Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,047,440	G→A	G835S (GGC→AGC)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,047,440	0	G	A	100.0%	140.4 / NA	40	G835S (GGC→AGC)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/17); total (23/17)

AATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTT  >  NC_000913/2047308‑2047575

aaTCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTc                                                                                                                                  <  2:723858/140‑1 (MQ=255)

  tCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGa                                                                                                                                >  2:873998/1‑140 (MQ=255)

           cGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTgcgc                                                                                                                       >  1:920973/1‑140 (MQ=255)

           cGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTgcgc                                                                                                                       >  2:15384/1‑140 (MQ=255)

            gCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCa                                                                                                                      >  2:665205/1‑140 (MQ=255)

            gCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCa                                                                                                                      >  1:608182/1‑140 (MQ=255)

              aaaaaCGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAATAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAgg                                                                                                                    <  2:428143/140‑1 (MQ=255)

                             tGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGt                                                                                                     >  2:195937/1‑140 (MQ=255)

                                  tGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTCTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGaaa                                                                                                >  2:743462/1‑140 (MQ=255)

                                   ggCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGaaaa                                                                                               <  2:164658/140‑1 (MQ=255)

                                    gCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGGCAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGaaaaa                                                                                              >  2:1002803/1‑140 (MQ=255)

                                    gCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGaaaaa                                                                                              >  1:260007/1‑140 (MQ=255)

                                                   aaaaGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGa                                                                               >  2:255971/1‑140 (MQ=255)

                                                      agtagtAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACgg                                                                            <  1:873998/140‑1 (MQ=255)

                                                           taAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATg                                                                       >  1:1129227/1‑140 (MQ=255)

                                                             aGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGAc                                                                     >  1:940250/1‑140 (MQ=255)

                                                                  gAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGAc                                                                              >  1:368009/1‑126 (MQ=255)

                                                                  gAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGAc                                                                              <  2:368009/126‑1 (MQ=255)

                                                                                         ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCggg                                         >  2:608224/1‑140 (MQ=255)

                                                                                         ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCggg                                         >  2:933363/1‑140 (MQ=255)

                                                                                         ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCggg                                         >  2:859771/1‑140 (MQ=255)

                                                                                         ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCggg                                         >  2:915065/1‑140 (MQ=255)

                                                                                         ccTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCggg                                         <  2:920973/140‑1 (MQ=255)

                                                                                             gAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGc                                     <  1:915065/140‑1 (MQ=255)

                                                                                                  tGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAg                                <  1:608224/140‑1 (MQ=255)

                                                                                                   ggCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAgg                               >  1:785335/1‑140 (MQ=255)

                                                                                                   ggCGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAgg                               >  1:244572/1‑140 (MQ=255)

                                                                                                     cGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCa                             >  2:516841/1‑140 (MQ=255)

                                                                                                     cGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCa                             <  2:940250/140‑1 (MQ=255)

                                                                                                     cGTGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCa                             <  1:291994/140‑1 (MQ=255)

                                                                                                       tGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAAc                           <  1:411622/140‑1 (MQ=255)

                                                                                                       tGAAACAAACGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAAc                           <  1:1069370/140‑1 (MQ=255)

                                                                                                           acaaacGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGc                       <  1:280932/140‑1 (MQ=255)

                                                                                                            caaacGTTAATCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCt                      <  1:516841/140‑1 (MQ=255)

                                                                                                                     aTCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAAc                           >  2:168696/1‑126 (MQ=255)

                                                                                                                     aTCGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAAc                           <  1:168696/126‑1 (MQ=255)

                                                                                                                       cGTCAGTTTTGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCa           <  2:260007/140‑1 (MQ=255)

                                                                                                                             ttttGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCa     <  1:859771/140‑1 (MQ=255)

                                                                                                                                tGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCTTGGTCACtt  >  1:1031603/1‑140 (MQ=255)

                                                                                                                                tGTCAGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACtt  >  1:578696/1‑140 (MQ=255)

AATCAAAACACCGCAAAAACGGATGTTAATGGTCTGGCGACTTTTGATCTGAAAAGTAGTAAGCAGGAAGACAACACGGTTGAAGTCACCCTTGAAAATGGCGTGAAACAAACGTTAATCGTCAGTTTTGTCGGCGACTCGAGTACTGCGCAGGTTGATCTGCAGAAGTCGAAAAATGAAGTGGTTGCTGACGGCAATGACAGCGTCACAATGACCGCGACCGTCCGGGATGCAAAAGGCAACCTGCTCAATGACGTCATGGTCACTT  >  NC_000913/2047308‑2047575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A