Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,411,633	G→A	intergenic (+66/‑20)	fis → / → yhdJ	DNA‑binding transcriptional dual regulator Fis/DNA adenine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,411,633	0	G	A	98.7%	259.2 / ‑4.6	76	intergenic (+66/‑20)	fis/yhdJ	DNA‑binding transcriptional dual regulator Fis/DNA adenine methyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (37/38); total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

TGCTGCGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTG  >  NC_000913/3411498‑3411767

tgctgcGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGatgt                                                                                                                                     >  1:1059835/1‑139 (MQ=255)

tgctgcGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGatgt                                                                                                                                     <  2:1059835/139‑1 (MQ=255)

tgctgcGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTc                                                                                                                                    >  1:542352/1‑140 (MQ=255)

tgctgcGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTc                                                                                                                                    >  2:97/1‑140 (MQ=255)

tgctgcGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTc                                                                                                                                    >  1:695645/1‑140 (MQ=255)

tgctgcGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTc                                                                                                                                    >  2:353711/1‑140 (MQ=255)

    gcgcTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACaa                                                                                                                                <  1:509102/140‑1 (MQ=255)

     cgcTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACaaa                                                                                                                               >  2:377161/1‑140 (MQ=255)

     cgcTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACaaa                                                                                                                               >  1:636147/1‑140 (MQ=255)

         gatgatGGGCATCAACCGTGGTACTCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGa                                                                                                                           <  2:906110/140‑1 (MQ=255)

         gatgatGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGa                                                                                                                           <  2:887665/140‑1 (MQ=255)

         gatgatGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGa                                                                                                                           <  2:860627/140‑1 (MQ=255)

         gatgatGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGa                                                                                                                           <  2:312594/140‑1 (MQ=255)

         gatgatGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGa                                                                                                                           <  1:460817/140‑1 (MQ=255)

          atgatgGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAg                                                                                                                          <  2:994414/140‑1 (MQ=255)

            gatgGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTg                                                                                                                        <  2:94611/140‑1 (MQ=255)

              tgGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAGGGGAGTGAc                                                                                                                      >  2:225610/1‑140 (MQ=255)

               gggCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGAcc                                                                                                                     >  2:322176/1‑140 (MQ=255)

                  cATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATg                                                                                                                  >  2:1063186/1‑140 (MQ=255)

                  cATCAACCGTGGTACGCTGCGTAAAAAAATGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATg                                                                                                                  <  1:563344/140‑1 (MQ=255)

                    tCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGAcc                                                                                                                     >  1:178504/1‑135 (MQ=255)

                    tCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGAcc                                                                                                                     <  2:178504/135‑1 (MQ=255)

                    tCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCAt                                                                                                                   >  1:117368/1‑137 (MQ=255)

                    tCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCAt                                                                                                                   <  2:117368/137‑1 (MQ=255)

                       aCCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAAc                                                                                                             <  1:201068/140‑1 (MQ=255)

                       aCCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAAc                                                                                                             <  2:1031481/140‑1 (MQ=255)

                                cGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGa                                                                                                    >  2:418658/1‑140 (MQ=255)

                                  cTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAAc                                                                                                  >  1:735618/1‑140 (MQ=255)

                                   tGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAAcc                                                                                                 >  2:740188/1‑140 (MQ=255)

                                     cGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGa                                                                                               >  2:843259/1‑140 (MQ=255)

                                     cGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGa                                                                                               >  2:302779/1‑140 (MQ=255)

                                     cGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGa                                                                                               >  1:977675/1‑140 (MQ=255)

                                       tAAAAAATTGAAAAAATACGGCATGAACTAATTCGGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGAcc                                                                                             <  1:351/140‑1 (MQ=255)

                                       tAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGAcc                                                                                             <  2:542352/140‑1 (MQ=255)

                                       tAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGAcc                                                                                             <  1:175759/140‑1 (MQ=255)

                                       tAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGAcc                                                                                             >  2:590185/1‑140 (MQ=255)

                                       tAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGAcc                                                                                             <  1:418658/140‑1 (MQ=255)

