Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,085,636	G→A	F92F (TTC→TTT)	speA ←	arginine decarboxylase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,085,636	0	G	A	100.0%	225.3 / NA	63	F92F (TTC→TTT)	speA	arginine decarboxylase, biosynthetic
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/32); total (31/32)

CTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGGAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAG  >  NC_000913/3085504‑3085771

cTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAaacaga                                                                                                                                  <  2:563937/140‑1 (MQ=255)

 tCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAaacagaa                                                                                                                                 >  1:349251/1‑140 (MQ=255)

 tCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAaacagaa                                                                                                                                 <  1:772179/140‑1 (MQ=255)

 tCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAaacagaa                                                                                                                                 <  1:79012/140‑1 (MQ=255)

 tCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAaacagaa                                                                                                                                 <  1:863877/140‑1 (MQ=255)

 tCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAaacagaa                                                                                                                                 <  2:1094388/140‑1 (MQ=255)

 tCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAaacagaa                                                                                                                                 <  2:225305/140‑1 (MQ=255)

   aaTCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAaacagaaca                                                                                                                               >  1:410084/1‑140 (MQ=255)

   aaTCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAaacagaaca                                                                                                                               >  1:541044/1‑140 (MQ=255)

      cACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTg                                                                                                                            <  2:574968/140‑1 (MQ=255)

      cACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTg                                                                                                                            <  2:656314/140‑1 (MQ=255)

      cACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTg                                                                                                                            <  1:997515/140‑1 (MQ=255)

            gcggTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTgcaggca                                                                                                                      <  1:188958/140‑1 (MQ=255)

             cggTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTgcaggcag                                                                                                                     >  1:626556/1‑140 (MQ=255)

               gTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTATGGAAaacaga                                                                                                                                  <  2:902680/125‑1 (MQ=255)

               gTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTATGGAAaacaga                                                                                                                                  >  1:902680/1‑125 (MQ=255)

                 gCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGc                                                                                                                 <  2:772286/140‑1 (MQ=255)

                  cTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCt                                                                                                                >  1:113755/1‑140 (MQ=255)

                       tAACTTTGATCGGATAAACAAGGAGGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGccc                                                                                                           <  1:236499/140‑1 (MQ=255)

                          cTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCtgt                                                                                                        >  1:840253/1‑140 (MQ=255)

                              gATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCtt                                                                                                    <  1:12702/140‑1 (MQ=255)

                               aTCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTc                                                                                                   >  2:422055/1‑140 (MQ=255)

                                    aTAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAg                                                                                              <  2:349251/140‑1 (MQ=255)

                                    aTAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAg                                                                                              <  1:939557/140‑1 (MQ=255)

                                    aTAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAg                                                                                              <  2:541044/140‑1 (MQ=255)

                                            ggAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCAcc                                                                                      <  2:840253/140‑2 (MQ=255)

                                             gAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTa                                                                                     >  2:757222/1‑140 (MQ=255)

                                              aaGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCGGACGCTGGCCCTGTGCTTCACGAGTTTTCACTaa                                                                                    >  1:37730/1‑140 (MQ=255)

                                              aaGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTaa                                                                                    >  1:271343/1‑140 (MQ=255)

                                              aaGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTaa                                                                                    >  2:507919/1‑140 (MQ=255)

                                                  aaTCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTgc                                                                                >  1:817630/1‑140 (MQ=255)

                                                            ataGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGACCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGa                                                                      >  1:47321/1‑140 (MQ=255)

                                                               gCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGAcgc                                                                   <  2:817630/140‑1 (MQ=255)

                                                                ccGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGAcgcg                                                                  <  1:422055/140‑1 (MQ=255)

                                                                ccGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGACCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGAcgcg                                                                  <  2:47321/140‑1 (MQ=255)

                                                                  gTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAg                                                                >  2:319905/1‑140 (MQ=255)

                                                                         tCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCgg                                                         >  1:1011682/1‑140 (MQ=255)

                                                                          cccTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCggg                                                        <  1:757222/140‑1 (MQ=255)

                                                                             tCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACg                                                     >  2:758996/1‑140 (MQ=255)

                                                                              cGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACgt                                                    <  1:507919/140‑1 (MQ=255)

                                                                              cGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACgt                                                    >  1:610007/1‑140 (MQ=255)

                                                                              cGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACgt                                                    >  1:67724/1‑140 (MQ=255)

                                                                               gCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACgtc                                                   <  2:665967/140‑1 (MQ=255)

                                                                                cACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACgtcc                                                  >  2:175276/1‑140 (MQ=255)

                                                                                     ttGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACgtccgggtc                                             >  1:765330/1‑140 (MQ=255)

                                                                                      tGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACgtccgggtcc                                            >  1:784113/1‑140 (MQ=255)

                                                                                          cgcgGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGc                                        <  2:765330/140‑1 (MQ=255)

                                                                                           gcggcgTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGca                                       <  2:67724/140‑1 (MQ=255)

                                                                                           gcggcgTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGca                                       <  2:1011682/140‑1 (MQ=255)

                                                                                               cgTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGc                                        <  2:273416/135‑1 (MQ=255)

                                                                                               cgTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGc                                        >  1:273416/1‑135 (MQ=255)

                                                                                                         aCGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGc                         >  1:1062701/1‑140 (MQ=255)

                                                                                                          cGCAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGcc                        >  1:191247/1‑140 (MQ=255)

                                                                                                            cAAACGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCa                      >  2:1092146/1‑140 (MQ=255)

                                                                                                              aaCGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCGGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGCCGTCCGGGTCCGGGCACACGCTAATGTGGCCCAgg                    <  2:37730/140‑2 (MQ=255)

                                                                                                              aaCGGTGCTGCAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGc                    >  2:1032822/1‑140 (MQ=255)

                                                                                                                     ctgcAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTaa             <  1:1052605/140‑1 (MQ=255)

                                                                                                                     ctgcAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTaa             <  2:1085220/140‑1 (MQ=255)

                                                                                                                       gcAGGATCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACg           <  1:14564/140‑1 (MQ=255)

                                                                                                                             tCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCAtag     <  1:319905/140‑1 (MQ=255)

                                                                                                                             tCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCAtag     >  2:325601/1‑140 (MQ=255)

                                                                                                                             tCTGTGGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCAtag     >  1:817317/1‑140 (MQ=255)

                                                                                                                                gtgGAAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTGCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCAtagtag  >  2:829340/1‑140 (MQ=255)

                                                                                                                                gtgGAAAACAGAACAGTGCAGGCAGACGCTGGCCCCGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCAtagtag  >  2:112718/1‑140 (MQ=255)

CTCAATCACGCGGCGGTGCTGGTTAACTTTGATCGGATAAACAAGGAAGTAATCGCCGTTATAGCCGTAGGATTCCCTCGCACGTTTGAACGCGGCGTTAATGGAACGCAAACGGTGCTGCAGGATCTGTGGGAAACAGAACAGTGCAGGCAGACGCTGGCCCTGTGCTTCACGAGTTTTCACTAACTGCGCGAGATCGACGCGAGCTTCCGGGACGTCCGGGTCCGGGCACACGCTAATGTGGCCCAGCTCGTTAACGTCATAGTAG  >  NC_000913/3085504‑3085771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A