Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,878,864	T→C	E419G (GAA→GGA)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,878,864	0	T	C	100.0%	111.9 / NA	32	E419G (GAA→GGA)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/17); total (15/17)

GAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCG  >  NC_000913/3878732‑3878992

gagagCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAg                                                                                                                           >  1:400024/1‑140 (MQ=255)

 agagCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAgc                                                                                                                          >  2:751535/1‑140 (MQ=255)

  gagCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAgcg                                                                                                                         >  2:984585/1‑140 (MQ=255)

    gCATATTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGcc                                                                                                                       <  1:280182/140‑1 (MQ=255)

     cATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCg                                                                                                                      <  1:948670/140‑1 (MQ=255)

             cGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGt                                                                                                              <  2:905933/140‑1 (MQ=255)

               aaGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTc                                                                                                            >  1:1030716/1‑140 (MQ=255)

               aaGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTc                                                                                                            >  2:163676/1‑140 (MQ=255)

                 gcgcgcTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCt                                                                                                          >  2:962975/1‑140 (MQ=255)

                  cgcgcTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTcc                                                                                                           <  1:519076/138‑1 (MQ=255)

                  cgcgcTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTcc                                                                                                           >  2:519076/1‑138 (MQ=255)

                  cgcgcTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTg                                                                                                         >  2:41063/1‑140 (MQ=255)

                               cGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGt                                                                                            >  2:842063/1‑140 (MQ=255)

                                          ttACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAg                                                                                 <  1:355754/140‑1 (MQ=255)

                                          ttACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAg                                                                                 <  1:41063/140‑1 (MQ=255)

                                            aCCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGc                                                                               <  1:163676/140‑1 (MQ=255)

                                                 tCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCt                                                                          >  2:259799/1‑140 (MQ=255)

                                                    gCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAg                                                                       <  2:216350/140‑1 (MQ=255)

                                                     cGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGt                                                                      >  2:533535/1‑140 (MQ=255)

                                                         aGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAg                                                                  <  1:206360/140‑1 (MQ=255)

                                                           aaTCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAgcg                                                                <  2:226195/140‑1 (MQ=255)

                                                           aaTCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAgcg                                                                <  1:820434/140‑1 (MQ=255)

                                                             tCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCa                                                              >  2:66079/1‑140 (MQ=255)

                                                                 cTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCtt                                                          <  2:534404/140‑1 (MQ=255)

                                                                       cTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTAcggc                                                    <  1:66079/140‑1 (MQ=255)

                                                                         tGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTAcggcgg                                                  >  1:475008/1‑140 (MQ=255)

                                                                                                     gcccgcGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCtt                      <  2:127817/140‑1 (MQ=255)

                                                                                                      cccgcGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCAGTTTTCCCgcgccgcgcgccgcccc                     >  2:546530/1‑137 (MQ=255)

                                                                                                       ccgcGGAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTca                    <  2:400024/140‑1 (MQ=255)

                                                                                                            gAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgggc               <  2:558318/140‑1 (MQ=255)

                                                                                                            gAGTCCCCTTCCACCAGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTcacgggc               <  1:842063/140‑1 (MQ=255)

                                                                                                                         ccaGGTACAGTCCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGGCCTTTTTCGCGCGCGGCGCGCCCCCTCCCCGGGCCGGGGGGCATCg  >  2:46130/1‑140 (MQ=255)

GAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCACCAGGTACAGTTCGGAAAGCGCCGGATCGCGTTCCTGGCAGTCTGCCAGTTTGCCCGGCAGGCCCGCTAAGTCGAGCGCACCTTTACGGCGGGTCATTTCACGCGCGCGACGCGCCGCTTCACGGGCACGGGCAGCATCG  >  NC_000913/3878732‑3878992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A