                                         aaaaaTTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCg                                                                                           <  1:97/140‑1 (MQ=255)

                                          aaaaTTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCgg                                                                                          <  2:205068/140‑1 (MQ=255)

                                            aaTTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGtt                                                                                        <  1:377161/140‑1 (MQ=255)

                                                    aaaTACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAGGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATg                                                                                <  1:225610/140‑1 (MQ=255)

                                                           gCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTaa                                                                         <  1:740188/140‑1 (MQ=255)

                                                           gCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTaa                                                                         <  2:636147/140‑1 (MQ=255)

                                                             aTGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGa                                                                       <  2:1063407/140‑1 (MQ=255)

                                                               gAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGAcc                                                                     >  1:1042778/1‑140 (MQ=255)

                                                               gAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGAcc                                                                     <  1:502364/140‑1 (MQ=255)

                                                                aaCTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCa                                                                    <  2:1042778/140‑1 (MQ=255)

                                                                     aTTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCattatt                                                               >  2:827853/1‑140 (MQ=255)

                                                                       tCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCa                                                             <  2:205291/140‑1 (MQ=255)

                                                                       tCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCAACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGATAATGAAGCTAAGACCATTATTCa                                                             <  2:560673/140‑1 (MQ=255)

                                                                          ggTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACgg                                                          >  2:42145/1‑140 (MQ=255)

                                                                           gTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGt                                                         >  2:76492/1‑140 (MQ=255)

                                                                           gTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGt                                                         >  2:472083/1‑140 (MQ=255)

                                                                            ttAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTg                                                        >  2:354062/1‑140 (MQ=255)

                                                                               gCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCTGTTTGGTAATGAAGCTAAGACCATTATTc                                                              >  2:947902/1‑131 (MQ=255)

                                                                               gCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTc                                                              <  1:947902/131‑1 (MQ=255)

                                                                               gCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATg                                                     >  2:140883/1‑140 (MQ=255)

                                                                                     tGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTg                                               >  1:26729/1‑140 (MQ=255)

                                                                                        ttGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCg                                            <  1:1070125/140‑1 (MQ=255)

                                                                                        ttGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCg                                            <  1:827853/140‑1 (MQ=255)

                                                                                                     cgcTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGAt                               <  1:590185/140‑1 (MQ=255)

                                                                                                      gcTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATc                              >  1:191517/1‑140 (MQ=255)

                                                                                                      gcTACTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATc                              >  2:740476/1‑140 (MQ=255)

                                                                                                         aCTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATcccc                           <  1:353711/140‑1 (MQ=255)

                                                                                                          cTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCccg                          >  2:403924/1‑140 (MQ=255)

                                                                                                          cTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCccg                          >  2:83915/1‑140 (MQ=255)

                                                                                                          cTCGGCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCccg                          >  1:311913/1‑140 (MQ=255)

                                                                                                              gCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGa                      <  1:76492/140‑1 (MQ=255)

                                                                                                              gCATGGGGAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGa                      <  1:302779/140‑1 (MQ=255)

                                                                                                                  ggggAAGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAgt                  >  1:509994/1‑140 (MQ=255)

                                                                                                                       aGCGCCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCga             >  1:725381/1‑140 (MQ=255)

                                                                                                                         cgcTTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCgatc           <  2:586449/140‑1 (MQ=255)

                                                                                                                         cgcCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCgatc           >  2:271208/1‑140 (MQ=255)

                                                                                                                         cgcCTTTTTTATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCgatc           >  2:1086983/1‑140 (MQ=255)

                                                                                                                             ttttttATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCgatctgat       <  1:472083/140‑1 (MQ=255)

                                                                                                                             ttttttATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCgatctgat       >  1:340494/1‑140 (MQ=255)

                                                                                                                              tttttATAGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCgatctgatc      <  1:83915/140‑1 (MQ=255)

                                                                                                                                  tataGATGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTg  <  1:740476/140‑1 (MQ=255)

TGCTGCGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATGAGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATGCGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTG  >  NC_000913/3411498‑3411767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A