Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,017	(C)_7→8	coding (200/2019 nt)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,010	1	.	C	97.4%	271.5 / ‑0.3	78	coding (193/2019 nt)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (34/42); total (35/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01

TCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAA  >  NC_000913/2601871‑2602143

tCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTg                                                                                                                                         >  1:1098373/1‑140 (MQ=255)

tCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTg                                                                                                                                         >  1:787348/1‑140 (MQ=255)

  tGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑cc                                                                                                                                     >  1:370921/1‑140 (MQ=255)

  tGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑cc                                                                                                                                     >  2:594427/1‑140 (MQ=255)

    gCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑cccc                                                                                                                                   <  2:370921/140‑1 (MQ=255)

     cTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑ccccc                                                                                                                                  <  1:488749/140‑1 (MQ=255)

     cTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑ccccc                                                                                                                                  <  2:863824/140‑1 (MQ=255)

     cTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑ccccc                                                                                                                                  <  1:594427/140‑1 (MQ=255)

     cTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑ccccc                                                                                                                                  <  1:798113/140‑1 (MQ=255)

      ttCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑cccccc                                                                                                                                 <  2:969374/140‑1 (MQ=255)

         gCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑cccccccct                                                                                                                              >  2:933548/1‑138 (MQ=255)

                   cTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTg                                                                                                                     <  1:278635/140‑1 (MQ=255)

                    tCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGc                                                                                                                    <  2:629238/140‑1 (MQ=255)

                    tCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGc                                                                                                                    <  2:1098373/140‑1 (MQ=255)

                       gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGa                                                                                                                 >  2:522825/1‑140 (MQ=255)

                       gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGa                                                                                                                 >  2:887983/1‑140 (MQ=255)

                       gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑ccccc                                                                                                                                  <  2:1008932/122‑1 (MQ=255)

                       gTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑ccccc                                                                                                                                  >  1:1008932/1‑122 (MQ=255)

                        tCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGAt                                                                                                                >  1:979771/1‑140 (MQ=255)

                           ggACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTa                                                                                                             <  2:682259/140‑1 (MQ=255)

                            gACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTaa                                                                                                            >  1:45161/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCt                                                                                                        >  1:800995/1‑139 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                       >  2:57583/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                       >  2:608508/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTg                                                                                                       >  1:758566/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTCTGAATTTGCCGATTTAACCCt                                                                                                        <  2:800995/139‑1 (MQ=255)

                                   tGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     <  2:385556/140‑1 (MQ=255)

                                   tGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     <  1:723433/140‑1 (MQ=255)

                                   tGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCg                                                                                                     <  2:758566/140‑1 (MQ=255)

                                    ggCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGa                                                                                                    <  1:106468/140‑1 (MQ=255)

                                      cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                                   >  1:242219/1‑139 (MQ=255)

                                      cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                                   <  2:242219/139‑1 (MQ=255)

                                      cTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAAt                                                                                                  >  2:394088/1‑140 (MQ=255)

                                         ttAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGa                                                                                               <  2:640557/140‑1 (MQ=255)

                                           aaGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATa                                                                                             >  2:720854/1‑140 (MQ=255)

                                           aaGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATa                                                                                             >  2:683497/1‑140 (MQ=255)

                                           aaGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATa                                                                                             >  1:162163/1‑140 (MQ=255)

                                            aGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAg                                                                                            <  2:527379/140‑1 (MQ=255)

                                                ttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACACTGCGAATGGATAGCctc                                                                                        >  1:212548/1‑140 (MQ=255)

                                                 tCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                                   >  1:236159/1‑128 (MQ=255)

                                                 tCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGaa                                                                                                   <  2:236159/128‑1 (MQ=255)

                                                 tCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                                       <  1:683497/140‑1 (MQ=255)

                                                  ccGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                                      <  1:608508/140‑1 (MQ=255)

                                                      cATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGCCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGc                                                                                   <  2:267771/140‑1 (MQ=255)

                                                      cATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                                      <  2:718838/136‑1 (MQ=255)

                                                      cATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCtctc                                                                                      >  1:718838/1‑136 (MQ=255)

                                                          acaTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                                              <  2:787348/140‑1 (MQ=255)

