Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,079,819	T→C	R23R (CGT→CGC)	yiiF →	protein YiiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,079,819	0	T	C	97.4%	266.7 / ‑2.3	78	R23R (CGT→CGC)	yiiF	protein YiiF
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (36/40); total (37/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TCTCTCCATAGATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTG  >  NC_000913/4079688‑4079954

tctctcCATAGATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTACAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTcc                                                                                                                                 <  1:76589/140‑1 (MQ=255)

  tctcCATAGATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTc                                                                                                                               >  2:450521/1‑140 (MQ=255)

     ccATAGATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTg                                                                                                                            <  2:263907/140‑1 (MQ=255)

     ccATAGATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTg                                                                                                                            <  1:234268/140‑1 (MQ=255)

       aTAGATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCg                                                                                                                          >  2:226262/1‑140 (MQ=255)

       aTAGATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCACAATCTTCCTCGTGCg                                                                                                                          <  2:50075/140‑1 (MQ=255)

         aGATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGa                                                                                                                        <  1:442001/140‑1 (MQ=255)

          gATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGAc                                                                                                                       <  1:576476/140‑1 (MQ=255)

           aTTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGAcc                                                                                                                      >  2:52730/1‑140 (MQ=255)

            ttCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTAAAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCg                                                                                                                          >  1:452538/1‑135 (MQ=255)

            ttCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTAAAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCg                                                                                                                          <  2:452538/135‑1 (MQ=255)

             tCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTa                                                                                                                    <  1:226262/140‑1 (MQ=255)

                    tGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAggg                                                                                                             <  2:983011/140‑1 (MQ=255)

                     gATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGa                                                                                                            >  1:95830/1‑140 (MQ=255)

                     gATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGa                                                                                                            <  1:592545/140‑1 (MQ=255)

                     gATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGa                                                                                                            >  1:1046978/1‑140 (MQ=255)

                     gATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGa                                                                                                            <  1:759511/140‑1 (MQ=255)

                     gATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGa                                                                                                            <  1:212032/140‑1 (MQ=255)

                      atatTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTCACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGaa                                                                                                           >  2:240575/1‑140 (MQ=255)

                       tatTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAg                                                                                                          <  2:102617/140‑1 (MQ=255)

                           ggCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGt                                                                                                      <  2:564320/140‑1 (MQ=255)

                           ggCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGt                                                                                                      <  1:112453/140‑1 (MQ=255)

                               agaTATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGAt                                                                                                  <  1:450521/140‑1 (MQ=255)

                               agaTATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTCACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGAt                                                                                                  <  1:240575/140‑1 (MQ=255)

                                            ctctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATaa                                                                                     >  1:90627/1‑140 (MQ=255)

                                            ctctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATaa                                                                                     >  1:61626/1‑140 (MQ=255)

                                              ctctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAaat                                                                                   <  1:747647/140‑1 (MQ=255)

                                               tctGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAaatc                                                                                  >  2:745438/1‑140 (MQ=255)

                                                         ataGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAAc                                                                        >  1:639131/1‑140 (MQ=255)

                                                                 gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCa                                                                    >  2:856888/1‑136 (MQ=255)

                                                                 gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCa                                                                    <  1:856888/136‑1 (MQ=255)

                                                                 gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGaa                                                                >  2:1073031/1‑140 (MQ=255)

                                                                 gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGaa                                                                >  2:196598/1‑140 (MQ=255)

                                                                 gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGaa                                                                <  2:61626/140‑1 (MQ=255)

                                                                 gggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGaa                                                                >  1:208914/1‑140 (MQ=255)

                                                                  ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGTACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                               >  2:991140/1‑140 (MQ=255)

                                                                  ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCaaa                                                                         <  1:877566/130‑1 (MQ=255)

                                                                  ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCaaa                                                                         >  2:877566/1‑130 (MQ=255)

                                                                  ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                               >  2:602999/1‑140 (MQ=255)

