Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,232,017	A→G	V406A (GTA→GCA)	lysC ←	aspartate kinase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,232,017	0	A	G	100.0%	329.6 / NA	93	V406A (GTA→GCA)	lysC	aspartate kinase III
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (61/32); total (61/32)

TATTTACTCAAACAAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGT  >  NC_000913/4231881‑4232153

tATTTACTCAAACAAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCct                                                                                                                                       >  2:276808/1‑140 (MQ=255)

 aTTTACTCAAACAAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCctc                                                                                                                                      >  2:768009/1‑140 (MQ=255)

  tttACTCAAACAAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCctct                                                                                                                                     >  1:732706/1‑140 (MQ=255)

  tttACTCAAACAAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCctct                                                                                                                                     >  2:1027354/1‑140 (MQ=255)

   ttACTCAAACAAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCtt                                                                                                                                    >  2:601452/1‑140 (MQ=255)

   ttACTCAAACAAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCtt                                                                                                                                    >  1:733910/1‑140 (MQ=255)

     aCTCAAACAAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTg                                                                                                                                  >  1:726194/1‑140 (MQ=255)

     aCTCAAACAAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGCTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTg                                                                                                                                  >  2:84620/1‑140 (MQ=255)

        caaacaaaTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCa                                                                                                                               >  2:284405/1‑140 (MQ=255)

          aacaaaTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAAc                                                                                                                             >  1:341142/1‑140 (MQ=255)

          aacaaaTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAAc                                                                                                                             >  1:401319/1‑140 (MQ=255)

          aacaaaTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAAc                                                                                                                             >  1:268826/1‑140 (MQ=255)

          aacaaaTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAAc                                                                                                                             >  2:353100/1‑140 (MQ=255)

          aacaaaTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAAc                                                                                                                             >  1:1003526/1‑140 (MQ=255)

          aacaaaTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAAc                                                                                                                             >  1:1006305/1‑140 (MQ=255)

           acaaaTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAAcg                                                                                                                            >  2:863528/1‑140 (MQ=255)

               aTTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAAcgccgc                                                                                                                        <  1:768009/140‑1 (MQ=255)

                  aCTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAgg                                                                                                                     <  2:848894/140‑1 (MQ=255)

                       gCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCtttt                                                                                                                >  2:444738/1‑140 (MQ=255)

                           tttttGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACa                                                                                                            >  1:119769/1‑140 (MQ=255)

                              ttGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGt                                                                                                         <  2:119769/140‑1 (MQ=255)

                              ttGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGt                                                                                                         <  1:276808/140‑1 (MQ=255)

                                gCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCa                                                                                                       >  2:948380/1‑140 (MQ=255)

                                     accTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGcc                                                                                                  >  1:724978/1‑140 (MQ=255)

                                       cTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCaa                                                                                                <  1:521256/140‑1 (MQ=255)

                                              gCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAAcgc                                                                                         >  1:332335/1‑140 (MQ=255)

                                                aTCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCACCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGa                                                                                       >  1:798021/1‑140 (MQ=255)

                                                 tCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGCACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGAc                                                                                      >  1:403131/1‑140 (MQ=255)

                                                  cTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGAcc                                                                                     >  2:707644/1‑140 (MQ=255)

                                                  cTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGAcc                                                                                     <  2:332335/140‑1 (MQ=255)

                                                         gggCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCa                                                                              <  1:1109666/140‑1 (MQ=255)

                                                           gCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGa                                                                            <  2:732706/140‑1 (MQ=255)

                                                           gCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGa                                                                            <  2:726194/140‑1 (MQ=255)

                                                            cACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGCACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGAc                                                                           <  2:403131/140‑1 (MQ=255)

                                                                      cacaGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGGACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTtccac                                                                 >  2:774924/1‑140 (MQ=255)

                                                                       acaGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTtccacc                                                                <  1:838981/140‑1 (MQ=255)

                                                                        caGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTtccacct                                                               <  2:724978/140‑1 (MQ=255)

                                                                        caGGTTATGCGTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTCCGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTtccacct                                                               <  2:747992/140‑1 (MQ=255)

                                                                         aGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTtccacctc                                                              <  2:858894/140‑1 (MQ=255)

                                                                         aGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTtccacctc                                                              >  1:906438/1‑140 (MQ=255)

                                                                          ggTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTtccacctcc                                                             <  1:601452/140‑1 (MQ=255)

                                                                           gTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCGtccacctcca                                                            >  1:1055398/1‑140 (MQ=255)

                                                                                ggCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGa                                                       <  2:906438/140‑1 (MQ=255)

                                                                                ggCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACACCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGa                                                       >  2:337462/1‑140 (MQ=255)

                                                                                  cTGGATGCGCCATAACACATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGaca                                                     <  2:1006305/140‑1 (MQ=255)

                                                                                  cTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCTGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGaca                                                     <  2:1055398/140‑1 (MQ=255)

                                                                                  cTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGaca                                                     <  2:13092/140‑1 (MQ=255)

                                                                                  cTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGaca                                                     <  2:401319/140‑1 (MQ=255)

