Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	48,757	T→C	V330A (GTG→GCG)	kefC →	K(+) : H(+) antiporter KefC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	48,757	0	T	C	97.9%	162.6 / ‑4.5	48	V330A (GTG→GCG)	kefC	K(+) : H(+) antiporter KefC
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (23/24); total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGATTCTGC  >  NC_000913/48623‑48891

cTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGcgttgt                                                                                                                                   <  1:1074249/140‑1 (MQ=255)

cTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGcgttgt                                                                                                                                   <  2:559428/140‑1 (MQ=255)

    ggCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTagg                                                                                                                               <  2:780636/140‑1 (MQ=255)

      cACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTagggc                                                                                                                             <  2:33100/140‑1 (MQ=255)

      cACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTagggt                                                                                                                             <  2:886263/140‑2 (MQ=255)

      cACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTagggc                                                                                                                             <  2:625027/140‑1 (MQ=255)

           tgctTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTagggcagggc                                                                                                                        >  2:1137622/1‑140 (MQ=255)

                     cccATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTagggcag                                                                                                                           <  2:845953/127‑1 (MQ=255)

                     cccATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTagggcag                                                                                                                           >  1:845953/1‑127 (MQ=255)

                     cccATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTg                                                                                                              >  2:1010261/1‑140 (MQ=255)

                      ccATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGc                                                                                                             <  1:935714/140‑1 (MQ=255)

                        aTTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCt                                                                                                           <  2:670399/140‑1 (MQ=255)

                          tGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAgggcagggca                                                                                                                       <  2:393154/126‑1 (MQ=255)

                          tGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAgggcagggca                                                                                                                       >  1:393154/1‑126 (MQ=255)

                             gcATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTtgtg                                                                                                      <  1:820045/140‑1 (MQ=255)

                             gcATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTtgtg                                                                                                      <  2:424091/140‑1 (MQ=255)

                               aTTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGt                                                                                                    >  1:107478/1‑140 (MQ=255)

                                ttGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTa                                                                                                   >  2:730064/1‑140 (MQ=255)

                                      ttttGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTggc                                                                                             >  2:559347/1‑140 (MQ=255)

                                       tttGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTggcg                                                                                            <  2:393411/140‑1 (MQ=255)

                                        ttGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTggcgc                                                                                           <  1:136688/140‑1 (MQ=255)

                                             gctCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTggcgcggcgc                                                                                      >  1:149215/1‑140 (MQ=255)

                                              ctCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCa                                                                                     >  2:849302/1‑140 (MQ=255)

                                                      ccTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGa                                                                             <  2:243352/140‑1 (MQ=255)

                                                        tcatcatcaAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAAt                                                                           >  2:681260/1‑140 (MQ=255)

                                                                    tCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCg                                                               >  2:806713/1‑140 (MQ=255)

                                                                    tCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCg                                                               <  1:559347/140‑1 (MQ=255)

                                                                                     aTTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCt                                              <  2:107478/140‑1 (MQ=255)

                                                                                     aTTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCt                                              <  2:412792/140‑1 (MQ=255)

                                                                                           cGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCt                                        >  1:1068966/1‑140 (MQ=255)

                                                                                           cGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCt                                        >  1:405225/1‑140 (MQ=255)

                                                                                                 ttGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGt                                  >  1:848961/1‑140 (MQ=255)

                                                                                                   gCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTgg                                >  1:417056/1‑140 (MQ=255)

                                                                                                   gCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTgg                                >  1:905740/1‑140 (MQ=255)

                                                                                                    cAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGgc                               >  2:568971/1‑140 (MQ=255)

                                                                                                        tGCCAAATAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTg                           >  2:673007/1‑140 (MQ=255)

                                                                                                             aaTAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGAt                      <  1:701205/140‑1 (MQ=255)

                                                                                                             aaTAAACAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGAt                      <  2:544762/140‑1 (MQ=255)

                                                                                                                   cAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGgcagc                <  2:149215/140‑1 (MQ=255)

                                                                                                                   cAGCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGgcagc                <  1:849302/140‑1 (MQ=255)

                                                                                                                     gCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCaa              >  2:706232/1‑140 (MQ=255)

                                                                                                                     gCGTCGTTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCaa              >  2:829339/1‑140 (MQ=255)

                                                                                                                      cgtcgtTGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCAAc             >  1:248514/1‑140 (MQ=255)

                                                                                                                         cgtTGGTTTGCGGCGTTGTTAGGACAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCaa              >  2:1190316/1‑136 (MQ=255)

                                                                                                                         cgtTGGTTTGCGGCGTTGTTAGGACAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCaa              <  1:1190316/136‑1 (MQ=255)

                                                                                                                           ttGGTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGa        <  2:405225/140‑1 (MQ=255)

                                                                                                                             ggTTTGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGAtt      <  1:267323/140‑1 (MQ=255)

                                                                                                                                ttGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGATTctg   <  2:848961/140‑1 (MQ=255)

                                                                                                                                ttGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGATTctg   >  2:934750/1‑140 (MQ=255)

                                                                                                                                 tGCGGCGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGATTctgc  <  1:1137622/140‑1 (MQ=255)

CTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTGCTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTGCCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTTGTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTGACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGATTCTGC  >  NC_000913/48623‑48891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A