Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	260	73.5	2514320	99.3%	2496719	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,012,563	(G)_6→7	pseudogene (62/279 nt)	intG →	putative defective phage integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,012,557	1	.	G	98.9%	311.0 / ‑3.5	87	pseudogene (56/279 nt)	intG	putative defective phage integrase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (39/47); total (40/47)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 102 total aligned reads displayed.

AAAAATCGCTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGT  >  NC_000913/2012420‑2012691

aaaaaTCGCTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑g                                                                                                                                      <  1:51400/140‑1 (MQ=255)

aaaaaTCGCTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑g                                                                                                                                      <  1:808421/140‑1 (MQ=255)

aaaaaTCGCTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑g                                                                                                                                      <  2:177114/140‑1 (MQ=255)

aaaaaTCGCTCATATCCATCCCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑g                                                                                                                                      <  2:523605/140‑1 (MQ=255)

     tcacTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑gggggg                                                                                                                                 >  1:389224/4‑139 (MQ=255)

     tCGCTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑gggggg                                                                                                                                 <  2:629358/140‑2 (MQ=255)

     tCGCTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑gggggg                                                                                                                                 <  1:761480/140‑2 (MQ=255)

      cGCTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑ggggggt                                                                                                                                >  1:906486/1‑138 (MQ=255)

       gCTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑ggggggtt                                                                                                                               <  2:906486/140‑4 (MQ=255)

       gCTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑ggggggtt                                                                                                                               >  2:1081473/1‑137 (MQ=255)

        cTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑ggggggtta                                                                                                                              >  1:469058/1‑136 (MQ=255)

        cTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑ggggggtta                                                                                                                              >  1:924942/1‑136 (MQ=255)

        cTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑ggggggtta                                                                                                                              >  1:183613/1‑136 (MQ=255)

        cTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑ggggggtta                                                                                                                              >  2:261644/1‑136 (MQ=255)

         tCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑g‑ggggggttat                                                                                                                             <  2:389224/140‑6 (MQ=255)

             atCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATcgcg                                                                                                                          >  1:345124/1‑140 (MQ=255)

               ccATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGta                                                                                                                        <  2:469058/140‑1 (MQ=255)

                   cGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACg                                                                                                                    >  2:593951/1‑140 (MQ=255)

                     cccGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGa                                                                                                                  <  2:277371/140‑1 (MQ=255)

                     cccGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGa                                                                                                                  >  1:580802/1‑140 (MQ=255)

                       cGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGCCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATc                                                                                                                >  2:562047/1‑140 (MQ=255)

                          aaTATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTg                                                                                                             <  2:156592/140‑1 (MQ=255)

                          aaTATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTg                                                                                                             <  2:295220/140‑1 (MQ=255)

                            tatGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGa                                                                                                           <  2:699865/140‑1 (MQ=255)

                            tatGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGa                                                                                                           <  1:454853/140‑1 (MQ=255)

                             atGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGAt                                                                                                          <  1:1040835/140‑1 (MQ=255)

                             atGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGAt                                                                                                          <  1:467450/140‑1 (MQ=255)

                                    aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAGGG‑GGGGGTTATCGCGtata                                                                                                                      <  1:980381/122‑1 (MQ=255)

                                    aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa                                                                                                   >  2:135364/1‑140 (MQ=255)

                                    aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa                                                                                                   >  1:497218/1‑140 (MQ=255)

                                    aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa                                                                                                   >  2:744324/1‑140 (MQ=255)

                                    aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa                                                                                                   >  2:586221/1‑140 (MQ=255)

                                    aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa                                                                                                   >  1:1165942/1‑140 (MQ=255)

                                    aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa                                                                                                   >  2:466391/1‑140 (MQ=255)

                                    aaaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGaa                                                                                                   >  1:1199046/1‑140 (MQ=255)

                                     aaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGaaa                                                                                                  <  1:586221/140‑1 (MQ=255)

                                     aaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGaaa                                                                                                  <  1:593951/140‑1 (MQ=255)

                                     aaaCATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGaaa                                                                                                  <  2:183613/140‑1 (MQ=255)

