 (MQ=255)
                                                                                                  aGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATg                                               <  2:22189/125‑1 (MQ=255)
                                                                                                   gTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGa                               >  1:577046/1‑140 (MQ=255)
                                                                                                   gTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGa                               >  2:184269/1‑140 (MQ=255)
                                                                                                   gTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGa                               >  2:353305/1‑140 (MQ=255)
                                                                                                   gTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGa                               >  2:1003519/1‑140 (MQ=255)
                                                                                                   gTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGa                               >  2:885230/1‑140 (MQ=255)
                                                                                                    tCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTa                                            >  2:226838/1‑126 (MQ=255)
                                                                                                    tCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTa                                            <  1:226838/126‑1 (MQ=255)
                                                                                                       tCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcg                           <  2:1078344/140‑1 (MQ=255)
                                                                                                        ccTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcg                           >  2:113384/1‑139 (MQ=255)
                                                                                                        ccTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcg                           <  1:113384/139‑1 (MQ=255)
                                                                                                        ccTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGCACCGTGTAAAATGACTcgc                          >  2:162919/1‑140 (MQ=255)
                                                                                                          tGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcgccg                        <  2:593773/140‑1 (MQ=255)
                                                                                                            cGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCt                      <  1:885230/140‑1 (MQ=255)
                                                                                                            cGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCt                      <  1:1003519/140‑1 (MQ=255)
                                                                                                            cGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCt                      <  2:614572/140‑1 (MQ=255)
                                                                                                            cGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCt                      <  1:353305/140‑1 (MQ=255)
                                                                                                            cGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCt                      <  1:623437/140‑1 (MQ=255)
                                                                                                            cGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCt                      <  2:152810/140‑1 (MQ=255)
                                                                                                            cGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCt                      <  2:487413/140‑1 (MQ=255)
                                                                                                              gTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCtgt                    >  1:915382/1‑140 (MQ=255)
                                                                                                                 gACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGa                 <  2:447110/140‑1 (MQ=255)
                                                                                                                 gACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGa                 <  1:36523/140‑1 (MQ=255)
                                                                                                                        gCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACa          >  2:730646/1‑140 (MQ=255)
                                                                                                                        gCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACa          <  2:609778/140‑1 (MQ=255)
                                                                                                                               ttCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGc   <  1:773356/140‑1 (MQ=255)
                                                                                                                               ttCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGc   <  1:1068538/140‑1 (MQ=255)
                                                                                                                                tCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCg  <  1:730646/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GTAAGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTAGCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGACAAGATGGCG  >  NC_000913/1703403‑1703670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A