Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,610,133	T→C	R549R (CGT→CGC)	hyfG →	hydrogenase 4 catalytic subunit HyfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,610,133	0	T	C	100.0%	189.6 / NA	53	R549R (CGT→CGC)	hyfG	hydrogenase 4 catalytic subunit HyfG
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (34/19); total (34/19)

GTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTG  >  NC_000913/2609996‑2610268

gTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTC‑AAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAAc                                                                                                                                      >  1:793433/1‑140 (MQ=255)

          cACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTcgccg                                                                                                                             <  1:794749/140‑1 (MQ=255)

           aCCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTcgccg                                                                                                                             >  1:184917/1‑139 (MQ=255)

           aCCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTcgccg                                                                                                                             <  2:184917/139‑1 (MQ=255)

             cGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTg                                                                                                                          >  2:282493/1‑140 (MQ=255)

             cGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTg                                                                                                                          >  1:986994/1‑140 (MQ=255)

                 gATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTAAGGCATTGATCGCAACCGTTCGCCGCTGAAGt                                                                                                                      <  2:247103/140‑1 (MQ=255)

                       cGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCt                                                                                                                           >  1:712021/1‑129 (MQ=255)

                       cGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCt                                                                                                                           <  2:712021/129‑1 (MQ=255)

                          tAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACaga                                                                                                             <  1:17434/140‑1 (MQ=255)

                              cTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGAt                                                                                                         >  2:400793/1‑140 (MQ=255)

                                       tGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGtt                                                                                                >  1:20758/1‑140 (MQ=255)

                                          tACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACt                                                                                             <  1:10792/140‑1 (MQ=255)

                                            cTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGa                                                                                           >  2:286276/1‑140 (MQ=255)

                                             tCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGaa                                                                                          <  2:874216/140‑1 (MQ=255)

                                                 gTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACt                                                                                      >  1:744967/1‑140 (MQ=255)

                                                       accgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATg                                                                                >  1:217925/1‑140 (MQ=255)

                                                       accgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATg                                                                                >  1:227007/1‑140 (MQ=255)

                                                       accgTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATg                                                                                >  1:351181/1‑140 (MQ=255)

                                                          gtgtGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATgcgc                                                                             >  2:634551/1‑140 (MQ=255)

                                                           tgtgACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATgcgcg                                                                            >  1:206205/1‑140 (MQ=255)

                                                           tgtgACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATgcgcg                                                                            >  2:925195/1‑140 (MQ=255)

                                                            gtgACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATgcgcgc                                                                           >  2:951770/1‑140 (MQ=255)

                                                            gtgACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATgcgcgc                                                                           <  2:206205/140‑1 (MQ=255)

                                                                  cTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTAAAGTTACTGAAAACTATTATGCGCGCCGGAAc                                                                     >  1:346215/1‑140 (MQ=255)

                                                                     gTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACcgc                                                                  <  1:286276/140‑1 (MQ=255)

                                                                      tAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACcgcg                                                                 <  1:634551/140‑1 (MQ=255)

                                                                      tAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACcgcg                                                                 <  1:925195/140‑1 (MQ=255)

                                                                       aGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGa                                                                <  2:227007/140‑1 (MQ=255)

                                                                         aTGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACg                                                              <  2:744967/140‑1 (MQ=255)

                                                                             gcgcGAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGa                                                          >  1:750607/1‑140 (MQ=255)

                                                                             gcgcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGa                                                          >  2:54073/1‑140 (MQ=255)

                                                                             gcgcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGa                                                          >  1:747450/1‑140 (MQ=255)

                                                                              cgcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGaa                                                         >  2:602535/1‑140 (MQ=255)

                                                                               gcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGaaa                                                        >  1:693248/1‑140 (MQ=255)

                                                                               gcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGaaa                                                        >  1:344999/1‑140 (MQ=255)

                                                                               gcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGaaa                                                        >  1:902553/1‑140 (MQ=255)

                                                                               gcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGaaa                                                        >  1:261671/1‑140 (MQ=255)

                                                                                           cAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTcgcg                                            <  2:261671/140‑1 (MQ=255)

                                                                                           cAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTcgcg                                            <  2:217925/140‑1 (MQ=255)

                                                                                                      ccGTATAAAGAGATCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGt                                 <  2:793433/140‑1 (MQ=255)

                                                                                                         tataAAGAGATCGAACGCTACGGCATAGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAg                              >  2:915955/1‑140 (MQ=255)

                                                                                                                agaTCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGc                       >  2:278591/1‑140 (MQ=255)

                                                                                                                   tCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTg                                      >  2:196649/1‑122 (MQ=255)

                                                                                                                   tCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTg                                      <  1:196649/122‑1 (MQ=255)

                                                                                                                   tCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGt                                 <  2:319327/127‑1 (MQ=255)

                                                                                                                   tCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGt                                 >  1:319327/1‑127 (MQ=255)

                                                                                                                   tCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCttt                    >  2:597502/1‑140 (MQ=255)

                                                                                                                   tCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCttt                    >  2:820464/1‑140 (MQ=255)

                                                                                                                   tCGAACGCTACGGCATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCttt                    >  2:223095/1‑140 (MQ=255)

                                                                                                                                cATTGATCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCgg       <  2:344999/140‑1 (MQ=255)

                                                                                                                                     aTCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTg  >  2:444074/1‑140 (MQ=255)

                                                                                                                                     aTCGCAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTg  >  2:958902/1‑140 (MQ=255)

GTTTCTGACGCACCGCTGATTATCGGTAGCCTTGATCCCTGCTACTCCTGTACCGACCGTGTGACGCTGGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTG  >  NC_000913/2609996‑2610268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A