Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,096,693	T→C	V27A (GTA→GCA)	hisI →	putative bifunctional phosphoribosyl‑AMP cyclohydrolase/phosphoribosyl‑ATP pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,096,693	0	T	C	100.0%	293.0 / NA	81	V27A (GTA→GCA)	hisI	putative bifunctional phosphoribosyl‑AMP cyclohydrolase/phosphoribosyl‑ATP pyrophosphatase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (45/36); total (45/36)

AATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCC  >  NC_000913/2096557‑2096827

aatCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATc                                                                                                                                     <  1:997301/140‑1 (MQ=255)

   cAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCgg                                                                                                                                  <  1:963953/140‑1 (MQ=255)

     atatTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCg                                                                                                                                <  1:904539/140‑1 (MQ=255)

      tatTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGa                                                                                                                               >  1:494667/1‑140 (MQ=255)

           gTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGc                                                                                                                          <  2:494667/140‑1 (MQ=255)

                ttAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATg                                                                                                                     >  2:1019234/1‑140 (MQ=255)

                ttAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATg                                                                                                                     >  2:907219/1‑140 (MQ=255)

                     aGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGTAACACGCGGCATCCGGc                                                                                                                                 <  1:603877/123‑1 (MQ=255)

                     aGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGTAACACGCGGCATCCGGc                                                                                                                                 >  2:603877/1‑123 (MQ=255)

                     aGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTggg                                                                                                                <  2:705915/140‑1 (MQ=255)

                     aGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTggg                                                                                                                <  2:1005989/140‑1 (MQ=255)

                          aCCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCtata                                                                                                           >  2:467833/1‑140 (MQ=255)

                          aCCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCtata                                                                                                           >  1:1079093/1‑140 (MQ=255)

                          aCCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCtata                                                                                                           >  2:19400/1‑140 (MQ=255)

                                   cacaGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCgg                                                                                                  >  1:678491/1‑140 (MQ=255)

                                   cacaGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCgg                                                                                                  <  1:1019234/140‑1 (MQ=255)

                                      aGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAg                                                                                               >  2:553597/1‑140 (MQ=255)

                                        ggCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGTAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGcc                                                                                             <  1:1027844/140‑1 (MQ=255)

                                             ggAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGa                                                                                        >  1:518420/1‑140 (MQ=255)

                                               agaTCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACa                                                                                      <  2:678491/140‑1 (MQ=255)

                                                 aTCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACaaa                                                                                    >  2:1089514/1‑140 (MQ=255)

                                                  tCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACaaaa                                                                                   >  2:906183/1‑140 (MQ=255)

                                                    aGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAAcc                                                                                 >  2:244249/1‑140 (MQ=255)

                                                      gATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACaaa                                                                                    <  2:146652/135‑1 (MQ=255)

                                                      gATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACaaa                                                                                    >  1:146652/1‑135 (MQ=255)

                                                      gATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACaaa                                                                                    <  2:787255/135‑1 (MQ=255)

                                                      gATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACaaa                                                                                    >  1:787255/1‑135 (MQ=255)

                                                           gTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGaaa                                                                          >  1:525019/1‑140 (MQ=255)

                                                           gTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGaaa                                                                          >  2:310664/1‑140 (MQ=255)

                                                           gTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGaaa                                                                          >  1:951115/1‑140 (MQ=255)

                                                           gTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGaaa                                                                          >  2:880068/1‑140 (MQ=255)

                                                           gTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGaaa                                                                          >  1:868861/1‑140 (MQ=255)

                                                              aaCAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCg                                                                       <  1:680953/140‑1 (MQ=255)

                                                              aaCAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCg                                                                       >  2:947526/1‑140 (MQ=255)

                                                                cAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGc                                                                     >  2:1024278/1‑140 (MQ=255)

                                                                cAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGc                                                                     >  2:815865/1‑140 (MQ=255)

                                                                cAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGc                                                                     >  1:229796/1‑140 (MQ=255)

                                                                cAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGc                                                                     >  1:549972/1‑140 (MQ=255)

                                                                    acaacaacGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAg                                                                 >  1:1031685/1‑140 (MQ=255)

