Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,331,708	A→G	T403A (ACG→GCG)	proP →	osmolyte:H(+) symporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,331,708	0	A	G	100.0%	168.2 / NA	48	T403A (ACG→GCG)	proP	osmolyte:H(+) symporter ProP
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/31); total (17/31)

GCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGA  >  NC_000913/4331585‑4331822

gCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTggcggc                                                                                                    >  1:501378/1‑140 (MQ=255)

    tGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCctgg                                                                                                <  1:341805/140‑1 (MQ=255)

      aTCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCctggct                                                                                              >  2:965936/1‑140 (MQ=255)

           tAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCg                                                                                         <  2:572958/140‑1 (MQ=255)

              cTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGaaa                                                                                      >  2:952207/1‑140 (MQ=255)

                 cTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGTGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                                                                                   >  1:1013020/1‑140 (MQ=255)

                 cTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                                                                                   >  2:799351/1‑140 (MQ=255)

                 cTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                                                                                   >  2:751239/1‑140 (MQ=255)

                 cTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                                                                                   >  2:497711/1‑140 (MQ=255)

                  tttACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTc                                                                                  >  2:609395/1‑140 (MQ=255)

                  tttACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTc                                                                                  >  2:247191/1‑140 (MQ=255)

                   ttACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCg                                                                                 >  1:1064545/1‑140 (MQ=255)

                   ttACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCg                                                                                 <  2:1026283/140‑1 (MQ=255)

                    tACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGc                                                                                <  1:932872/140‑1 (MQ=255)

                    tACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGc                                                                                >  2:932872/1‑140 (MQ=255)

                     aCGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCa                                                                               <  2:1119499/140‑1 (MQ=255)

                     aCGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCa                                                                               <  1:482003/140‑1 (MQ=255)

                             tATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCtga                                                                       <  2:583480/140‑1 (MQ=255)

                              aTGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCtgat                                                                      <  1:218116/140‑1 (MQ=255)

                                   ttCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgc                                                                 <  1:751239/140‑1 (MQ=255)

                                   ttCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgc                                                                 <  1:336366/140‑1 (MQ=255)

                                    tCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc                                                                <  1:609395/140‑1 (MQ=255)

                                    tCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc                                                                <  1:468081/140‑1 (MQ=255)

                                    tCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc                                                                <  1:965936/140‑1 (MQ=255)

                                     cTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGCTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcct                                                               <  2:994431/140‑1 (MQ=255)

                                      tACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcctg                                                              <  2:300706/140‑1 (MQ=255)

                                       aCCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcctgc                                                             >  1:1010869/1‑140 (MQ=255)

                                          ttGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTa                                                          <  1:488504/140‑1 (MQ=255)

                                           tGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt                                                         >  1:123792/1‑140 (MQ=255)

                                            gCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCtgat                                                                      >  1:846242/1‑126 (MQ=255)

                                            gCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCtgat                                                                      <  2:846242/126‑1 (MQ=255)

                                            gCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc                                                                <  2:129728/132‑1 (MQ=255)

                                            gCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc                                                                >  1:129728/1‑132 (MQ=255)

                                             ccAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTa                                                       <  1:155803/140‑1 (MQ=255)

                                              cAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTAc                                                      <  2:501378/140‑1 (MQ=255)

                                                gCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc                                                                <  2:211798/128‑1 (MQ=255)

                                                gCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc                                                                >  1:211798/1‑128 (MQ=255)

                                                   aTGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGAt                                                 <  1:497711/140‑1 (MQ=255)

                                                       tCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTa                                             <  1:247191/140‑1 (MQ=255)

                                                           gACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTgg                                         <  2:611871/140‑1 (MQ=255)

                                                                            gcgcgcTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTgg                                         >  2:553633/1‑123 (MQ=255)

                                                                            gcgcgcTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTgg                                         <  1:553633/123‑1 (MQ=255)

                                                                            gcgcgcTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATc                        <  2:556452/140‑1 (MQ=255)

                                                                              gcgcTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGTGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCAc                      <  2:1013020/140‑1 (MQ=255)

                                                                              gcgcTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCAc                      <  1:888837/140‑1 (MQ=255)

                                                                               cgcTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGGTTTATTAcc                     >  1:470960/1‑140 (MQ=255)

                                                                                    gcggcggcATTTAATATTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGt                <  1:508454/140‑1 (MQ=255)

                                                                                        cggcATTTAATATTTCGGTGCTAGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAAcc            <  1:799351/140‑1 (MQ=255)

                                                                                                  tatTTCGGTGCTGGTTGCCGGTCTGGCGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAgaga  <  2:1117756/140‑1 (MQ=255)

GCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGA  >  NC_000913/4331585‑4331822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A