Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F28 I1 R1
|
260 |
73.5 |
2514320 |
99.3% |
2496719 |
138.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,879,326 |
T→C |
N265S (AAC→AGC) |
gyrB ← |
DNA gyrase subunit B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,879,326 | 0 | T | C | 100.0%
| 208.4
/ NA
| 59 | N265S (AAC→AGC) | gyrB | DNA gyrase subunit B |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/30); total (29/30) |
GGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTGATGCCGCC > NC_000913/3879190‑3879462
|
ggCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGcttt > 2:420121/1‑140 (MQ=255)
gcgcTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTc > 1:580804/1‑140 (MQ=255)
gcTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCt > 2:944704/1‑140 (MQ=255)
cTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAg < 2:590834/140‑1 (MQ=255)
ggCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCAt < 2:523661/140‑1 (MQ=255)
ttttGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACTGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTc > 2:331812/1‑140 (MQ=255)
ttttGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTc < 1:713894/140‑1 (MQ=255)
ttGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCa > 1:278944/1‑140 (MQ=255)
gTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCa < 1:825195/140‑1 (MQ=255)
tAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCaa > 2:1256211/1‑140 (MQ=255)
ttcttTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCa > 2:114141/1‑140 (MQ=255)
cttTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACTGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACt < 1:331812/140‑1 (MQ=255)
gcccATGTAGGCTTTCAGGGTAGGGGTCATCGCCGCCCGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGa < 2:580804/138‑1 (MQ=255)
gTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAACCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGa < 2:1170972/140‑1 (MQ=255)
gTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCaa > 1:362806/1‑140 (MQ=255)
gTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCaa > 1:550023/1‑140 (MQ=255)
tAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACt < 1:422934/129‑1 (MQ=255)
tAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACt > 2:422934/1‑129 (MQ=255)
cGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCg > 1:99767/1‑140 (MQ=255)
ttCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTc > 1:772157/1‑140 (MQ=255)
tCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCt > 1:626691/1‑140 (MQ=255)
gggTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTg > 2:276449/1‑140 (MQ=255)
ggTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTgg < 1:1256211/140‑1 (MQ=255)
tCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGgag < 2:836368/140‑1 (MQ=255)
tCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGgag > 1:836368/1‑140 (MQ=255)
tCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGgag < 2:772157/140‑1 (MQ=255)
aTCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGaa > 2:435607/1‑140 (MQ=255)
aTCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGaa > 1:165342/1‑140 (MQ=255)
tCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAg < 2:340830/140‑1 (MQ=255)
tCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAg < 2:958146/140‑1 (MQ=255)
cgccgcACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGT‑AAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTa > 1:1021462/1‑140 (MQ=255)
cgcACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTaga < 2:22001/140‑1 (MQ=255)
cACGGAAGCCTGCCAGG‑GAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTagaaga > 1:1011696/1‑140 (MQ=255)
aCGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTagaaga < 2:99767/140‑1 (MQ=255)
ggAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGata < 1:944704/140‑1 (MQ=255)
cTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCggg < 1:276449/140‑1 (MQ=255)
ccgTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGtt > 1:226407/1‑135 (MQ=255)
ccgTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGtt < 2:226407/135‑1 (MQ=255)
ccgTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTttgtt < 1:767167/140‑1 (MQ=255)
cgTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTttgttc < 2:902510/140‑1 (MQ=255)
cGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTttgttcttgtt < 1:435607/140‑1 (MQ=255)
gCGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGa < 2:1011696/140‑1 (MQ=255)
cGGAATGTTGTTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGat < 2:626691/140‑1 (MQ=255)
tgttgtTGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCa > 2:726664/1‑140 (MQ=255)
gttgttGGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCaa > 2:1205728/1‑140 (MQ=255)
tgttgGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCaacg > 2:801867/1‑140 (MQ=255)
tgttgGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCaacg < 2:550023/140‑1 (MQ=255)
ttgGTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCaacgaa < 2:362806/140‑1 (MQ=255)
gTAAAGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGc > 1:869406/1‑140 (MQ=255)
aGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTg > 1:418220/1‑140 (MQ=255)
aGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTg > 2:1166124/1‑140 (MQ=255)
aGCAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTg > 1:225940/1‑140 (MQ=255)
cAGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTGAt < 2:869406/140‑1 (MQ=255)
aGTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTGATg > 2:1046651/1‑140 (MQ=255)
gTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTGATgc < 1:420121/140‑1 (MQ=255)
gTAGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTGATgc < 1:726664/140‑1 (MQ=255)
aGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTGATgccg > 1:908904/1‑140 (MQ=255)
aGATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTGATgccg > 1:765417/1‑140 (MQ=255)
gATGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTGATgccgc < 2:908904/140‑1 (MQ=255)
aTGCTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTGATgccgcc < 2:225940/140‑1 (MQ=255)
|
GGCGCTGACTTTGGCTTTTTTGCTGTAGCCTTCTTTGTCCATGTAGGCGTTCAGGGTACGGGTCATCGCCGCACGGAAGCCTGCCAGGTGAGTACCGCCGTCACGCTGCGGAATGTTGTTGGTAAAGCAGTAGATGTTTTCCTGGAAGCCATCGTTCCACTGCAACGCCACTTCGACGCCAATACCGTCTTTTTCAGTGGAGAAGTAGAAGATATTCGGGTGGATCGGCGTTTTGTTCTTGTTCAGATATTCAACGAACGCCTTGATGCCGCC > NC_000913/3879190‑3879462
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