                                                                  ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                               >  1:309879/1‑140 (MQ=255)

                                                                  ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                               >  2:984696/1‑140 (MQ=255)

                                                                  ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                               >  1:508224/1‑140 (MQ=255)

                                                                  ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                               >  1:580574/1‑140 (MQ=255)

                                                                  ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                               <  2:208914/140‑1 (MQ=255)

                                                                  ggCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAAc                                                               >  2:1073776/1‑140 (MQ=255)

                                                                   gCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCaaa                                                                         >  2:885579/1‑129 (MQ=255)

                                                                   gCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCaaa                                                                         <  1:885579/129‑1 (MQ=255)

                                                                     aGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAg                                                            <  2:86033/140‑1 (MQ=255)

                                                                      gAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAgt                                                           <  1:151647/140‑1 (MQ=255)

                                                                            tACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCt                                                     <  2:95830/140‑1 (MQ=255)

                                                                            tACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCt                                                     >  1:120474/1‑140 (MQ=255)

                                                                            tACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCt                                                     >  1:262360/1‑140 (MQ=255)

                                                                              cTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTgg                                                   <  1:745438/140‑1 (MQ=255)

                                                                                cGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCa                                                 <  2:262360/140‑1 (MQ=255)

                                                                                 gATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCAt                                                >  1:664613/1‑140 (MQ=255)

                                                                                   tttATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCt                                              >  2:971993/1‑140 (MQ=255)

                                                                                        cGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                         <  1:602999/140‑1 (MQ=255)

                                                                                        cGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                         <  1:991140/140‑1 (MQ=255)

                                                                                        cGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                         <  1:1073776/140‑1 (MQ=255)

                                                                                        cGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCaa                                         <  1:454186/140‑1 (MQ=255)

                                                                                            tGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGgt                                     <  1:1073031/140‑1 (MQ=255)

                                                                                              aGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGgtgt                                   <  1:196598/140‑1 (MQ=255)

                                                                                              aGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGgtgt                                   <  2:309879/140‑1 (MQ=255)

                                                                                                   aTTAAGCAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGa                              >  2:581177/1‑140 (MQ=255)

                                                                                                         cAACTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATgg                        <  1:581177/140‑1 (MQ=255)

                                                                                                            cTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGgtgt                     >  1:97014/1‑140 (MQ=255)

                                                                                                            cTTGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGgtgt                     >  2:556122/1‑140 (MQ=255)

                                                                                                             ttGATGATCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTc                    >  1:584840/1‑140 (MQ=255)

                                                                                                                 tgatCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAat                >  2:566470/1‑140 (MQ=255)

                                                                                                                 tgatCTCGAGGAGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAat                >  2:600415/1‑140 (MQ=255)

                                                                                                                  gatCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAata               <  1:971993/140‑1 (MQ=255)

                                                                                                                   atCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAatat              <  2:120474/140‑1 (MQ=255)

                                                                                                                   atCTCGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAatat              <  1:984696/140‑1 (MQ=255)

                                                                                                                      tcGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAg           <  2:508224/140‑1 (MQ=255)

                                                                                                                       cGAGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGc          >  1:22685/1‑140 (MQ=255)

                                                                                                                         aGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAata               >  2:380811/1‑133 (MQ=255)

                                                                                                                         aGGTGCAGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAata               <  1:380811/133‑1 (MQ=255)

                                                                                                                               aGCGCAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTg  >  1:949699/1‑140 (MQ=255)

TCTCTCCATAGATTCAAAAATGATATTGGCGAGATATTTATGAACTCTCTGGCAGGTATAGATATGGGCAGAATTTTACTCGATTTATCGAATGAGGTGATTAAGCAACTTGATGATCTCGAGGTGCAGCGTAATCTTCCTCGTGCGGACCTATTAAGGGAAGCGGTAGATCAATACCTGATAAATCAATCGCAAACAGCAAGAACCAGTGTTCCTGGCATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTG  >  NC_000913/4079688‑4079954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A