Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,157	T→C	intergenic (‑77/+16)	yggE ← / ← argO	uncharacterized protein YggE/L‑arginine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,157	0	T	C	100.0%	192.0 / NA	54	intergenic (‑77/+16)	yggE/argO	uncharacterized protein YggE/L‑arginine exporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/27); total (27/27)

TGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACG  >  NC_000913/3068022‑3068292

tGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCa                                                                                                                                     <  2:136338/140‑1 (MQ=255)

 gcgcTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCAt                                                                                                                                    <  2:163850/140‑1 (MQ=255)

       ccATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATa                                                                                                                              >  1:338216/1‑140 (MQ=255)

             cGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCt                                                                                                                        >  1:197407/1‑140 (MQ=255)

               cTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTaa                                                                                                                      <  1:609436/140‑1 (MQ=255)

                 aaTACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACt                                                                                                                    >  1:141298/1‑140 (MQ=255)

                        aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAgcg                                                                                                                           >  2:862433/1‑126 (MQ=255)

                        aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAgcg                                                                                                                           <  1:862433/126‑1 (MQ=255)

                         ttAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGACAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAgg                                                                                                            >  1:967620/1‑140 (MQ=255)

                          tAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGc                                                                                                           <  2:203739/140‑1 (MQ=255)

                            aTGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCtt                                                                                                         >  1:1111466/1‑140 (MQ=255)

                                cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                     >  2:996499/1‑140 (MQ=255)

                                cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                     >  2:659466/1‑140 (MQ=255)

                                cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                     >  1:500327/1‑140 (MQ=255)

                                cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                     <  2:213024/140‑1 (MQ=255)

                                cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                     <  1:395735/140‑1 (MQ=255)

                                            aaCTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATAcc                                                                                         >  1:403654/1‑140 (MQ=255)

                                              cTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGt                                                                                       <  1:659466/140‑1 (MQ=255)

                                                 tGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGtctc                                                                                    >  1:183560/1‑140 (MQ=255)

                                                 tGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGACCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGAGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGtctc                                                                                    >  2:1000453/1‑140 (MQ=255)

                                                      ttCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                               <  2:357316/140‑1 (MQ=255)

                                                      ttCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                               <  1:16044/140‑1 (MQ=255)

                                                         aCGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGt                                                                                       <  2:271094/129‑1 (MQ=255)

                                                         aCGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGt                                                                                       >  1:271094/1‑129 (MQ=255)

                                                                     ccGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                <  2:1111466/140‑1 (MQ=255)

                                                                       gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                              <  1:706642/140‑1 (MQ=255)

                                                                       gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                              <  2:197407/140‑1 (MQ=255)

                                                                         gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                >  2:405386/1‑136 (MQ=255)

                                                                         gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                <  1:405386/136‑1 (MQ=255)

                                                                           tAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcac                                                          <  1:34268/140‑1 (MQ=255)

                                                                                 aCTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaca                                                    >  1:671164/1‑140 (MQ=255)

                                                                                  cTTGCCCAAAATTCATGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                   <  2:651612/140‑1 (MQ=255)

                                                                                  cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                   >  2:870967/1‑140 (MQ=255)

                                                                                     gCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATc                                                >  1:86645/1‑140 (MQ=255)

                                                                                      cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                               >  2:609536/1‑140 (MQ=255)

                                                                                      cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                               >  2:452192/1‑140 (MQ=255)

                                                                                            aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                         <  1:1000453/140‑1 (MQ=255)

                                                                                            aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                         <  2:338216/140‑1 (MQ=255)

                                                                                                 ggACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                    <  2:671164/140‑1 (MQ=255)

                                                                                                       ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATaa                              >  1:144746/1‑140 (MQ=255)

                                                                                                       ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATaa                              >  1:95570/1‑140 (MQ=255)

                                                                                                            ggCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACTGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                         <  2:155766/140‑1 (MQ=255)

                                                                                                            ggCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                         <  1:456463/140‑1 (MQ=255)

                                                                                                            ggCAAGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACAGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                         <  1:996499/140‑1 (MQ=255)

                                                                                                               aaGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                    >  2:935199/1‑126 (MQ=255)

                                                                                                               aaGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                    <  1:935199/126‑1 (MQ=255)

                                                                                                               aaGCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                      >  2:33170/1‑140 (MQ=255)

                                                                                                                 gCTTAGCGCCTCTGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGc                    >  1:512028/1‑140 (MQ=255)

                                                                                                                           tctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGcc             >  2:818134/1‑137 (MQ=255)

                                                                                                                           tctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGcc             <  1:818134/137‑1 (MQ=255)

                                                                                                                           tctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTg          >  2:436835/1‑140 (MQ=255)

                                                                                                                           tctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTg          >  2:155070/1‑140 (MQ=255)

                                                                                                                           tctGTTTTATTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTg          <  1:436835/140‑1 (MQ=255)

                                                                                                                                   aTTTCTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGAcg  <  1:452192/140‑1 (MQ=255)

TGCGCTGCCATCCCGCTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACG  >  NC_000913/3068022‑3068292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A