                                                                                  cTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGaca                                                     <  1:707644/140‑1 (MQ=255)

                                                                                    ggATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGaca                                                     >  1:1035071/1‑138 (MQ=255)

                                                                                    ggATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGaca                                                     <  2:1035071/138‑1 (MQ=255)

                                                                                    ggATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGAcaca                                                   >  2:428917/1‑140 (MQ=255)

                                                                                     gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAg                                                  >  2:815673/1‑140 (MQ=255)

                                                                                     gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAg                                                  >  1:50977/1‑140 (MQ=255)

                                                                                     gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAg                                                  >  1:182518/1‑140 (MQ=255)

                                                                                     gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAg                                                  >  2:299977/1‑140 (MQ=255)

                                                                                     gATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTACCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAg                                                  >  1:1077071/1‑140 (MQ=255)

                                                                                      aTGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGt                                                 >  1:493254/1‑140 (MQ=255)

                                                                                      aTGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGt                                                 >  1:466998/1‑140 (MQ=255)

                                                                                      aTGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGACATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGt                                                 >  2:1082043/1‑140 (MQ=255)

                                                                                          gcCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCgg                                             <  1:428917/140‑1 (MQ=255)

                                                                                                   aaTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCa                                    <  1:904342/140‑1 (MQ=255)

                                                                                                    aTCATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCAt                                   >  1:298948/1‑140 (MQ=255)

                                                                                                      cATGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATTAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATca                                 >  2:387144/1‑140 (MQ=255)

                                                                                                        tGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagc                               >  2:644667/1‑140 (MQ=255)

                                                                                                        tGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagc                               >  2:214160/1‑140 (MQ=255)

                                                                                                        tGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagc                               >  2:770181/1‑140 (MQ=255)

                                                                                                        tGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagc                               >  1:273155/1‑140 (MQ=255)

                                                                                                        tGCGAATGTTGAACGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagc                               >  1:33301/1‑140 (MQ=255)

                                                                                                                   aaCGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATcagca                              >  2:170647/1‑130 (MQ=255)

                                                                                                                   aaCGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGt                    >  1:1022806/1‑140 (MQ=255)

                                                                                                                   aaCGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCCCCACCCGACACAGTGCGGAAAGCTCCATcagca                              <  1:170647/130‑1 (MQ=255)

                                                                                                                     cGGTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCa                  <  1:770181/140‑1 (MQ=255)

                                                                                                                       gTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGc                <  1:1027354/140‑1 (MQ=255)

                                                                                                                       gTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGc                <  1:444738/140‑1 (MQ=255)

                                                                                                                       gTTCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGc                <  1:353100/140‑1 (MQ=255)

                                                                                                                        ttCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGAt                         >  1:337624/1‑130 (MQ=255)

                                                                                                                        ttCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGAt                         <  2:337624/130‑1 (MQ=255)

                                                                                                                         tCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCaa              >  1:1084789/1‑140 (MQ=255)

                                                                                                                         tCCAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCaa              >  1:254211/1‑140 (MQ=255)

                                                                                                                          ccAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAAc             >  1:117759/1‑140 (MQ=255)

                                                                                                                          ccAGTACGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCCCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAAc             >  1:209409/1‑140 (MQ=255)

                                                                                                                                cGCCGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCg       >  1:975731/1‑140 (MQ=255)

                                                                                                                                  ccGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGATCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGcc     >  2:1061557/1‑140 (MQ=255)

                                                                                                                                  ccGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGt           >  1:15480/1‑134 (MQ=255)

                                                                                                                                  ccGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGt           <  2:15480/134‑1 (MQ=255)

                                                                                                                                  ccGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGcc     >  2:273939/1‑140 (MQ=255)

                                                                                                                                  ccGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGcc     >  2:840359/1‑140 (MQ=255)

                                                                                                                                  ccGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGcc     >  1:936804/1‑140 (MQ=255)

                                                                                                                                  ccGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGcc     >  1:201902/1‑140 (MQ=255)

                                                                                                                                  ccGAATGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGcc     >  2:7020/1‑140 (MQ=255)

                                                                                                                                     aaTGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGc                >  2:174492/1‑126 (MQ=255)

                                                                                                                                     aaTGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGc                <  1:174492/126‑1 (MQ=255)

                                                                                                                                     aaTGCCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGt  <  2:117759/140‑1 (MQ=255)

TATTTACTCAAACAAATTACTATGCAGTTTTTGCACCACCTGCTCGGCATCTTCGCCGGGCACCAGGAAGCACAGGTTATGGCTGGATGCGCCATAACAAATCATGCGAATGTTGAACGGTTCCAGTACGCCGAATACCTCTTTGCCAACGCCGCAGGCTTTTGACAGGTCATTGCCAATCAACGCGACCAGCGCCAGACCTTCTTCCACCTCCACCCGACACAGTGCGGAAAGCTCCATCAGCAGAGATTGCGTCAGCAACGTATCGCCAGT  >  NC_000913/4231881‑4232153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A