Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 180 66.5 2263572 99.4% 2249990 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,529,863 T→C N105S (AAC→AGC)  ligA ← DNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,529,8630TC100.0% 280.2 / NA 78N105S (AAC→AGC) ligADNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/36);  total (42/36)

CACATTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGTTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCT  >  NC_000913/2529730‑2529991
                                                                                                                                     |                                                                                                                                
cacaTTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGtttt                                                                                                                            <  2:267757/140‑1 (MQ=255)
   aTTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCa                                                                                                                         >  1:229751/1‑140 (MQ=255)
    ttAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAg                                                                                                                        >  2:933072/1‑140 (MQ=255)
    ttAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAg                                                                                                                        >  1:126176/1‑140 (MQ=255)
    ttAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAg                                                                                                                        >  2:882200/1‑140 (MQ=255)
     tAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCa                                                                                                                         <  1:687315/138‑1 (MQ=255)
     tAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCa                                                                                                                         >  2:687315/1‑138 (MQ=255)
     tAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGa                                                                                                                       <  2:991924/140‑1 (MQ=255)
     tAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGa                                                                                                                       <  1:612443/140‑1 (MQ=255)
     tAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGa                                                                                                                       >  1:941191/1‑140 (MQ=255)
     tAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGa                                                                                                                       <  2:623300/140‑1 (MQ=255)
             atatCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTg                                                                                                               >  2:53314/1‑140 (MQ=255)
             atatCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTg                                                                                                               >  2:922636/1‑140 (MQ=255)
             atatCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTg                                                                                                               >  1:928297/1‑140 (MQ=255)
             atatCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTg                                                                                                               >  1:718598/1‑140 (MQ=255)
               atCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGca                                                                                                             <  2:928297/140‑1 (MQ=255)
               atCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGca                                                                                                             <  1:435637/140‑1 (MQ=255)
                   tCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACg                                                                                                         >  1:614654/1‑140 (MQ=255)
                     cccGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGcaca                                                                                                           >  1:963659/1‑136 (MQ=255)
                     cccGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGcaca                                                                                                           <  2:963659/136‑1 (MQ=255)
                       cGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTttg                                                                                                     >  1:311307/1‑140 (MQ=255)
                         gtggtgCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTttgtt                                                                                                   <  1:922636/140‑1 (MQ=255)
                         gtggtgCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACCTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTttgtt                                                                                                   <  2:1039295/140‑1 (MQ=255)
                          tggtgCCATCGCCACAGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTttgttg                                                                                                  >  2:822992/1‑140 (MQ=255)
                            gtgCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGaa                                                                                                <  2:311307/140‑1 (MQ=255)
                                            tCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTttct                                                                                >  1:426353/1‑140 (MQ=255)
                                              gcgGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTttcttc                                                                              >  1:509876/1‑140 (MQ=255)
                                                 gCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTtcatc                                                                           >  1:182455/1‑140 (MQ=255)
                                                   aCTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCaa                                                                         <  2:126176/140‑1 (MQ=255)
                                                   aCTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCaa                                                                         <  1:933072/140‑1 (MQ=255)
                                                   aCTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCaa                                                                         <  1:53314/140‑1 (MQ=255)
                                                     tGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCaaaa                                                                       >  1:854982/1‑140 (MQ=255)
                                                       aCTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTttcttc                                                                              >  2:576220/1‑131 (MQ=255)
                                                       aCTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTttcttc                                                                              <  1:576220/131‑1 (MQ=255)
                                                             aCGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATc                                                               <  2:426353/140‑1 (MQ=255)
                                                                 cATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGt                                                           >  1:1031586/1‑140 (MQ=255)
                                                                 cATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGt                                                           >  2:761780/1‑140 (MQ=255)
                                                                    tttCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGAc                                                        <  1:1098920/140‑1 (MQ=255)
                                                                    tttCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGAc                                                        <  2:616964/140‑1 (MQ=255)
