Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,843,762 A→G intergenic (+48/+69) gdhA → / ← ynjI glutamate dehydrogenase/DUF1266 domain‑containing protein YnjI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,843,7620AG98.2% 189.4 / ‑4.5 55intergenic (+48/+69)gdhA/ynjIglutamate dehydrogenase/DUF1266 domain‑containing protein YnjI
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (28/26);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCACAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGCATTTCTTACTG  >  NC_000913/1843629‑1843897
                                                                                                                                     |                                                                                                                                       
gTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTc                                                                                                                                   >  1:982438/1‑140 (MQ=255)
gTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTc                                                                                                                                   >  2:179802/1‑140 (MQ=255)
  gAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCAt                                                                                                                                 <  1:762939/140‑1 (MQ=255)
   aGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCAtt                                                                                                                                >  2:746530/1‑140 (MQ=255)
   aGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCAtt                                                                                                                                >  1:552494/1‑140 (MQ=255)
    gCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTg                                                                                                                               >  1:771511/1‑140 (MQ=255)
         ccAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGtt                                                                                                                          <  1:92974/140‑1 (MQ=255)
          cAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTa                                                                                                                         <  2:982438/140‑1 (MQ=255)
          cAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTa                                                                                                                         <  1:687595/140‑1 (MQ=255)
          cAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTa                                                                                                                         <  2:771511/140‑1 (MQ=255)
          cAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTa                                                                                                                         <  1:1146179/140‑1 (MQ=255)
            aCTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACg                                                                                                                       <  1:1209084/140‑1 (MQ=255)
              tACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGct                                                                                                                     <  2:135887/140‑1 (MQ=255)
              tACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGct                                                                                                                     <  2:1072828/140‑1 (MQ=255)
              tACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGct                                                                                                                     <  2:969496/140‑1 (MQ=255)
                       ggCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAg                                                                                                            >  1:133372/1‑140 (MQ=255)
                        gcgcGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTa                                                                                                                         <  2:56920/126‑1 (MQ=255)
                        gcgcGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTa                                                                                                                         >  2:660727/1‑126 (MQ=255)
                        gcgcGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTa                                                                                                                         >  1:56920/1‑126 (MQ=255)
                        gcgcGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTa                                                                                                                         <  1:660727/126‑1 (MQ=255)
                           cgAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCg                                                                                                        >  2:418411/1‑140 (MQ=255)
                                                    tGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCaaaa                                                                               >  2:461529/1‑140 (MQ=255)
                                                     gCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAAt                                                                              >  2:1062569/1‑140 (MQ=255)
                                                     gCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAAt                                                                              >  1:809912/1‑140 (MQ=255)
                                                        gatgcgatgcTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTc                                                                           <  1:635313/140‑1 (MQ=255)
                                                               tgcTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGtt                                                                    <  2:67119/140‑1 (MQ=255)
                                                                     cgcAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAg                                                              <  1:1062569/140‑1 (MQ=255)
                                                                     cgcAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAg                                                              <  2:153724/140‑1 (MQ=255)
                                                                               gATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACgg                                                    >  1:516357/1‑140 (MQ=255)
                                                                                    aaGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCTGGCAAAATTTCAGGCGTTTATGAGTATTTAACGgatgat                                               >  1:235316/1‑140 (MQ=255)
                                                                                    aaGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGgatgat                                               <  1:771925/140‑1 (MQ=255)
                                                                                    aaGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGgatgat                                               >  2:771925/1‑140 (MQ=255)
                                                                                    aaGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGgatgat                                               >  2:334310/1‑140 (MQ=255)
                                                                                      gTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCG‑CCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCt                                            >  1:839107/1‑140 (MQ=255)
                                                                                      gTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCACAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGc                                             >  1:145382/1‑140 (MQ=255)
                                                                                          tAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTccc                                         >  1:1173306/1‑140 (MQ=255)
                                                                                           aaaTGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTcccc                                        >  1:911046/1‑140 (MQ=255)
                                                                                            aaTGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCa                                       <  2:552494/140‑1 (MQ=255)
                                                                                                ccTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGa                                   <  1:746530/140‑1 (MQ=255)
                                                                                                ccTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGa                                   <  1:461529/140‑1 (MQ=255)
                                                                                                        cgcTACGCTTATCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCt                           >  2:388693/1‑140 (MQ=255)
                                                                                                                  aTCAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCaa                 >  1:727890/1‑140 (MQ=255)
                                                                                                                    cAGGCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACg               <  2:516357/140‑1 (MQ=255)
                                                                                                                      ggCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGc             <  2:180021/140‑1 (MQ=255)
                                                                                                                      ggCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGc             <  1:1041500/140‑1 (MQ=255)
                                                                                                                      ggCCTACAAATGGGCACAATTCATTGCAGTTTCGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCGCCACGTAACATTTCTTATCGGCCACCggt             <  2:145382/140‑2 (MQ=255)
                                                                                                                       gCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGCa            <  2:727890/140‑1 (MQ=255)
                                                                                                                       gCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGCa            >  1:1236896/1‑140 (MQ=255)
                                                                                                                       gCCTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGCa            <  2:911046/140‑1 (MQ=255)
                                                                                                                        ccTACAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGCAt           >  2:172859/1‑140 (MQ=255)
                                                                                                                            cAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGCATTTCt       >  2:622281/1‑140 (MQ=255)
                                                                                                                            cAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGCATTTCt       >  2:583781/1‑140 (MQ=255)
                                                                                                                            cAAATGGGCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGCATTTCt       >  2:905210/1‑140 (MQ=255)
                                                                                                                                 gggCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGc             <  2:614540/129‑1 (MQ=255)
                                                                                                                                 gggCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGc             >  1:614540/1‑129 (MQ=255)
                                                                                                                                 gggCGCAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGCATTTCTTACTg  >  1:261787/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GTGAGCAAACCAACTACGTGCAGGGCGCGAACATTGCCGGTTTTGTGAAGGTTGCCGATGCGATGCTGGCGCAGGGTGTGATTTAAGTTGTAAATGCCTGATGGCGCTACGCTTATCAGGCCTACAAATGGGCACAATTCATTGCAGTTACGCTCTAATGTAGGCCGGGCAAGCGCAGCGCCCCCGGCAAAATTTCAGGCGTTTATGAGTATTTAACGGATGATGCTCCCCACGGAACATTTCTTATGGGCCAACGGCATTTCTTACTG  >  NC_000913/1843629‑1843897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A