Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,142,531 T→C T140A (ACC→GCC)  udk ← uridine/cytidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,142,5310TC100.0% 204.6 / NA 57T140A (ACC→GCC) udkuridine/cytidine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/26);  total (31/26)

TGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCT  >  NC_000913/2142395‑2142663
                                                                                                                                         |                                                                                                                                    
tGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATc                                                                                                                                   >  1:1025290/1‑140 (MQ=255)
 gTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCa                                                                                                                                  >  2:573894/1‑140 (MQ=255)
          gCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATg                                                                                                                         >  1:528128/1‑140 (MQ=255)
          gCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATg                                                                                                                         >  2:408873/1‑140 (MQ=255)
          gCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTACGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATg                                                                                                                         >  2:391324/1‑140 (MQ=255)
            tCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGga                                                                                                                       >  1:866722/1‑140 (MQ=255)
             cAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGgag                                                                                                                      <  1:573894/140‑1 (MQ=255)
             cAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGgag                                                                                                                      <  2:433835/140‑1 (MQ=255)
                     tGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGaagttaag                                                                                                              >  2:891626/1‑140 (MQ=255)
                            aCATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCa                                                                                                       >  2:642863/1‑140 (MQ=255)
                                  ggCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCaaa                                                                                                 >  2:239797/1‑140 (MQ=255)
                                  ggCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCaaa                                                                                                 >  1:653734/1‑140 (MQ=255)
                                   gcgcACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAc                                                                                                <  1:1014602/140‑1 (MQ=255)
                                    cgcACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAcg                                                                                               >  2:86359/1‑140 (MQ=255)
                                     gcACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAcgc                                                                                              >  1:796440/1‑140 (MQ=255)
                                            tttttGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCg                                                                                       <  2:866722/140‑1 (MQ=255)
                                              tttGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGt                                                                                      <  2:561206/140‑1 (MQ=255)
                                              tttGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTc                                                                                     <  1:239797/140‑1 (MQ=255)
                                                     ttGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGcaaca                                                                              >  1:347024/1‑140 (MQ=255)
                                                     ttGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGcaaca                                                                              >  1:400459/1‑140 (MQ=255)
                                                     ttGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGcaaca                                                                              >  2:857136/1‑140 (MQ=255)
                                                     ttGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGcaaca                                                                              >  2:860380/1‑140 (MQ=255)
                                                     ttGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGcaaca                                                                              >  2:187800/1‑140 (MQ=255)
                                                         gcCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAAt                                                                          <  1:860380/140‑1 (MQ=255)
                                                               aCTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTc                                                                    <  1:86359/140‑1 (MQ=255)
                                                                   aaTCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCgaga                                                                >  2:248727/1‑140 (MQ=255)
                                                                       cATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAatga                                                            <  1:248727/140‑1 (MQ=255)
                                                                       cATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAatga                                                            <  1:891626/140‑1 (MQ=255)
                                                                        aTTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAatgat                                                           >  1:973000/1‑140 (MQ=255)
                                                                        aTTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAatgat                                                           >  2:118723/1‑140 (MQ=255)
                                                                         ttGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAatgatg                                                          >  1:238490/1‑140 (MQ=255)
                                                                             aCGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACct                                                      >  2:965181/1‑140 (MQ=255)
                                                                              cGCCCACGCTCGTTAACTTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACctt                                                     >  2:1053901/1‑140 (MQ=255)
                                                                                      cTCGTTAACGTCACGCTTGATGCGGCGCGTCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCt                                             >  1:275319/1‑140 (MQ=255)
                                                                                          ttAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAAc                                         >  1:1121973/1‑140 (MQ=255)
                                                                                               gTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCa                                    <  2:347024/140‑1 (MQ=255)
                                                                                                   cGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGt                                >  1:316956/1‑140 (MQ=255)
                                                                                                   cGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGt                                >  1:362089/1‑140 (MQ=255)
                                                                                                    gCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGtt                               <  2:653734/140‑1 (MQ=255)
                                                                                                           gcggcgCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCa                        <  2:238490/140‑1 (MQ=255)
                                                                                                             ggcgCATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATa                      <  1:962415/140‑1 (MQ=255)
                                                                                                                gcATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcgc                   <  2:973000/140‑1 (MQ=255)
                                                                                                                gcATCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcgc                   <  1:965181/140‑1 (MQ=255)
                                                                                                                  aTCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGt                 <  1:857136/140‑1 (MQ=255)
                                                                                                                  aTCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGt                 <  2:1025290/140‑1 (MQ=255)
                                                                                                                  aTCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGt                 >  1:628604/1‑140 (MQ=255)
                                                                                                                  aTCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGt                 <  1:187800/140‑1 (MQ=255)
                                                                                                                  aTCAGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGt                 >  1:63967/1‑140 (MQ=255)
                                                                                                                     aGGCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAGATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATg              <  2:796440/140‑1 (MQ=255)
                                                                                                                       gCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGtt            <  2:63967/140‑1 (MQ=255)
                                                                                                                       gCAGATATCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGtt            <  1:1053901/140‑1 (MQ=255)
                                                                                                                            tatCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACa       <  2:400459/140‑1 (MQ=255)
                                                                                                                             atCCAGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACAt      <  2:628604/140‑1 (MQ=255)
                                                                                                                                 aGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACAt      >  2:726654/1‑136 (MQ=255)
                                                                                                                                 aGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACAt      <  1:726654/136‑1 (MQ=255)
                                                                                                                                 aGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCt  <  1:408873/140‑1 (MQ=255)
                                                                                                                                 aGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCt  >  1:185514/1‑140 (MQ=255)
                                                                                                                                 aGC‑GGGGCATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCt  >  2:108402/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                    
TGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGC‑GGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTTCAACATAGCT  >  NC_000913/2142395‑2142663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A