Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,690,705	T→C	V285A (GTA→GCA)	ydgA →	conserved protein YdgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,690,705	0	T	C	100.0%	175.0 / NA	49	V285A (GTA→GCA)	ydgA	conserved protein YdgA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/23); total (26/23)

GAAAAAATGACCATTTCAGTGGAAGGCAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACA  >  NC_000913/1690569‑1690839

gAAAAAATGACCATTTCAGTGGAAGGCAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGcaca                                                                                                                                     <  1:87237/140‑1 (MQ=255)

gAAAAAATGACCATTTCAGTGGAAGGCAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGcaca                                                                                                                                     <  1:212965/140‑1 (MQ=255)

gAAAAAATGACCATTTCAGTGGAAGGCAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGcaca                                                                                                                                     <  1:248963/140‑1 (MQ=255)

   aaaaTGACCATTTCAGTGGAAGGCAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGaa                                                                                                                                  <  2:35287/140‑1 (MQ=255)

     aaTGACCATTTCAGTGGAAGGCAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATc                                                                                                                                <  2:78658/140‑1 (MQ=255)

                        ggCAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCaa                                                                                                             >  1:841635/1‑140 (MQ=255)

                        ggCAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCaa                                                                                                             >  1:647963/1‑140 (MQ=255)

                        ggCAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCaa                                                                                                             >  2:1193535/1‑140 (MQ=255)

                          cAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGc                                                                                                           >  2:497351/1‑140 (MQ=255)

                                          ctgGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCgg                                                                                           >  1:333477/1‑140 (MQ=255)

                                                  cATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTg                                                                                   >  2:542935/1‑140 (MQ=255)

                                                    tGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGc                                                                                          >  2:1087251/1‑131 (MQ=255)

                                                    tGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGc                                                                                          <  1:1087251/131‑1 (MQ=255)

                                                    tGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGAt                                                                                 >  1:8830/1‑140 (MQ=255)

                                                    tGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGAt                                                                                 >  2:882767/1‑140 (MQ=255)

                                                       agaTCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGt                                                                              <  2:1115866/140‑1 (MQ=255)

                                                       agaTCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGt                                                                              <  1:542935/140‑1 (MQ=255)

                                                        gaTCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTg                                                                             <  2:783544/140‑1 (MQ=255)

                                                            aGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGc                                                                         >  1:90874/1‑140 (MQ=255)

                                                               ggTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTg                                                                      >  2:1230377/1‑140 (MQ=255)

                                                                  aaaTCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCa                                                                   <  1:1230377/140‑1 (MQ=255)

                                                                  aaaTCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCa                                                                   <  1:195956/140‑1 (MQ=255)

                                                                  aaaTCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCa                                                                   <  1:714529/140‑1 (MQ=255)

                                                                        gATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGtt                                                             >  1:1012202/1‑140 (MQ=255)

                                                                          tCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTa                                                           <  2:980708/140‑1 (MQ=255)

                                                                          tCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTa                                                           <  2:90874/140‑1 (MQ=255)

                                                                            tGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGc                                                         <  2:8830/140‑1 (MQ=255)

                                                                                   tGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAat                                                  >  2:1253556/1‑140 (MQ=255)

                                                                                        gcaaaaCGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGc                                                         >  2:464853/3‑128 (MQ=255)

                                                                                        gTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTa                                                           <  2:210573/126‑1 (MQ=255)

                                                                                        gTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTa                                                           >  1:210573/1‑126 (MQ=255)

                                                                                        gTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGc                                                         <  1:464853/128‑1 (MQ=255)

                                                                                        gTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAAc                                             >  2:503972/1‑140 (MQ=255)

                                                                                            aaCGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACgcgc                                         <  1:503972/140‑1 (MQ=255)

                                                                                                  cAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTc                                   <  2:139795/140‑1 (MQ=255)

                                                                                                     tAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAgg                                <  2:1012202/140‑1 (MQ=255)

                                                                                                      aGCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGgc                               <  1:1193535/140‑1 (MQ=255)

                                                                                                       gCCAACTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGgcg                              >  2:812097/1‑140 (MQ=255)

                                                                                                          aaCTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCgctg                           <  1:882767/140‑1 (MQ=255)

                                                                                                           aCTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCa                                        >  1:432067/1‑126 (MQ=255)

                                                                                                           aCTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCa                                        <  2:432067/126‑1 (MQ=255)

                                                                                                           aCTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGgcgc                             <  2:504673/137‑1 (MQ=255)

                                                                                                           aCTGGATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGgcgc                             >  1:504673/1‑137 (MQ=255)

                                                                                                               gATTACTCGCTAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGc                      <  1:812097/140‑1 (MQ=255)

                                                                                                                         tAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGaa            >  1:798574/1‑140 (MQ=255)

                                                                                                                         tAAACAGCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGaa            >  1:709570/1‑140 (MQ=255)

                                                                                                                               gCCTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGAAATTGcc      >  2:253358/1‑140 (MQ=255)

                                                                                                                                 cTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATGACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGAAATTGCcaa    >  2:347243/1‑140 (MQ=255)

                                                                                                                                 cTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGAAATTGCcaa    <  1:253358/140‑1 (MQ=255)

                                                                                                                                 cTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGAAATTGCcaa    >  1:977071/1‑140 (MQ=255)

                                                                                                                                 cTGAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATCGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGAAATTGCcaa    >  1:28859/1‑140 (MQ=255)

                                                                                                                                   gAAGGCACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGAAATTGCcaaca  >  1:508513/1‑140 (MQ=255)

GAAAAAATGACCATTTCAGTGGAAGGCAAAGAACTGGCACTGCTGGAAGGCATGGAGATCAGCGGTAAATCGGATCTGGTCAATGACGGTAAAACGATCAATAGCCAACTGGATTACTCGCTAAACAGCCTGAAGGTACAGAATCAGGATCTGGGCAGCGGCAAGCTGACTTTAAAAGTCGGCCAGATTGATGGTGAAGCCTGGCATCAGTTTAGCCAGCAATATAACGCGCAAACTCAGGCGCTGCTGGCACAGCCAGAAATTGCCAACA  >  NC_000913/1690569‑1690839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A