Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,214,839	C→T	intergenic (‑195/‑27)	btsS ← / → mlrA	high‑affinity pyruvate receptor/DNA‑binding transcriptional activator MlrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,214,839	0	C	T	100.0%	113.9 / NA	33	intergenic (‑195/‑27)	btsS/mlrA	high‑affinity pyruvate receptor/DNA‑binding transcriptional activator MlrA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/19); total (14/19)

TCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACCGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCAGAGGCGTTACGGATTGCTGAAACCGCAACGGACAGACGG  >  NC_000913/2214702‑2214975

tctttGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTgg                                                                                                                                        >  2:180786/1‑140 (MQ=255)

  tttGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGa                                                                                                                                      <  2:528809/140‑1 (MQ=255)

             gCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCa                                                                                                                           <  1:439209/140‑1 (MQ=255)

             gCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCa                                                                                                                           <  2:348693/140‑1 (MQ=255)

              cAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCaa                                                                                                                          <  1:180786/140‑1 (MQ=255)

                           aTTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATg                                                                                                             <  1:377521/140‑1 (MQ=255)

                              cGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGgcg                                                                                                          <  1:903075/140‑1 (MQ=255)

                                 gAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCtt                                                                                                       <  2:30889/140‑1 (MQ=255)

                                       gATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTAcaca                                                                                                 <  1:771946/140‑1 (MQ=255)

                                           aCTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTg                                                                                             <  1:815975/140‑1 (MQ=255)

                                            cTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTgg                                                                                            >  1:327701/1‑140 (MQ=255)

                                             tCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGt                                                                                           <  1:265532/140‑1 (MQ=255)

                                              cAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCt                                                                                                        >  2:1030180/1‑126 (MQ=255)

                                              cAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCt                                                                                                        <  1:1030180/126‑1 (MQ=255)

                                              cAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTg                                                                                          >  2:924686/1‑140 (MQ=255)

                                              cAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTg                                                                                          >  1:992823/1‑140 (MQ=255)

                                               aaaaTCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGa                                                                                         >  2:974594/1‑140 (MQ=255)

                                                 aaTCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAg                                                                                       >  2:852120/1‑140 (MQ=255)

                                                               aataaaatCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTtgt                                                                         <  1:852120/140‑1 (MQ=255)

                                                                           cTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCt                                                             <  1:974594/140‑1 (MQ=255)

                                                                                    cacaAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTa                                                    >  2:871667/1‑140 (MQ=255)

                                                                                     acaAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTAc                                                   <  1:339815/140‑1 (MQ=255)

                                                                                      caAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTAcg                                                  >  1:519425/1‑140 (MQ=255)

                                                                                      caAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTAcg                                                  >  1:115292/1‑140 (MQ=255)

                                                                                                  aaaaCTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCagag                                      <  2:115292/140‑1 (MQ=255)

                                                                                                             gTTCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCAGAGGCGTTACGGAt                           <  2:327701/140‑1 (MQ=255)

                                                                                                              ttCGCAAAACTGCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCAGAGGCGTTACGGAtt                          >  1:356992/1‑140 (MQ=255)

                                                                                                                         gCGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCAGAGGCGTTACGGATTGCTGAAACCGc               <  2:519425/140‑1 (MQ=255)

                                                                                                                          cGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCAGAGGCGTTACgg                             >  2:1105541/1‑125 (MQ=255)

                                                                                                                          cGTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCAGAGGCGTTACgg                             <  1:1105541/125‑1 (MQ=255)

                                                                                                                           gTCTAAAGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCAGAGGCGTTACGGATTGCTGAAACCGCaa             >  1:395815/1‑140 (MQ=255)

                                                                                                                               aaaGTTAAACTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCAGAGGCGTTACGGATTGCTGAAACCGCAACGGa         >  1:346583/1‑140 (MQ=255)

                                                                                                                                      aaCTGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCAGAGGCGTTACGGATTGCTGAAACCGCAACGGACAGAcgg  <  2:346583/140‑1 (MQ=255)

TCTTTGAACTCTTGCAGAAAAATGAGAATTCGTGAGTACGATCACTCAAAATCGCCTGGCAAAAATAAAATCACCCTATAGATGCACAAAAAACGGGCAAAACTACCTGGTTCGCAAAACTGCGTCTAAAGTTAAACCGGGACCTCGCGAGCAAGGGTGAGACGATGGCGCTTTACACAATTGGTGAAGTGGCGTTGCTTTGTGATATTAATCCTGTCACGTTACGCGCGTGGCAGAGGCGTTACGGATTGCTGAAACCGCAACGGACAGACGG  >  NC_000913/2214702‑2214975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A