Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,037,311	T→C	E281G (GAG→GGG)	hprS ←	sensory histidine kinase HprS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,037,311	0	T	C	100.0%	195.5 / NA	55	E281G (GAG→GGG)	hprS	sensory histidine kinase HprS
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (30/25); total (30/25)

ATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAG  >  NC_000913/2037180‑2037447

aTTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATg                                                                                                                                  <  2:759323/140‑1 (MQ=255)

 ttCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGt                                                                                                                                 >  2:6653/1‑140 (MQ=255)

 ttCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGt                                                                                                                                 >  2:823245/1‑140 (MQ=255)

 ttCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGt                                                                                                                                 >  2:1017020/1‑140 (MQ=255)

         aGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCa                                                                                                                         >  1:526765/1‑140 (MQ=255)

           tCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAAt                                                                                                                       >  2:964311/1‑140 (MQ=255)

                caaATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGttt                                                                                                                  >  2:903050/1‑140 (MQ=255)

                    ttttCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTg                                                                                                              <  1:964311/140‑1 (MQ=255)

                     tttCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGa                                                                                                             >  1:74989/1‑140 (MQ=255)

                           cTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCa                                                                                                       <  2:133452/140‑1 (MQ=255)

                            ttCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAg                                                                                                      <  2:386332/140‑1 (MQ=255)

                               cTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGa                                                                                                   <  2:24381/140‑1 (MQ=255)

                                        agaAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTgg                                                                                          >  2:694447/1‑140 (MQ=255)

                                        agaAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTgg                                                                                          >  2:69499/1‑140 (MQ=255)

                                        agaAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTgg                                                                                          >  1:259241/1‑140 (MQ=255)

                                        agaAAGCGAGTCCAGTTTCACCAAAACATTGTTTTCATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACt                                                                                               >  2:642727/1‑135 (MQ=255)

                                        agaAAGCGAGTCCAGTTTCACCAAAACATTGTTTTCATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACt                                                                                               <  1:642727/135‑1 (MQ=255)

                                                aGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTg                                                                                  >  2:643730/1‑140 (MQ=255)

                                                    cAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCg                                                                              <  2:701532/140‑1 (MQ=255)

                                                          cACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCt                                                                        <  1:6653/140‑1 (MQ=255)

                                                            ccAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAAcc                                                                         >  2:1042924/1‑137 (MQ=255)

                                                            ccAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAAcc                                                                         <  1:1042924/137‑1 (MQ=255)

                                                            ccAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAATATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGa                                                                      >  2:301413/1‑140 (MQ=255)

                                                               aaaCATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTc                                                                   <  2:666516/140‑1 (MQ=255)

                                                                aaCATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCt                                                                  >  2:799778/1‑140 (MQ=255)

                                                                  cATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGa                                                                <  1:643730/140‑1 (MQ=255)

                                                                    ttGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGAcc                                                              <  2:74989/140‑1 (MQ=255)

                                                                    ttGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGAcc                                                              <  1:879634/140‑1 (MQ=255)

                                                                    ttGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGAcc                                                              <  2:259241/140‑1 (MQ=255)

                                                                     tGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGAccc                                                             >  1:978017/1‑140 (MQ=255)

                                                                         tttATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTa                                                         <  1:239013/140‑1 (MQ=255)

                                                                         tttATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTa                                                         >  2:633597/1‑140 (MQ=255)

                                                                                 cccTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGAcc                                                              >  1:661101/1‑127 (MQ=255)

                                                                                 cccTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGAcc                                                              <  2:661101/127‑1 (MQ=255)

                                                                                 cccTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTa                                                 >  1:801537/1‑140 (MQ=255)

                                                                                 cccTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTa                                                 >  1:120850/1‑140 (MQ=255)

                                                                                      gCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTgg                                            <  1:903050/140‑1 (MQ=255)

                                                                                           aaaCAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTc                                       >  2:185733/1‑140 (MQ=255)

                                                                                               aGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAg                                   >  1:625365/1‑140 (MQ=255)

                                                                                                gTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTg                                             <  2:185294/129‑1 (MQ=255)

                                                                                                gTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTg                                             >  1:185294/1‑129 (MQ=255)

                                                                                                gTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGt                                  >  2:162136/1‑140 (MQ=255)

                                                                                                     ttCTCTGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATg                             <  1:40482/140‑1 (MQ=255)

                                                                                                       ctctGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTACTTGGCGTTCTAAGTTCATGAg                           <  2:801537/140‑1 (MQ=255)

                                                                                                       ctctGTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAg                           <  2:120850/140‑1 (MQ=255)

                                                                                                           gTTAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCgag                       >  1:1126036/1‑140 (MQ=255)

                                                                                                             tAACCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGAt                     >  1:1030817/1‑140 (MQ=255)

                                                                                                               aCCGCGAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCg                   >  1:294155/1‑140 (MQ=255)

                                                                                                                 cgcgAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCAt                              <  2:680944/127‑1 (MQ=255)

                                                                                                                 cgcgAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCAt                              >  1:680944/1‑127 (MQ=255)

                                                                                                                  gcgAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCa                <  1:1017020/140‑1 (MQ=255)

                                                                                                                   cgAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGa                            >  2:63511/1‑127 (MQ=255)

                                                                                                                   cgAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGa                            <  1:63511/127‑1 (MQ=255)

                                                                                                                   cgAAATATTTTCCAGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAg               >  1:283584/1‑140 (MQ=255)

                                                                                                                                aGCCCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAg  <  2:625365/140‑1 (MQ=255)

ATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGATACTTCTGGTTTGACTGAGCGTAACCTGATTCTGACCCAGTAATGCATTAATTGGCGTTCTAAGTTCATGAGCGAGATCGTCAGCAAACTGACTTAG  >  NC_000913/2037180‑2037447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A