Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F28 I1 R1
|
180 |
66.5 |
2263572 |
99.4% |
2249990 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,218,611 |
(T)6→7 |
coding (871/918 nt) |
osmF ← |
glycine betaine ABC transporter periplasmic binding protein OsmF |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,218,605 | 1 | . | T | 97.5%
| 137.0
/ ‑3.8
| 40 | coding (877/918 nt) | osmF | glycine betaine ABC transporter periplasmic binding protein OsmF |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (21/18); total (21/19) |
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CAC‑TTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCA > NC_000913/2218479‑2218739
|
tGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATc < 2:51067/140‑1 (MQ=255)
gATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATcc > 2:918209/1‑140 (MQ=255)
cGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCt > 1:584543/1‑140 (MQ=255)
cAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTcc > 1:633626/1‑140 (MQ=255)
gcgcCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGt > 2:720717/1‑132 (MQ=255)
gcgcCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGt < 1:720717/132‑1 (MQ=255)
tGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATg < 2:122426/140‑1 (MQ=255)
cGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTATTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCt > 1:743076/1‑140 (MQ=255)
cAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGt < 2:633626/140‑1 (MQ=255)
cAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGAAGTCGGCAGC‑CACTTTTTTTGGCATCCAGACCTTCCACAGCAATGCTGGCATTCAGTtgc > 1:190155/1‑140 (MQ=255)
cagcagcagCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTtgctgc > 2:920160/1‑140 (MQ=255)
agcagcagcGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTCCTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCa < 1:420515/140‑1 (MQ=255)
ccAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGc > 1:1091473/1‑140 (MQ=255)
cAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCa > 1:760051/1‑140 (MQ=255)
ggATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGc > 2:1108781/1‑140 (MQ=255)
aaTACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCg < 1:348632/140‑1 (MQ=255)
aTACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGa > 2:375077/1‑140 (MQ=255)
aTACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGa > 1:217079/1‑140 (MQ=255)
gAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCg > 1:713473/1‑134 (MQ=255)
gAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCg < 2:713473/134‑1 (MQ=255)
gAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACt > 2:245297/1‑140 (MQ=255)
gAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCAGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACt > 2:709809/1‑140 (MQ=255)
gAAATAAGTCACGGGAAATTACTTCGTCCACCCTTATTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACt < 2:743076/140‑1 (MQ=255)
aaaTAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTg < 1:887552/140‑1 (MQ=255)
aaaTAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTg > 2:880657/1‑140 (MQ=255)
aaTAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTgg < 2:584543/140‑1 (MQ=255)
cACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGAt < 1:245297/140‑1 (MQ=255)
cACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGAt < 2:780960/140‑1 (MQ=255)
cccTTTTTGTTTCAGGTAGTCGGCAGCGCCT‑TTTTTTGGCATCCAGTCCTTCCACAGAAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATAc < 2:190155/140‑1 (MQ=255)
ttttGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTccc < 2:760051/140‑1 (MQ=255)
tttGTTTCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCt > 2:406265/1‑140 (MQ=255)
tCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTc > 2:867740/1‑135 (MQ=255)
tCAGGTAGTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTc < 1:867740/135‑1 (MQ=255)
gTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCa > 1:75181/1‑140 (MQ=255)
gTCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCa < 1:375077/140‑1 (MQ=255)
tCGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCAc > 1:214529/1‑140 (MQ=255)
cGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCATAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACg < 1:918209/140‑1 (MQ=255)
cGGCAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACg < 2:214529/140‑1 (MQ=255)
cAGC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcac < 1:709809/140‑1 (MQ=255)
gC‑CACTTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcacca > 2:213107/1‑140 (MQ=255)
|
TGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGC‑CAC‑TTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCA > NC_000913/2218479‑2218739
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
