Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,679,237 T→C Y271H (TAC→CAC)  ydgH → DUF1471 domain‑containing protein YdgH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,679,2370TC100.0% 154.8 / NA 45Y271H (TAC→CAC) ydgHDUF1471 domain‑containing protein YdgH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/24);  total (21/24)

CTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAA  >  NC_000913/1679107‑1679371
                                                                                                                                  |                                                                                                                                       
cttTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACa                                                                                                                                <  1:862896/140‑1 (MQ=255)
       aCCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGa                                                                                                                         <  2:639750/140‑1 (MQ=255)
       aCCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGa                                                                                                                         <  2:574014/140‑1 (MQ=255)
        cccAGTCATCAAAAGGCGGGCGTTACTCCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGaa                                                                                                                        <  1:879286/140‑1 (MQ=255)
           aGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGtc                                                                                                                     >  1:651352/1‑140 (MQ=255)
           aGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGtc                                                                                                                     >  2:842311/1‑140 (MQ=255)
           aGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGtc                                                                                                                     >  1:614658/1‑140 (MQ=255)
           aGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGtc                                                                                                                     <  1:320408/140‑1 (MQ=255)
            gTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGtct                                                                                                                    >  1:592226/1‑140 (MQ=255)
             tcatcaAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGtctc                                                                                                                   >  1:714320/1‑140 (MQ=255)
                  aaaaGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATc                                                                                                              <  2:856190/140‑1 (MQ=255)
                     aGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAgg                                                                                                           <  2:456392/140‑1 (MQ=255)
                       gcgggcgTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGtt                                                                                                         >  1:131267/1‑140 (MQ=255)
                       gcgggcgTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGtt                                                                                                         <  1:842311/140‑1 (MQ=255)
                         gggcgTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGc                                                                                                       >  2:963570/1‑140 (MQ=255)
                          ggcgTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCg                                                                                                      >  1:23471/1‑140 (MQ=255)
                                   ccGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGcc                                                                                             <  2:968847/140‑1 (MQ=255)
                                     gTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCcgc                                                                                           >  1:342413/1‑140 (MQ=255)
                                     gTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCcgc                                                                                           >  2:662837/1‑140 (MQ=255)
                                           cTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCgaagaa                                                                                     <  2:614658/140‑1 (MQ=255)
                                           cTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCgaagaa                                                                                     <  2:1240707/140‑1 (MQ=255)
                                           cTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCgaagaa                                                                                     <  1:963570/140‑1 (MQ=255)
                                           cTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCgaagaa                                                                                     <  1:250593/140‑1 (MQ=255)
                                           cTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCgaagaa                                                                                     <  1:478214/140‑1 (MQ=255)
                                              ccGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGaaa                                                                                    >  1:1255101/1‑138 (MQ=255)
                                              ccGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGaaa                                                                                    <  2:1255101/138‑1 (MQ=255)
                                                ggATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTg                                                                                <  1:662837/140‑1 (MQ=255)
                                                 gATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑c                                                                              <  1:623288/140‑1 (MQ=255)
                                                 gATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑c                                                                              <  2:351838/140‑1 (MQ=255)
                                                   tGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTa                                                                            <  2:423176/140‑1 (MQ=255)
                                                   tGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTa                                                                            <  2:342413/140‑1 (MQ=255)
                                                      cACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGt                                                                         >  1:1011000/1‑140 (MQ=255)
                                                      cACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGt                                                                         >  1:542701/1‑140 (MQ=255)
                                                      cACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGt                                                                         >  1:1006228/1‑140 (MQ=255)
                                                        cTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTAt                                                                       <  1:1227039/140‑1 (MQ=255)
                                                               cGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACcaca                                                                <  2:1238309/140‑1 (MQ=255)
                                                                        aaaCAAAGCGCCCGCAGCGATGATGGCCCCGTTCGACAGCATCAAATTCTCGGGCACCCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTCCCACATCCCCCGcc                                                       <  2:131267/139‑1 (MQ=255)
                                                                                    cGCAGCGAGGATGGCCCCGTTCGACAGCATAAAATTCTCTGGCACCCACGCCAACATGACCGAAGTCTCCTATCGGGTTGCGAAACGTGCCGCGAAAAAAGGTG‑TAAAGTTTTACCACATCCCCCGCCAGTGGCAGGAAc                                           <  2:1027383/140‑1 (MQ=255)
                                                                                     gCAGCTTTGATGGTCCCGTTCCAAAATATAAAATTCTCGGCCACCCACGCAAACATGACCAATGTCTCTTACGTGTTTGCGAATCGTGCCGAAAGAAAAGGTG‑ATAAGTTTTCCAACATCCCCCGCCAGTGGCAGGAACg                                          <  2:607836/140‑1 (MQ=255)
                                                                                     gCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCGGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTCCCACACCACCCGCCAGTGGCAGGAACg                                          <  2:592226/140‑1 (MQ=255)
                                                                                           atgatgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGtaa                                    <  2:542701/140‑1 (MQ=255)
                                                                                                  ccccGTTCGACAGCATAAAATTCTCTGGCAACCACGGCAACAGGACCGAAGTCTCCTATCGGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CAAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTg                             <  2:714320/139‑1 (MQ=255)
                                                                                                   cccGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGtaata                                  <  1:780057/134‑1 (MQ=255)
                                                                                                   cccGTTCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGtaata                                  >  2:780057/1‑134 (MQ=255)
                                                                                                       ttCGACAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGt                        >  1:652615/1‑140 (MQ=255)
                                                                                                       acagACAGCATCAATTTCTCGGCCACCCACGGCAACATGACCGAAGTCTCCTATCGGGATGCGAAACGTGCCGCGAAAAAAGGTG‑CTAAGTATTCCCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGt                        <  2:1100265/137‑1 (MQ=255)
                                                                                                            cAGCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAgcg                   >  2:160402/1‑140 (MQ=255)
                                                                                                              gCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCa                 >  1:1085316/1‑140 (MQ=255)
                                                                                                              gCATCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGGGCCGCGAAGAAAGGTG‑CTAAGTATTCCCACACCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCa                 <  2:23471/140‑1 (MQ=255)
                                                                                                                 tCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCa                 <  1:1164909/137‑1 (MQ=255)
                                                                                                                 tCAAATTCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCa                 >  2:1164909/1‑137 (MQ=255)
                                                                                                                      ttCTCTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATcctgt         >  1:1100265/1‑136 (MQ=255)
                                                                                                                       tctcTGGCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGtat        >  1:303701/1‑140 (MQ=255)
                                                                                                                        ttcaggCAACCAGGGCAACATGCCAGAAGTCTCATGTCAGGTTGGGAAA‑GTTCCGGAAAAAAAGGGGTCAAAATATTCCCAAATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGtata       <  2:1025496/136‑1 (MQ=255)
                                                                                                                             gCAACCACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTataaataa  <  1:785038/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
CTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTG‑CTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTCAGCGCAGATCTGTATAAATAA  >  NC_000913/1679107‑1679371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A