Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,517,665 A→G E6E (GAA→GAG)  ydcY → DUF2526 domain‑containing protein YdcY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,517,6650AG100.0% 214.8 / NA 60E6E (GAA→GAG) ydcYDUF2526 domain‑containing protein YdcY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/33);  total (27/33)

TCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGA  >  NC_000913/1517531‑1517801
                                                                                                                                      |                                                                                                                                        
tCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCAt                                                                                                                                     <  1:680251/140‑1 (MQ=255)
  ccGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATcg                                                                                                                                   <  2:425260/140‑1 (MQ=255)
   cGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATcgc                                                                                                                                  >  2:857276/1‑140 (MQ=255)
              ttgttTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATg                                                                                                                       >  2:1068950/1‑140 (MQ=255)
               tgttTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGc                                                                                                                      <  1:452641/140‑1 (MQ=255)
                 ttttCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCcg                                                                                                                    <  1:857276/140‑1 (MQ=255)
                 ttttCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCcg                                                                                                                    <  2:151824/140‑1 (MQ=255)
                 ttttCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCcg                                                                                                                    <  1:492093/140‑1 (MQ=255)
                   ttCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACACAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTAGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCcgcg                                                                                                                  >  1:939795/1‑140 (MQ=255)
                        tGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGa                                                                                                             >  2:634961/1‑140 (MQ=255)
                        tGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGa                                                                                                             >  2:640968/1‑140 (MQ=255)
                           tttAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAaga                                                                                                          <  2:227213/140‑1 (MQ=255)
                              aaaTCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAagagag                                                                                                       <  1:486197/140‑1 (MQ=255)
                              aaaTCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAagagag                                                                                                       <  1:18662/140‑1 (MQ=255)
                                  cTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTc                                                                                                   <  1:238441/140‑1 (MQ=255)
                                    tACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAagaga                                                                                                        <  1:362407/133‑1 (MQ=255)
                                    tACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAagaga                                                                                                        >  2:362407/1‑133 (MQ=255)
                                    tACTCACTGGCTTGACGGCACCACGCAGACTTATATCATCTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCAt                                                                                                 >  1:600152/1‑140 (MQ=255)
                                            ggCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCata                                                                                         <  2:960424/140‑1 (MQ=255)
                                             gCTTGACGGCACCACGCAGCCTTATATCATTTGGATGAATCGATAACTTTCACAAGCGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGGCATCGCGCGCGCGGATGCCGCGATTGAAGAGAGCGTCATTGCCCctat                                                                                        >  2:1023645/1‑140 (MQ=255)
                                             gCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCa                                                                                                  <  2:683810/130‑1 (MQ=255)
                                             gCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCa                                                                                                  >  1:683810/1‑130 (MQ=255)
                                                 gACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAAc                                                                                    <  1:634961/140‑1 (MQ=255)
                                                    ggCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGaa                                                                                 <  2:1025213/140‑1 (MQ=255)
                                                      caccacGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAatt                                                                               <  1:421980/140‑1 (MQ=255)
                                                       accacGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAatta                                                                              >  1:97789/1‑140 (MQ=255)
                                                           cGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTaa                                                                          >  2:536320/1‑140 (MQ=255)
                                                           cGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTaa                                                                          >  1:715918/1‑140 (MQ=255)
                                                           cGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTaa                                                                          >  2:259666/1‑140 (MQ=255)
                                                           cGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTaa                                                                          >  2:35447/1‑140 (MQ=255)
                                                                          tttGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATg                                                           >  1:1059553/1‑140 (MQ=255)
                                                                          tttGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATg                                                           >  1:591365/1‑140 (MQ=255)
                                                                          tttGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATg                                                           >  1:997134/1‑140 (MQ=255)
                                                                          tttGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATg                                                           >  1:474997/1‑140 (MQ=255)
                                                                          tttGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATg                                                           >  1:1177982/1‑140 (MQ=255)
                                                                          tttGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATg                                                           >  1:1089415/1‑140 (MQ=255)
                                                                          tttGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCAATg                                                           >  2:774214/1‑140 (MQ=255)
                                                                             ggATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGAcg                                                        <  2:1089415/140‑1 (MQ=255)
                                                                               aTGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGAcgcg                                                      <  2:201579/140‑1 (MQ=255)
                                                                                   aTCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGt                                                  >  1:1172272/1‑140 (MQ=255)
                                                                                             ttCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGa                                        >  2:846982/1‑140 (MQ=255)
                                                                                              tCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCgggga                                       <  2:106122/140‑3 (MQ=255)
                                                                                              tCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGaa                                       <  2:1172272/140‑1 (MQ=255)
                                                                                              tCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGaa                                       <  2:715918/140‑1 (MQ=255)
                                                                                              tCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGaa                                       <  1:536320/140‑1 (MQ=255)
                                                                                              tCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCAATGACGCGGAGTTAAGTCGGGaa                                       <  1:774214/140‑1 (MQ=255)
                                                                                               cacaAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAg                                      <  1:1068950/140‑1 (MQ=255)
                                                                                                acaAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGa                                     <  2:591365/140‑1 (MQ=255)
                                                                                                acaAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGa                                     <  1:554882/140‑1 (MQ=255)
                                                                                                       gCTAAGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTac                              >  1:633586/1‑140 (MQ=255)
                                                                                                           aGGAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACAccc                          <  1:35447/140‑1 (MQ=255)
                                                                                                            ggAGAAAGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCca                         <  2:633586/140‑1 (MQ=255)
                                                                                                                aaaGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCcagcag                     >  1:433917/1‑140 (MQ=255)
                                                                                                                 aaGTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCCTATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCagcagc                    <  2:872698/140‑1 (MQ=255)
                                                                                                                   gTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCaa                  <  2:1059553/140‑1 (MQ=255)
                                                                                                                   gTATGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCaa                  <  2:997134/140‑1 (MQ=255)
                                                                                                                      tGTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGt               >  2:977973/1‑140 (MQ=255)
                                                                                                                       gTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCagcagc                    >  2:1221381/1‑134 (MQ=255)
                                                                                                                       gTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCagcagc                    <  1:1221381/134‑1 (MQ=255)
                                                                                                                       gTCGCATCTGGATGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTc              >  2:125899/1‑140 (MQ=255)
                                                                                                                                   tGAGGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGa  <  1:125899/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
TCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTGGCTAAGGAGAAAGTATGTCGCATCTGGATGAAGTCATCGCGCGCGTGGATGCCGCGATTGAAGAGAGCGTCATTGCCCATATGAACGAATTATTAATCGCCCTGAGCGATGACGCGGAGTTAAGTCGGGAAGATCGCTACACCCAGCAGCAACGTCTGCGCACAGCGA  >  NC_000913/1517531‑1517801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A