Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,741,746 G→A A112T (GCC→ACC)  cfa → cyclopropane fatty acyl phospholipid synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,741,7460GA100.0% 148.3 / NA 44A112T (GCC→ACC) cfacyclopropane fatty acyl phospholipid synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

TGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGC  >  NC_000913/1741611‑1741873
                                                                                                                                       |                                                                                                                               
tggGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTg                                                                                                                             <  1:360050/140‑1 (MQ=255)
          aCCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGc                                                                                                                   >  2:77999/1‑140 (MQ=255)
              aCTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAag                                                                                                               >  2:606795/1‑140 (MQ=255)
              aCTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTC‑GCAAaga                                                                                                              >  2:889283/1‑140 (MQ=255)
               cTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAaga                                                                                                              >  1:909734/1‑140 (MQ=255)
                tGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAagag                                                                                                             <  1:188183/140‑1 (MQ=255)
                    tatGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCAtt                                                                                                         <  2:909734/140‑1 (MQ=255)
                               gCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGt                                                                                              <  1:606795/140‑1 (MQ=255)
                                cAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCAtt                                                                                                         <  2:65054/128‑1 (MQ=255)
                                cAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCAtt                                                                                                         >  1:65054/1‑128 (MQ=255)
                                    gTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGa                                                                                         <  2:530453/140‑1 (MQ=255)
                                              cAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGc                                                                               >  1:513589/1‑140 (MQ=255)
                                               aGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGcc                                                                              >  1:693581/1‑140 (MQ=255)
                                                ggTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCg                                                                             <  1:1108221/140‑1 (MQ=255)
                                                 gTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGc                                                                            <  2:513589/140‑1 (MQ=255)
                                                  tctcGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCa                                                                           <  2:322085/140‑1 (MQ=255)
                                                       agaACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCtt                                                                      >  1:338410/1‑140 (MQ=255)
                                                        gaACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTg                                                                     <  2:923133/140‑1 (MQ=255)
                                                         aaCCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGa                                                                    >  2:865354/1‑140 (MQ=255)
                                                           ccAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGc                                                                        <  1:1056021/134‑1 (MQ=255)
                                                           ccAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGc                                                                        >  2:1056021/1‑134 (MQ=255)
                                                           ccAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATc                                                                  >  2:55192/1‑140 (MQ=255)
                                                              aCTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCt                                                               >  2:194234/1‑140 (MQ=255)
                                                                   cccATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATg                                                          >  2:865763/1‑140 (MQ=255)
                                                                      atcatTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCaa                                                       <  2:671046/140‑1 (MQ=255)
                                                                      atcatTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCaa                                                       <  1:282496/140‑1 (MQ=255)
                                                                      atcatTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGCTCAGCCGCATGCTTGATCCCTTCATGCaa                                                       >  2:131125/1‑140 (MQ=255)
                                                                                aGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTgcg                                             <  1:55192/140‑1 (MQ=255)
                                                                                aGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTgcg                                             >  1:241346/1‑140 (MQ=255)
                                                                                aGACACGCTGCGTATTGCCGGCGCTCGTCTATTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTgcg                                             <  2:693581/140‑1 (MQ=255)
                                                                                   cacGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCtt                                          >  1:698556/1‑140 (MQ=255)
                                                                                     cGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTAc                                        >  2:207171/1‑140 (MQ=255)
                                                                                         gCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGa                                    <  1:207171/140‑1 (MQ=255)
                                                                                            tATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAg                                 >  2:601950/1‑140 (MQ=255)
                                                                                                  cggggttCGTCTCTTAAATCTGCAGGGTAAAAACCGTCCTGGGATAG‑CCGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGa                          <  1:889283/134‑1 (MQ=255)
                                                                                                  cGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGCTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCg                           <  1:131125/140‑1 (MQ=255)
                                                                                                    gcgcTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCt                                           <  2:114714/122‑1 (MQ=255)
                                                                                                    gcgcTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCt                                           >  1:114714/1‑122 (MQ=255)
                                                                                                    gcgcTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGaaa                                  >  2:755215/1‑131 (MQ=255)
                                                                                                    gcgcTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGaaa                                  <  1:755215/131‑1 (MQ=255)
                                                                                                      gcTCGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATaa                       >  1:507222/1‑140 (MQ=255)
                                                                                                         cGTCTCTTCAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCt                    >  2:850438/1‑140 (MQ=255)
                                                                                                                 cAATCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTg            <  2:698556/140‑1 (MQ=255)
                                                                                                                    tCTGCAGAGTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGccc         <  1:77999/140‑1 (MQ=255)
                                                                                                                           agTAAAAAACGTACCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGc  >  2:332383/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                               
TGGGAATGTGACCGACTGGATATGTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGCATTACGATTTGGGTAATGACTTGTTCAGCCGCATGCTTGATCCCTTCATGCAATATTCCTGCGCTTACTGGAAAGATGCCGATAATCTGGAATCTGCCCAGCAGGC  >  NC_000913/1741611‑1741873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A