Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,577,648	A→G	intergenic (‑56/+83)	gntK ← / ← gntR	D‑gluconate kinase, thermostable/DNA‑binding transcriptional repressor GntR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,577,648	0	A	G	100.0%	156.0 / NA	45	intergenic (‑56/+83)	gntK/gntR	D‑gluconate kinase, thermostable/DNA‑binding transcriptional repressor GntR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/30); total (15/30)

CACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTG  >  NC_000913/3577514‑3577783

cACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTg                                                                                                                                    <  2:734668/140‑1 (MQ=255)

  cTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTc                                                                                                                                  <  1:1064474/140‑1 (MQ=255)

        aCCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTc                                                                                                                                  <  1:780583/134‑1 (MQ=255)

        aCCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTc                                                                                                                                  >  2:780583/1‑134 (MQ=255)

        aCCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGc                                                                                                                              >  2:583017/1‑138 (MQ=255)

        aCCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGc                                                                                                                              <  1:583017/138‑1 (MQ=255)

        aCCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCg                                                                                                                            >  2:226124/1‑140 (MQ=255)

         ccGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCgg                                                                                                                           <  1:131808/140‑1 (MQ=255)

          cGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCGCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGa                                                                                                                          >  2:214329/1‑140 (MQ=255)

          cGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGa                                                                                                                          >  2:43467/1‑140 (MQ=255)

           gCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGAc                                                                                                                         <  1:902145/140‑1 (MQ=255)

            cAGATTAGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACa                                                                                                                        <  1:993558/140‑1 (MQ=255)

             aGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACaa                                                                                                                       <  2:21377/140‑1 (MQ=255)

             aGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACaa                                                                                                                       <  1:116085/140‑1 (MQ=255)

                 ttGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATc                                                                                                                   <  2:1026815/140‑1 (MQ=255)

                   gCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAg                                                                                                                 <  1:226124/140‑1 (MQ=255)

                    ccGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGa                                                                                                                <  1:915771/140‑1 (MQ=255)

                      gCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAg                                                                                                              <  1:996719/140‑1 (MQ=255)

                         gCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCaa                                                                                                           >  2:379470/1‑140 (MQ=255)

                                 cGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATatccatc                                                                                                   <  1:16713/140‑1 (MQ=255)

                                 cGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATatccatc                                                                                                   <  1:497171/140‑1 (MQ=255)

                                   cccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATAtccatcca                                                                                                 <  1:763046/140‑1 (MQ=255)

                                    ccATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATccatccat                                                                                                >  2:436779/1‑140 (MQ=255)

                                       tCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGcc                                                                                             <  2:310412/140‑1 (MQ=255)

                                         aaGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                                           <  2:926983/140‑1 (MQ=255)

                                         aaGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                                           <  1:999912/140‑1 (MQ=255)

                                         aaGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                                           <  1:379470/140‑1 (MQ=255)

                                         aaGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                                           <  1:305446/140‑1 (MQ=255)

                                            aCGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                                           >  2:803/1‑137 (MQ=255)

                                            aCGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCtt                                                                                           <  1:803/137‑1 (MQ=255)

                                                       gtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAg                                                                                        >  1:226384/1‑129 (MQ=255)

                                                       gtgATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAg                                                                                        <  2:226384/129‑1 (MQ=255)

                                                               ggTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACt                                                                     >  1:795491/1‑140 (MQ=255)

                                                                        tGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtg                                                            <  1:963072/140‑1 (MQ=255)

                                                                          cTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTtgtg                                                          >  1:662777/1‑140 (MQ=255)

                                                                             aaaGTGGTGCCGCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGc                                                       <  1:214329/140‑1 (MQ=255)

                                                                             aaaGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGc                                                       <  1:705130/140‑1 (MQ=255)

                                                                               aGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCtt                                                     >  1:607463/1‑140 (MQ=255)

                                                                                   gtgCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAAt                                                 >  2:40676/1‑140 (MQ=255)

                                                                                   gtgCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAAt                                                 >  1:162107/1‑140 (MQ=255)

                                                                                        cccACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATc                                            <  1:43467/140‑1 (MQ=255)

                                                                                                   aGAATTAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGAc                                 <  2:795491/140‑1 (MQ=255)

                                                                                                        tAAAAATGTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGt                            <  2:607463/140‑1 (MQ=255)

                                                                                                               gTTACGGGTAACTGTTATCGGTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCt                     <  2:162107/140‑1 (MQ=255)

                                                                                                                                  ggTAGCATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGgtg  >  1:203820/1‑140 (MQ=255)

CACTGGCGACCGCAGATTTGCCGCTGCCCGATACGCCCATCAAGACGTAAATGTGGTGATCATGGTTAGTCGTGCTCAAAGTGGTGCCCCCACAATACAAGAATTAAAAATGTTACGGGTAACTGTTATCGGTAACATTGTCCAGCCGGACAATATCAGAAGCAATATCCATCCATGCCTTAAGTGTATAAGTGTGAGCTACTTCAAATTTGTGGGCTTAAATAGATCCGCCCGGTGACAAGGTGAAACCTAAATCTAACATTTTCGGTG  >  NC_000913/3577514‑3577783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A