Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,588,643	A→G	L180L (TTG→CTG)	ugpC ←	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,588,643	0	A	G	100.0%	147.9 / NA	42	L180L (TTG→CTG)	ugpC	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/15); total (27/15)

TCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCT  >  NC_000913/3588510‑3588765

tCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTg                                                                                                                      <  1:437243/140‑1 (MQ=255)

          cAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGa                                                                                                            >  2:86168/1‑140 (MQ=255)

              tGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCa                                                                                                        <  1:924599/140‑1 (MQ=255)

                  gtgCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTg                                                                                                    >  2:289441/1‑140 (MQ=255)

                  gtgCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGCTCAAGACGCAtgtg                                                                                                    >  2:771995/1‑140 (MQ=255)

                   tgCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGc                                                                                                   >  2:411504/1‑140 (MQ=255)

                        aTCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCAcgcg                                                                                              <  1:771995/140‑1 (MQ=255)

                          cTGTTCGGCAACACCGCCGTTCATCACCGTTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCa                                                                                            >  2:666584/1‑140 (MQ=255)

                           tGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAg                                                                                           >  2:1104963/1‑140 (MQ=255)

                            gTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGc                                                                                          <  1:1104963/140‑1 (MQ=255)

                             ttCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCt                                                                                         >  1:1040063/1‑140 (MQ=255)

                             ttCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCt                                                                                         <  1:225824/140‑1 (MQ=255)

                               cGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTg                                                                                       >  2:120106/1‑140 (MQ=255)

                                   aaCACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCAt                                                                                   <  1:581659/140‑1 (MQ=255)

                                     cacCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCg                                                                                 <  1:666584/140‑1 (MQ=255)

                                                atcaCCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTagagag                                                                      >  2:1139204/1‑140 (MQ=255)

                                                       ttACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCtca                                                               >  1:604814/1‑140 (MQ=255)

                                                            cGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCaaa                                                          >  1:1177704/1‑140 (MQ=255)

                                                               tGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAg                                                       >  2:1020993/1‑140 (MQ=255)

                                                                  gCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGaa                                                    <  2:583803/140‑1 (MQ=255)

                                                                       cGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACAccg                                               <  1:999371/140‑1 (MQ=255)

                                                                            tCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGa                                          <  1:86168/140‑1 (MQ=255)

                                                                             cGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGAt                                         <  1:1139204/140‑1 (MQ=255)

                                                                                 tCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATcgcg                                     <  1:411504/140‑1 (MQ=255)

                                                                                     ccTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGcaca                                 >  1:508752/1‑140 (MQ=255)

                                                                                          tCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGa                                          >  1:113373/1‑126 (MQ=255)

                                                                                          tCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGa                                          <  2:113373/126‑1 (MQ=255)

                                                                                          tCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATcgc                            >  2:1214819/1‑140 (MQ=255)

                                                                                           cGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATcgcg                           <  2:604814/140‑1 (MQ=255)

                                                                                                 tAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGccc                     >  1:944102/1‑140 (MQ=255)

                                                                                                    cGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATc                  >  2:647352/1‑140 (MQ=255)

                                                                                                    cGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATc                  >  2:955074/1‑140 (MQ=255)

                                                                                                      tAGAGTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGc                >  2:685461/1‑140 (MQ=255)

                                                                                                         agTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCcac             >  1:1210997/1‑140 (MQ=255)

                                                                                                         agTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCACAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCcac             >  2:909141/1‑140 (MQ=255)

                                                                                                          gTGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCcaca            >  1:973616/1‑140 (MQ=255)

                                                                                                           tGAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCATGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCacac           >  2:832909/1‑140 (MQ=255)

                                                                                                            gAAGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACg          <  1:1214819/140‑1 (MQ=255)

                                                                                                             aaGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGc         >  1:957559/1‑140 (MQ=255)

                                                                                                             aaGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGc         >  2:699524/1‑140 (MQ=255)

                                                                                                              aGTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCt        >  2:123111/1‑140 (MQ=255)

                                                                                                               gTCGTTTTCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTg       >  1:862262/1‑140 (MQ=255)

                                                                                                                    tttCAGGCGACGGTGCAGCTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCt  <  1:1020993/140‑1 (MQ=255)

TCGTAGACTTCAACTGGTGTGCCAATCTGTTCGGCAACACCGCCGTTCATCACCATTACTCGCTGGGCGAGCGTCATCGCTTCAACCTGATCGTGAGTAACGTAGAGTGAAGTCGTTTTCAGGCGACGGTGCAACTGTTGCAGTTCAAGACGCATCTGCACGCGCAGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCT  >  NC_000913/3588510‑3588765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A