                                                           caTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTAt                                                                             >  1:391003/1‑140 (MQ=255)

                                                           caTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTAt                                                                             >  1:1087765/1‑140 (MQ=255)

                                                               gCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                                         <  2:1087765/140‑1 (MQ=255)

                                                                   gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTgg                                                                     >  1:825/1‑140 (MQ=255)

                                                                   gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTgg                                                                     >  2:351750/1‑140 (MQ=255)

                                                                    tggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                                              <  2:702890/130‑1 (MQ=255)

                                                                    tggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                                              >  1:702890/1‑130 (MQ=255)

                                                                    tggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGt                                                                    <  1:79006/140‑1 (MQ=255)

                                                                        cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc                                                                        >  1:342745/1‑132 (MQ=255)

                                                                        cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGc                                                                        <  2:342745/132‑1 (MQ=255)

                                                                        cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtc                                                                >  1:106967/1‑140 (MQ=255)

                                                                        cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtc                                                                >  1:775142/1‑140 (MQ=255)

                                                                         ggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtct                                                               <  2:979771/140‑1 (MQ=255)

                                                                          gcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACACTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtctc                                                              <  2:212548/140‑1 (MQ=255)

                                                                              gATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTg                                                          <  1:351750/140‑1 (MQ=255)

                                                                                    catcatCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                                                    <  1:163841/140‑1 (MQ=255)

                                                                                     atcatcAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtgg                                                   >  1:207868/1‑140 (MQ=255)

                                                                                         tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTggtggtggt                                               >  1:374393/1‑140 (MQ=255)

                                                                                           aGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGttt                                             <  1:1008994/140‑1 (MQ=255)

                                                                                              ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGtt                                          <  2:49906/140‑1 (MQ=255)

                                                                                              ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGtt                                          <  1:720854/140‑1 (MQ=255)

                                                                                              ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGtt                                          <  2:162163/140‑1 (MQ=255)

                                                                                               gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         <  2:207868/140‑1 (MQ=255)

                                                                                                   tACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGCTTGTtctctc                                     <  1:394088/140‑1 (MQ=255)

                                                                                                    aCGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTctctct                                    <  1:57583/140‑1 (MQ=255)

                                                                                                     cGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTAt                                  >  2:832772/1‑140 (MQ=255)

                                                                                                       cAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTc                                <  1:832772/140‑1 (MQ=255)

                                                                                                         aTTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa                               <  2:106967/140‑1 (MQ=255)

                                                                                                         aTTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa                               <  1:522825/140‑1 (MQ=255)

                                                                                                         aTTACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa                               <  2:775142/140‑1 (MQ=255)

                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  1:796154/127‑1 (MQ=255)

                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           >  2:796154/1‑127 (MQ=255)

                                                                                                               tGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              <  1:434715/135‑1 (MQ=255)

                                                                                                               tGCTGGTGTAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              >  2:434715/1‑135 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTTGCCCCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata                     >  1:1002711/1‑140 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCGGATTTAACCCTGCGAATGGATAGCCTCCCGGCATTTATGGGGCTGGGTATCTCTTTGCTGGTGGTGGGTTCTTCTCTCTATTCATTGACTTatat                    >  2:668251/1‑140 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTatat                    >  1:98807/1‑140 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTatat                    >  1:105887/1‑140 (MQ=255)

                                                                                                                        aaCTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATgcgc                >  1:461909/1‑140 (MQ=255)

                                                                                                                         aCTTTAGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGcgcg               <  1:1058667/140‑1 (MQ=255)

                                                                                                                              aGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATgcg                 <  1:832800/133‑1 (MQ=255)

                                                                                                                              aGTCGCCCGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATgcg                 >  2:832800/1‑133 (MQ=255)

                                                                                                                                   cccGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAggg     >  1:173592/1‑140 (MQ=255)

                                                                                                                                    ccGTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTCATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGc    <  2:391003/140‑1 (MQ=255)

                                                                                                                                      gTTTTGC‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCaa  <  2:173592/140‑1 (MQ=255)

TCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCGTTTTG‑‑CCCCCCCTTTTGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAA  >  NC_000913/2601871‑2602143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A