Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	180	66.5	2263572	99.4%	2249990	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,414,863	A→G	T197A (ACC→GCC)	aidB →	putative acyl‑CoA dehydrogenase AidB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,414,863	0	A	G	100.0%	173.2 / NA	49	T197A (ACC→GCC)	aidB	putative acyl‑CoA dehydrogenase AidB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/28); total (21/28)

CGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCTTTTTTGTGCCG  >  NC_000913/4414726‑4414997

cgcCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAAcgcc                                                                                                                                      >  1:741986/1‑140 (MQ=255)

 gcCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAAcgcca                                                                                                                                     <  1:564081/140‑1 (MQ=255)

  ccGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAAcgccac                                                                                                                                    <  2:669766/140‑1 (MQ=255)

  ccGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAAcgccac                                                                                                                                    >  1:495897/1‑140 (MQ=255)

         aGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCa                                                                                                                             <  1:340331/140‑1 (MQ=255)

         aGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCa                                                                                                                             <  2:495897/140‑1 (MQ=255)

           gACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCaga                                                                                                                           <  1:294953/140‑1 (MQ=255)

             cgggACCACGCCGCGATGGAGCGATGGCGGCGATTCTCACCTATTGCGAGGTGGGCAAGAACGCGGTTTGTTGATTGGCATGGGCATGCGGGAAAAGCAGCGCGGTTCCGCTGTGATGAGCAACGCCCGCCGTGCAGAGc                                                                                                                         <  2:1023720/138‑1 (MQ=255)

               ggACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGt                                                                                                                       <  2:95184/140‑1 (MQ=255)

                gACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTc                                                                                                                      >  1:198433/1‑140 (MQ=255)

                gACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTc                                                                                                                      >  1:838155/1‑140 (MQ=255)

                gACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTc                                                                                                                      >  1:11965/1‑140 (MQ=255)

                     cgccgcTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGaa                                                                                                                 >  2:636311/1‑140 (MQ=255)

                     cgccgcTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGaa                                                                                                                 >  2:174158/1‑140 (MQ=255)

                       ccgcTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGa                                                                                                               <  1:174158/140‑1 (MQ=255)

                            aTTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGct                                                                                                          <  1:892568/140‑1 (MQ=255)

                                aGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATgg                                                                                                            >  1:1067561/1‑134 (MQ=255)

                                aGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATgg                                                                                                            <  2:1067561/134‑1 (MQ=255)

                                 gCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTAt                                                                                                     <  2:225319/140‑1 (MQ=255)

                                     tCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGc                                                                                                 <  1:121519/140‑1 (MQ=255)

                                     tCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGc                                                                                                 <  2:198433/140‑1 (MQ=255)

                                                   aCTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATaaa                                                                                   >  2:329583/1‑140 (MQ=255)

                                                      tATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATgg                                                                                <  1:125829/140‑1 (MQ=255)

                                                        ttGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGtt                                                                              >  2:779757/1‑140 (MQ=255)

                                                          gCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGtttt                                                                            >  1:429471/1‑140 (MQ=255)

                                                               gTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCg                                                                       >  2:643806/1‑140 (MQ=255)

                                                                tGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCgg                                                                      <  2:741986/140‑1 (MQ=255)

                                                                 gggCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGt                                                                     <  1:329583/140‑1 (MQ=255)

                                                                       aaaCGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCa                                                               <  2:11965/140‑1 (MQ=255)

                                                                        aaCGCGGTTTGTTGATTGGCACGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCaa                                                              <  2:1030984/140‑1 (MQ=255)

                                                                                 tgttgATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATgcg                                                     >  1:218494/1‑140 (MQ=255)

                                                                                  gttgATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATgcgc                                                    <  1:643806/140‑1 (MQ=255)

                                                                                         ggCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCt                                                >  2:105350/1‑137 (MQ=255)

                                                                                         ggCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCt                                                <  1:105350/137‑1 (MQ=255)

                                                                                          gCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTg                                            <  2:429471/140‑1 (MQ=255)

                                                                                           cATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCCAAGCGATGCGCATCTGGGGc                                           >  2:671436/1‑140 (MQ=255)

                                                                                             tGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTg                                            <  2:1120495/137‑1 (MQ=255)

                                                                                             tGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTg                                            >  1:1120495/1‑137 (MQ=255)

                                                                                                   tGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAg                                   <  2:895437/140‑1 (MQ=255)

                                                                                                    gACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGa                                  >  2:986801/1‑140 (MQ=255)

                                                                                                     aCGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGAc                                 <  2:838155/140‑1 (MQ=255)

                                                                                                          aaaGCAGGGCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCgg                            <  2:218494/140‑1 (MQ=255)

                                                                                                                 ggCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAg                                   >  1:723059/1‑126 (MQ=255)

                                                                                                                 ggCGGTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAg                                   <  2:723059/126‑1 (MQ=255)

                                                                                                                    ggTTCCGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTc                  <  2:733761/140‑1 (MQ=255)

                                                                                                                        ccGATGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGc              >  2:599472/1‑140 (MQ=255)

                                                                                                                           aTGTTATGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGGGGGGCATAAATGGGTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCttt           >  2:608395/1‑140 (MQ=255)

                                                                                                                                aTGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGTATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCTTTTTtgt      >  1:478102/1‑140 (MQ=255)

                                                                                                                                aTGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCTTTTTtgt      >  2:869755/1‑140 (MQ=255)

                                                                                                                                aTGAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGGGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCTTTTTtgt      >  2:268952/1‑140 (MQ=255)

                                                                                                                                  gAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCTTTTTTGTGc    <  1:779757/140‑1 (MQ=255)

                                                                                                                                  gAGCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCTTTTTTGTGc    <  1:400400/140‑1 (MQ=255)

                                                                                                                                    gCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCTTTTTTGTGCcg  <  1:636311/140‑1 (MQ=255)

                                                                                                                                    gCAACGCCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCTTTTTTGTGCcg  <  1:986801/140‑1 (MQ=255)

CGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGCTCTTATCGGCTGGTGGGGCATAAATGGTTTTTCTCGGTTCCGCAAAGCGATGCGCATCTGGTGCTGGCGCAGACCGCGGGTGGTCTGTCCTGCTTTTTTGTGCCG  >  NC_000913/4414726‑4414997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A