                                                                             tCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCa                                                                    <  1:371790/128‑1 (MQ=255)

                                                                             tCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCa                                                                    >  2:371790/1‑128 (MQ=255)

                                                                             tCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACcttct                                                        >  1:542209/1‑140 (MQ=255)

                                                                             tCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACcttct                                                        >  1:596011/1‑140 (MQ=255)

                                                                             tCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACcttct                                                        >  1:421871/1‑140 (MQ=255)

                                                                                cgAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTtcttct                                                     >  1:684491/1‑140 (MQ=255)

                                                                                   actggactggGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTcgc                                                  <  1:467833/140‑1 (MQ=255)

                                                                                   actggactggGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTcgc                                                  >  2:206684/1‑140 (MQ=255)

                                                                                    ctggactggGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTcgcg                                                 <  1:206684/140‑1 (MQ=255)

                                                                                      ggactggGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCa                                               <  1:119998/140‑1 (MQ=255)

                                                                                      ggactggGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCa                                               >  1:455650/1‑140 (MQ=255)

                                                                                            ggAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAAc                                         >  1:1080571/1‑140 (MQ=255)

                                                                                            ggAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAAc                                         >  1:412753/1‑140 (MQ=255)

                                                                                             gAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACa                                        >  2:486029/1‑140 (MQ=255)

                                                                                              aaaaaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAg                                       <  2:1080571/140‑1 (MQ=255)

                                                                                              aaaaaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAg                                       <  2:1031685/140‑1 (MQ=255)

                                                                                               aaaaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGc                                      <  1:1024278/140‑1 (MQ=255)

                                                                                               aaaaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGc                                      <  1:19400/140‑1 (MQ=255)

                                                                                               aaaaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGc                                      <  1:310664/140‑1 (MQ=255)

                                                                                                 aaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAg                                       >  2:534403/1‑137 (MQ=255)

                                                                                                 aaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAg                                       <  1:534403/137‑1 (MQ=255)

                                                                                                 aaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGa                                    <  1:1089514/140‑1 (MQ=255)

                                                                                                 aaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGa                                    <  2:542209/140‑1 (MQ=255)

                                                                                                 aaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGa                                    <  1:815865/140‑1 (MQ=255)

                                                                                                 aaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGa                                    <  2:596011/140‑1 (MQ=255)

                                                                                                 aaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGa                                    >  1:300193/1‑140 (MQ=255)

                                                                                                 aaaCCGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGa                                    >  1:446702/1‑140 (MQ=255)

                                                                                                    ccGACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACtg                                 <  1:244249/140‑1 (MQ=255)

                                                                                                      gACGGACTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACtgtg                               >  1:949501/1‑140 (MQ=255)

                                                                                                           aCTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCa                          <  2:951115/140‑1 (MQ=255)

                                                                                                           aCTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCa                          <  2:229796/140‑1 (MQ=255)

                                                                                                           aCTTATGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCa                          <  2:868861/140‑1 (MQ=255)

                                                                                                               aTGCCGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAgg                      >  2:688239/1‑140 (MQ=255)

                                                                                                                   cGGTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGaa                  <  2:949501/140‑1 (MQ=255)

                                                                                                                     gTGATTGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAAc                >  1:994120/1‑140 (MQ=255)

                                                                                                                         ttGTGCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCg            >  2:1111099/1‑140 (MQ=255)

                                                                                                                             gCAACACGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCa        <  1:906183/140‑1 (MQ=255)

                                                                                                                                 cacGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAAttt    <  2:455650/140‑1 (MQ=255)

                                                                                                                                 cacGCGGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAAttt    <  2:446702/140‑1 (MQ=255)

                                                                                                                                   cgcgGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCgg           >  2:690678/1‑131 (MQ=255)

                                                                                                                                   cgcgGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCgg           <  1:690678/131‑1 (MQ=255)

                                                                                                                                   cgcgGCATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTcc  >  1:126858/1‑140 (MQ=255)

AATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGCGAAGTGCTAATGCTGGGCTATATGAACCCGGAAGCCTTAGACAAAACCCTCGAAAGCGGCAAAGTCACCTTCTTCTCGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCC  >  NC_000913/2096557‑2096827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A