Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F28 I1 R1 260 73.5 2514320 99.3% 2496719 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,920,932 G→A R237H (CGT→CAT)  rsmF → 16S rRNA m(5)C1407 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,920,9320GA100.0% 205.1 / NA 58R237H (CGT→CAT) rsmF16S rRNA m(5)C1407 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/32);  total (26/32)

TTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCAAAC  >  NC_000913/1920796‑1921068
                                                                                                                                        |                                                                                                                                        
ttGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATcc                                                                                                                                       <  2:142380/140‑1 (MQ=255)
 tGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCt                                                                                                                                      <  2:693903/140‑1 (MQ=255)
   cTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCtgg                                                                                                                                    <  1:447002/140‑1 (MQ=255)
     ggACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCtggtg                                                                                                                                  >  2:136764/1‑140 (MQ=255)
        cGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTa                                                                                                                               >  2:969574/1‑140 (MQ=255)
        cGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTa                                                                                                                               >  2:81049/1‑140 (MQ=255)
           tCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACg                                                                                                                             >  2:583989/1‑139 (MQ=255)
           tCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACg                                                                                                                             <  1:583989/139‑1 (MQ=255)
              cTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTgg                                                                                                                         <  2:476539/140‑1 (MQ=255)
                    tGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACt                                                                                                                   >  2:1010133/1‑140 (MQ=255)
                    tGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACt                                                                                                                   >  1:683229/1‑140 (MQ=255)
                      gCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCg                                                                                                                 >  2:93535/1‑140 (MQ=255)
                        gAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGAc                                                                                                               <  2:458220/140‑1 (MQ=255)
                         aaGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGAcc                                                                                                              >  2:318972/1‑140 (MQ=255)
                          aGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCt                                                                                                             <  1:320366/140‑1 (MQ=255)
                           ggCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTg                                                                                                            <  1:81049/140‑1 (MQ=255)
                                ggtgCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCt                                                                                                       >  1:381672/1‑140 (MQ=255)
                                ggtgCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCt                                                                                                       >  1:278470/1‑140 (MQ=255)
                                 gtgCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCtt                                                                                                      <  2:82439/140‑1 (MQ=255)
                                      tAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAAcc                                                                                                 >  1:405024/1‑140 (MQ=255)
                                         aGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAgg                                                                                              <  1:1010133/140‑1 (MQ=255)
                                            tCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGgaag                                                                                           >  2:358349/1‑140 (MQ=255)
                                            tCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGgaag                                                                                           <  2:377361/140‑1 (MQ=255)
                                                 aTGCGCTAAAAAACTGGTCACTAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGTCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAAc                                                                                      <  2:1141313/140‑1 (MQ=255)
                                                   gcgcTAAAAAACTGGTCTCCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGa                                                                                    <  1:1207105/140‑1 (MQ=255)
                                                   gcgcTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGa                                                                                    <  1:318972/140‑1 (MQ=255)
                                                   gcgcTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGa                                                                                    <  1:1122110/140‑1 (MQ=255)
                                                     gcTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAg                                                                                  >  2:714971/1‑140 (MQ=255)
                                                     gcTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAg                                                                                  <  1:969574/140‑1 (MQ=255)
                                                             aCTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGc                                                                          <  2:381672/140‑1 (MQ=255)
                                                               tGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCAGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCt                                                                        <  1:93535/140‑1 (MQ=255)
                                                                 gTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCtgt                                                                      >  2:400632/1‑140 (MQ=255)
                                                                   cACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTgg                                                                    <  2:683229/140‑1 (MQ=255)
                                                                      cAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTg                                                                 >  1:205162/1‑140 (MQ=255)
                                                                             cAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAgaga                                                          >  2:52163/1‑140 (MQ=255)
                                                                                   ggAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTAcc                                                    >  2:693481/1‑140 (MQ=255)
                                                                                      aaTCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCg                                                 <  1:400632/140‑1 (MQ=255)
                                                                                       aTCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCg                                                 >  1:1079276/1‑139 (MQ=255)
                                                                                       aTCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCg                                                 <  2:1079276/139‑1 (MQ=255)
                                                                                       aTCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGa                                                <  2:278470/140‑1 (MQ=255)
                                                                                                acacAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTaga                                       <  2:205162/140‑1 (MQ=255)
                                                                                                acacAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTaga                                       <  2:1148240/140‑1 (MQ=255)
                                                                                                        ggAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTAc                               <  1:112510/140‑1 (MQ=255)
                                                                                                        ggAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTAc                               >  1:553282/1‑140 (MQ=255)
                                                                                                        ggAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTAc                               >  2:112510/1‑140 (MQ=255)
                                                                                                         gAGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTAcc                              >  2:136936/1‑140 (MQ=255)
                                                                                                          aGCTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCa                             >  2:838979/1‑140 (MQ=255)
                                                                                                            cTTATCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACt                           >  1:41338/1‑140 (MQ=255)
                                                                                                               aTCGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTgg                        <  2:41338/140‑1 (MQ=255)
                                                                                                                 cGACAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCg                      <  1:358349/140‑1 (MQ=255)
                                                                                                                   aCAGCGCCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGAt                    <  1:714971/140‑1 (MQ=255)
                                                                                                                       cgcCTTTCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATctct                >  1:857903/1‑140 (MQ=255)
                                                                                                                            ttCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCt           <  1:838979/140‑1 (MQ=255)
                                                                                                                            ttCATGCATTACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCt           <  2:382723/140‑1 (MQ=255)
                                                                                                                                    ttACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCt           <  2:477060/132‑1 (MQ=255)
                                                                                                                                    ttACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCt           >  1:477060/1‑132 (MQ=255)
                                                                                                                                    ttACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCAGTTTGCCTGTGGCTGAAAGAGACTTACCCAGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGcaaa   >  1:164030/1‑140 (MQ=255)
                                                                                                                                     tACATCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGcaaac  <  1:136764/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGACGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCAAAC  >  NC_000913/1920796‑1921068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A