Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,753	G→A	S280S (AGC→AGT)	yqeF ←	putative acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,753	0	G	A	100.0%	71.9 / NA	22	S280S (AGC→AGT)	yqeF	putative acyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (5/17); total (5/17)

AAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACGCTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTAT  >  NC_000913/2984621‑2984886

aaGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCCGCGCCAATCCCCTCAATGCCCGAACTTCACCAACACCGGcca                                                                                                                                >  2:305716/1‑138 (MQ=255)

      cTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATgcgc                                                                                                                          <  1:429447/140‑1 (MQ=255)

       tgtgCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCCGCGCCATTCCCCTCAATGCCCGGTCTACACCAACACTGGCAAAAGcgcg                                                                                                                         >  2:621199/1‑140 (MQ=255)

           cagcaAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATg                                                                                                                     <  1:305716/140‑1 (MQ=255)

            agcaAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGc                                                                                                                    <  2:964272/140‑1 (MQ=255)

                aaCGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCGGCGCAATTTCCCTCAATGCCGGGTATACACCAACACTGGCAAATGCGCGAATGCgggg                                                                                                                >  2:64064/1‑139 (MQ=255)

                aaCGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCCGCGCAATTCCCATCAAAGCCGGATCTACACCAACACCGGCAAAAGCGCGAATGCggac                                                                                                                >  2:874414/1‑138 (MQ=255)

                   gCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCCGCGCCAATCCCCTCAATGCCCGAGCTACACCAACACCGGCAAAAACGCGGATGGGGGGCAg                                                                                                             >  2:340732/1‑140 (MQ=255)

                     ttCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCa                                                                                                           <  1:141327/140‑1 (MQ=255)

                     ttCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCa                                                                                                           <  1:1068131/140‑1 (MQ=255)

                     ttCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGACCAGCa                                                                                                           <  1:874414/140‑1 (MQ=255)

                        aTTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCCTACACCCGCGCCATTCCCATCAAAGCCGGGGCTACACCCACACCCGCAAAAACGCGAAAGCGGGCCaccccag                                                                                                        >  2:875449/1‑134 (MQ=255)

                                  aTAAGATCGACTTCAGTCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACAACGGCGCCATTTCCATCAAATCCGGATCTACACCAACACTGGCAAATGCGCGGATGCGGGGCAGCCCGGGGAAAATCaa                                                                                              >  2:786003/1‑140 (MQ=255)

                                                         ccAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCtca                                                                       <  1:93018/140‑1 (MQ=255)

                                                         ccAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCtca                                                                       <  2:270152/140‑1 (MQ=255)

                                                         ccAGCCTACACGCTACAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCtca                                                                       <  2:160834/140‑1 (MQ=255)

                                                          cAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCtcat                                                                      <  1:64064/140‑1 (MQ=255)

                                                           aGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCtcatc                                                                     <  2:500515/140‑1 (MQ=255)

                                                                acacGCTCCAGGCAACGGCGGGTCGCATACACCCGCGCCAATCCCCTCCAAGCCCGATCTACACCCACACCCGCAAAAGCGCGGAAGCGGGCCCGCACGGGGAAAATCAACACCCGCGCTTTTGCTTCGCCcctcatcat                                                                >  2:840922/1‑140 (MQ=255)

                                                                   cGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTAc                                                             <  1:462324/140‑1 (MQ=255)

                                                                        cAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAg                                                        <  1:621199/140‑1 (MQ=255)

                                                                           gCAACGGCGGGTCGCATAAACCCGCGCCAATCCCCTCAATGCCGGGTCTACACCAACACCGGCAAATGCGCGAAAGCGGGCCAGCACGGGGAAATTTAAAGCTCGCGCTTTTGGTTTGCTCATCATCATTACCCCAGCTg                                                     >  2:462324/1‑140 (MQ=255)

                                                                                              acCGGCGCAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGgatga                                  >  1:848672/1‑140 (MQ=255)

                                                                                                  gcgcAATTCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCa                              >  2:198670/1‑140 (MQ=255)

                                                                                                     cAATTCCCATCCATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTa                           <  2:4175/140‑1 (MQ=255)

                                                                                                         tCCCATCAATGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCg                       <  1:198670/140‑1 (MQ=255)

                                                                                                                  tGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAg              >  1:762931/1‑140 (MQ=255)

                                                                                                                  tGCCGGATCTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAg              >  1:75041/1‑140 (MQ=255)

                                                                                                                          cTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCgaga      <  2:75041/140‑1 (MQ=255)

                                                                                                                          cTACACCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCgaga      <  1:413543/140‑1 (MQ=255)

                                                                                                                            acacCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATCACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACt    >  1:205012/1‑140 (MQ=255)

                                                                                                                              acCAACACTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTAt  <  2:848672/140‑1 (MQ=255)

AAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCCCATCAATGCCGGATCTACACCAACGCTGGCAAATGCGCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTAT  >  NC_000913/2984621‑2984886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A