                                                                     ttCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACa                                                       <  1:822992/140‑1 (MQ=255)
                                                                        aTACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGAc                                                        >  2:337641/1‑136 (MQ=255)
                                                                        aTACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGAc                                                        <  1:337641/136‑1 (MQ=255)
                                                                         tACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCAt                                                   <  2:229751/140‑1 (MQ=255)
                                                                               aTACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTAc                                             >  2:1023437/1‑140 (MQ=255)
                                                                               aTACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTAc                                             <  1:1014474/140‑1 (MQ=255)
                                                                                 aCTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACtt                                           <  2:509876/140‑1 (MQ=255)
                                                                                   tGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCa                                         >  2:768274/1‑140 (MQ=255)
                                                                                     aCGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATg                                       >  2:13086/1‑140 (MQ=255)
                                                                                     aCGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATg                                       >  1:326536/1‑140 (MQ=255)
                                                                                     aCGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATg                                       >  2:388225/1‑140 (MQ=255)
                                                                                      cGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATgg                                      >  2:472055/1‑140 (MQ=255)
                                                                                      cGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATgg                                      >  2:781471/1‑140 (MQ=255)
                                                                                        gCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCg                                    >  1:912148/1‑140 (MQ=255)
                                                                                        gCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCg                                    >  2:228483/1‑140 (MQ=255)
                                                                                        gCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCg                                    >  2:464222/1‑140 (MQ=255)
                                                                                        gCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCg                                    >  1:259252/1‑140 (MQ=255)
                                                                                        gCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCg                                    >  1:184104/1‑140 (MQ=255)
                                                                                        gCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCg                                    >  2:815223/1‑140 (MQ=255)
                                                                                        gCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCg                                    >  1:30226/1‑140 (MQ=255)
                                                                                           aGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTAt                                 <  1:228483/140‑1 (MQ=255)
                                                                                                            cTCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCa                >  2:592297/1‑140 (MQ=255)
                                                                                                             tCACAGCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAg               <  2:1031586/140‑1 (MQ=255)
                                                                                                                  gCACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAgcggcg          <  1:1023437/140‑1 (MQ=255)
                                                                                                                   cACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGgcgc         <  2:259252/140‑1 (MQ=255)
                                                                                                                   cACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGgcgc         >  2:335301/1‑140 (MQ=255)
                                                                                                                   cACCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGgcgc         <  1:1117888/140‑1 (MQ=255)
                                                                                                                    aCCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCa        <  2:184104/140‑1 (MQ=255)
                                                                                                                    aCCAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCa        <  2:614654/140‑1 (MQ=255)
                                                                                                                     ccAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAg       <  2:30226/140‑1 (MQ=255)
                                                                                                                     ccAGGTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAg       <  2:941191/140‑1 (MQ=255)
                                                                                                                        ggTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGgcgc         >  2:730042/1‑135 (MQ=255)
                                                                                                                        ggTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGgcgc         <  1:730042/135‑1 (MQ=255)
                                                                                                                        ggTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCa        >  2:1020215/1‑136 (MQ=255)
                                                                                                                        ggTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCa        <  1:1020215/136‑1 (MQ=255)
                                                                                                                        ggTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAgcgc    >  1:280862/1‑140 (MQ=255)
                                                                                                                         gTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGAGAGCATTGGTACTTCATGGCGTATCTGGCTGAAACCCGCCAGCGGCGCAGCGcc   <  2:718598/140‑1 (MQ=255)
                                                                                                                         gTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGcc   >  2:68468/1‑140 (MQ=255)
                                                                                                                         gTGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGcc   <  1:464222/140‑1 (MQ=255)
                                                                                                                          tGACTTTCTCGCTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCt  <  2:326536/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                
CACATTAGACGTGATATCTTCCCCGGTGGTGCCATCGCCACGGGTCGCGGCACTGACTAAAACGCCATTTTCATACAGAATACTGACGGCAAGACCATCCAGCTTCAGCTCACAGCACCAGGTGACTTTCTCGTTGTTTTTCAGACGGTCCTGCACACGTTTGTTGAAAGCAAGAAAGCTTTCTTCATCAAAAACGTTATCCAGTGACAGCATTGGTACTTCATGGCGTATCTGGCTGAAAGCCGCCAGCGGCGCAGCGCCT  >  NC_000913/2529730‑2529991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A