Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,531,591	G→A	M43I (ATG→ATA)	cysZ →	sulfate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,531,591	0	G	A	100.0%	157.2 / NA	45	M43I (ATG→ATA)	cysZ	sulfate:H(+) symporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/23); total (22/23)

TCCTGATTATGGTTTCATCATTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCTGTTGTGGCCTCTGGCTGTCATCTCTGTGCTGTTAGTG  >  NC_000913/2531455‑2531723

tCCTGATTATGGTTTCATCATTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATaggg                                                                                                                                   >  1:347411/1‑140 (MQ=255)

tCCTGATTATGGTTTCATCATTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATaggg                                                                                                                                   >  2:707585/1‑140 (MQ=255)

       tATGGTTTCATCATTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCAt                                                                                                                            <  2:97711/140‑1 (MQ=255)

        aTGGTTTCATCATTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCAtt                                                                                                                           <  1:707585/140‑1 (MQ=255)

         tGGTTTCATCATTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTc                                                                                                                          >  2:677135/1‑140 (MQ=255)

          ggTTTCATCATTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCt                                                                                                                         <  1:497453/140‑1 (MQ=255)

              tcatcaTTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCtggtg                                                                                                                     <  1:245208/140‑1 (MQ=255)

              tcatcaTTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCtggtg                                                                                                                     <  1:583301/140‑1 (MQ=255)

              tcatcaTTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCtggtg                                                                                                                     <  2:138800/140‑1 (MQ=255)

              tcatcaTTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCtggtg                                                                                                                     <  1:629613/140‑1 (MQ=255)

              tcatcaTTCACATCTGCCCCACGCAGCAGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCtggtg                                                                                                                     <  1:615354/140‑1 (MQ=255)

               catcatTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCtggtg                                                                                                                     >  2:733930/1‑139 (MQ=255)

               catcatTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCtggtg                                                                                                                     <  1:733930/139‑1 (MQ=255)

                      cacaTCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTa                                                                                                             <  2:693389/140‑1 (MQ=255)

                      cacaTCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTa                                                                                                             >  2:568852/1‑140 (MQ=255)

                              ccccACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCtggtg                                                                                                                     <  1:564113/124‑1 (MQ=255)

                              ccccACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCtggtg                                                                                                                     >  2:564113/1‑124 (MQ=255)

                              ccccACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTc                                                                                                     >  1:195370/1‑140 (MQ=255)

                              ccccACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTc                                                                                                     >  1:441940/1‑140 (MQ=255)

                               cccACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCg                                                                                                    <  1:677135/140‑1 (MQ=255)

                                   cGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGt                                                                                                >  2:74735/1‑140 (MQ=255)

                                        cGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGa                                                                                           >  2:538233/1‑140 (MQ=255)

                                             tttACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCg                                                                                      >  1:526538/1‑140 (MQ=255)

                                              ttACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGa                                                                                     <  1:74735/140‑1 (MQ=255)

                                                       tGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGa                                                                            >  2:719219/1‑140 (MQ=255)

                                                         cgcAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGAct                                                                                   >  1:630251/1‑131 (MQ=255)

                                                         cgcAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGAct                                                                                   <  2:630251/131‑1 (MQ=255)

                                                         cgcAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGt                                                                          >  1:83537/1‑140 (MQ=255)

                                                         cgcAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGt                                                                          >  1:843522/1‑140 (MQ=255)

                                                         cgcAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGt                                                                          >  1:588048/1‑140 (MQ=255)

                                                          gcAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGtt                                                                         <  1:568852/140‑1 (MQ=255)

                                                               gCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATCGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGtt                                                                    <  2:426100/140‑1 (MQ=255)

                                                                    aaGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGa                                                               <  2:441940/140‑1 (MQ=255)

                                                                    aaGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGa                                                               <  2:83537/140‑1 (MQ=255)

                                                                    aaGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGa                                                               <  1:59008/140‑1 (MQ=255)

                                                                    aaGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGa                                                               <  1:406708/140‑1 (MQ=255)

                                                                          gTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACACCTCGATGCGTGGATCCCGACTCTCATGGGTTACCTTCCGGACTGGca                                                         >  2:684764/1‑139 (MQ=255)

                                                                                      gggATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAg                                             >  1:28616/1‑140 (MQ=255)

                                                                                             ggCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTAt                                         <  1:146173/137‑1 (MQ=255)

                                                                                             ggCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTAt                                         >  2:146173/1‑137 (MQ=255)

                                                                                                 tttCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCtgttgt                                  >  1:340958/1‑140 (MQ=255)

                                                                                                       tATTTTACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCTGTTGTGGCctc                            <  2:195370/140‑1 (MQ=255)

                                                                                                         ttttACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCTGTTGTGGCCTCTg                          <  2:1495/140‑1 (MQ=255)

                                                                                                         ttttACCGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCAATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCTGTTGTGGCCTCTg                          <  2:28616/140‑1 (MQ=255)

                                                                                                              ccGCTGCTGGTCAATATTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCTGTTGTGGCCTCTGGCTGt                     >  2:837773/1‑140 (MQ=255)

                                                                                                                            tatTTTGTTGATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCTGTTGTGGCCTCTGGCTGTCATCTCTGTGCTGt       >  2:677413/1‑140 (MQ=255)

                                                                                                                                 tgttgATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATACCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCTGTTGTGGCCTCTGGCTGTCATCTCTGTGCTGTTAgtg  >  2:724750/1‑140 (MQ=255)

                                                                                                                                    tgATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCAGACTGGCTACAATGGCTGAGTTATCTGTTGTGGCCTCTGGCTGTCATCTCtg             >  1:86524/1‑126 (MQ=255)

                                                                                                                                    tgATAGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCAGACTGGCTACAATGGCTGAGTTATCTGTTGTGGCCTCTGGCTGTCATCTCtg             <  2:86524/126‑1 (MQ=255)

TCCTGATTATGGTTTCATCATTCACATCTGCCCCACGCAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATCTGTTGTGGCCTCTGGCTGTCATCTCTGTGCTGTTAGTG  >  NC_000913/2531455‑2531723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A