Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,306,954	C→T	intergenic (‑200/+18)	mqo ← / ← yojI	malate:quinone oxidoreductase/ABC transporter family protein/microcin J25 efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,306,954	0	C	T	100.0%	194.6 / NA	55	intergenic (‑200/+18)	mqo/yojI	malate:quinone oxidoreductase/ABC transporter family protein/microcin J25 efflux protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/26); total (29/26)

CAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGATAAAGTAATGA  >  NC_000913/2306829‑2307086

caacaTTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTa                                                                                                                        <  2:35941/140‑1 (MQ=255)

caacaTTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTa                                                                                                                        >  1:175627/1‑140 (MQ=255)

  acaTTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAat                                                                                                                      >  1:925858/1‑140 (MQ=255)

              tGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTcc                                                                                                          >  1:603153/1‑140 (MQ=255)

                   ataAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCa                                                                                                     >  1:512429/1‑140 (MQ=255)

                        ttATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGc                                                                                                >  1:672812/1‑140 (MQ=255)

                                 aaTTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGa                                                                                       <  2:603153/140‑1 (MQ=255)

                                  aTTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGaa                                                                                      >  1:534164/1‑140 (MQ=255)

                                     ttAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCg                                                                                   <  2:925858/140‑1 (MQ=255)

                                             aTTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATcgcg                                                                           >  1:685651/1‑140 (MQ=255)

                                                       cGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGccc                                                                 >  2:1045253/1‑140 (MQ=255)

                                                       cGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGccc                                                                 >  1:950674/1‑140 (MQ=255)

                                                        gTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCg                                                                >  1:822334/1‑140 (MQ=255)

                                                         ttGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGt                                                               >  2:647221/1‑140 (MQ=255)

                                                           gATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCa                                                             <  2:950674/140‑1 (MQ=255)

                                                                      gggTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTGTTCGCCCGTCAGCTCGCTAAGt                                                  >  1:799695/1‑140 (MQ=255)

                                                                       ggTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTc                                                              <  1:757142/127‑1 (MQ=255)

                                                                       ggTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTc                                                              >  2:757142/1‑127 (MQ=255)

                                                                       ggTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGtt                                                 >  1:252818/1‑140 (MQ=255)

                                                                         ttattaGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGc                                               <  2:685651/140‑1 (MQ=255)

                                                                           attaGAAATGAAGGGGTTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGccc                                             >  1:1033192/1‑140 (MQ=255)

                                                                           attaGAAATGAAGGGATTTTTAGCATATTGACCTGACGTCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGccc                                             >  2:951063/1‑140 (MQ=255)

                                                                           attaGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGccc                                             >  1:456960/1‑140 (MQ=255)

                                                                           attaGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGccc                                             >  2:929003/1‑140 (MQ=255)

                                                                           attaGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGccc                                             >  1:1057272/1‑140 (MQ=255)

                                                                           attaGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCCTCGCCCGTCAGCTCGCTAAGTTGccc                                             >  1:168631/1‑140 (MQ=255)

                                                                           attaGAAATGAAGGGATCTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTg                                                >  1:245459/1‑137 (MQ=255)

                                                                           attaGAAATGAAGGGATCTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTg                                                <  2:245459/137‑1 (MQ=255)

                                                                             taGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCAt                                           <  1:840222/140‑1 (MQ=255)

                                                                                 aaTGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTgcg                                       <  1:1045253/140‑1 (MQ=255)

                                                                                 aaTGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTgcg                                       >  1:458554/1‑140 (MQ=255)

                                                                                  aTGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTgcgc                                      >  1:522234/1‑140 (MQ=255)

                                                                                   tGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGc                                               >  2:77155/1‑130 (MQ=255)

                                                                                   tGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGc                                               <  1:77155/130‑1 (MQ=255)

                                                                                    gAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCAt                                    <  2:1057272/140‑1 (MQ=255)

                                                                                       gggATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTc                                 >  2:939537/1‑140 (MQ=255)

                                                                                          aTTTTTAGCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCcag                              >  2:248255/1‑140 (MQ=255)

                                                                                                 gCATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCgg                       <  1:248255/140‑1 (MQ=255)

                                                                                                  cATATTGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGt                      <  1:731172/140‑1 (MQ=255)

                                                                                                      ttGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGc                  <  2:456960/140‑1 (MQ=255)

                                                                                                       tGACCTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCg                 <  2:512429/140‑1 (MQ=255)

                                                                                                           cTGACGTCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGa             <  1:951063/140‑1 (MQ=255)

                                                                                                           cTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGa             <  2:1033192/140‑1 (MQ=255)

                                                                                                           cTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGa             <  2:215326/140‑1 (MQ=255)

                                                                                                           cTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGa             <  2:151494/140‑1 (MQ=255)

                                                                                                           cTGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCCTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGa             <  2:168631/140‑1 (MQ=255)

                                                                                                            tGACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGAt            <  1:998556/140‑1 (MQ=255)

                                                                                                             gACGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGATa           <  1:929003/140‑1 (MQ=255)

                                                                                                              aCGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGATaa          >  2:672550/1‑140 (MQ=255)

                                                                                                              aCGGCAGCAGTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGATaa          >  2:1120787/1‑140 (MQ=255)

                                                                                                                   agcagTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCgg                   <  1:94554/126‑1 (MQ=255)

                                                                                                                   agcagTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCgg                   >  2:94554/1‑126 (MQ=255)

                                                                                                                     cagTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGATAAAGTAATg   <  2:799695/140‑1 (MQ=255)

                                                                                                                     cagTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGATAAAGTAATg   <  2:175627/140‑1 (MQ=255)

                                                                                                                      agTCCGCTAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGATAAAGTAATGa  <  2:458554/140‑1 (MQ=255)

CAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAGCAGTCCGCCAGGTCAGCGTCGTAATATTATGCCGTCCGGGCAACGGCATCACGCGAAGCGGCATCGCGCTCTTCGCCCGTCAGCTCGCTAAGTTGCCCATTGCGCATTTCCAGCAGGCGGTCGGCGTGGATAAAGTAATGA  >  NC_000913/2306829‑2307086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A