                                          tCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTTTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGtt                                                                                             >  2:1093471/1‑140 (MQ=255)

                                          tCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGtt                                                                                             <  1:164686/140‑1 (MQ=255)

                                          tCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGtt                                                                                             <  1:261644/140‑1 (MQ=255)

                                              caaAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAgg                                                                                         <  1:744324/140‑1 (MQ=255)

                                                   aTTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAg                                                                                    <  2:345124/140‑1 (MQ=255)

                                                   aTTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAg                                                                                    >  1:430357/1‑140 (MQ=255)

                                                   aTTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAg                                                                                    <  2:924942/140‑1 (MQ=255)

                                                       aaTTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTAt                                                                                <  2:841990/140‑1 (MQ=255)

                                                            tCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATc                                                                               >  1:590793/1‑136 (MQ=255)

                                                            tCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATc                                                                               <  2:590793/136‑1 (MQ=255)

                                                              cAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTAAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCAtttt                                                                         <  1:53455/140‑1 (MQ=255)

                                                                 agGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATAGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTaaa                                                                      >  2:731569/1‑140 (MQ=255)

                                                                  ggTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAAc                                                                     >  2:665551/1‑140 (MQ=255)

                                                                   gTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAAcc                                                                    >  1:769684/1‑140 (MQ=255)

                                                                      aCTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTAt                                                                                <  1:978791/125‑1 (MQ=255)

                                                                      aCTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTAt                                                                                >  2:978791/1‑125 (MQ=255)

                                                                             cGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTaa                                                                       >  2:623029/1‑127 (MQ=255)

                                                                             cGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTaa                                                                       <  1:623029/127‑1 (MQ=255)

                                                                             cGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATtgag                                                          >  2:762886/1‑140 (MQ=255)

                                                                             cGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATtgag                                                          >  1:503923/1‑140 (MQ=255)

                                                                             cGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATAAGACGCTCTGATGTTCA‑‑GAGGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATATTAAACCAGAAATtgag                                                          >  2:1118056/1‑140 (MQ=255)

                                                                               tCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCAtttt                                                                         <  2:437560/123‑1 (MQ=255)

                                                                               tCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCAtttt                                                                         >  1:437560/1‑123 (MQ=255)

                                                                               tCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATtgagtg                                                        >  1:102563/1‑140 (MQ=255)

                                                                               tCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATtgagtg                                                        >  2:1222803/1‑140 (MQ=255)

                                                                                  gtgGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTgagtgagt                                                     >  1:1235321/1‑140 (MQ=255)

                                                                                  gtgGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTgagtgagt                                                     <  2:432359/140‑1 (MQ=255)

                                                                                   tgctCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGtt                                                    <  2:769684/137‑1 (MQ=255)

                                                                                        gTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGccc                                               <  2:1162006/140‑1 (MQ=255)

                                                                                            caacaTCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCA‑TTTAAACCAGAAATTGAGTGAGTTTGCCccatcc                                          <  1:1215244/140‑1 (MQ=255)

                                                                                             aacaTCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCccatcc                                          <  1:415576/140‑1 (MQ=255)

                                                                                                   cGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGccc                                    <  2:497218/140‑1 (MQ=255)

                                                                                                    gACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGcccc                                   >  1:803916/1‑140 (MQ=255)

                                                                                                     aCGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAATGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCa                                  <  1:1081473/140‑1 (MQ=255)

                                                                                                     aCGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCa                                  <  2:102563/140‑1 (MQ=255)

                                                                                                     aCGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGGGAGTTTGCCCCATCCATGCCCCa                                  >  2:818315/1‑140 (MQ=255)

                                                                                                      cGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCAc                                 >  2:885478/1‑140 (MQ=255)

                                                                                                          gtgATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCgg                             >  2:151530/1‑140 (MQ=255)

                                                                                                           tgATGTGTCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGt                            <  1:665551/140‑1 (MQ=255)

                                                                                                                tgtCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGAt                       >  1:1044462/1‑140 (MQ=255)

                                                                                                                tgtCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGAt                       <  1:1093471/140‑1 (MQ=255)

                                                                                                                tgtCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGAt                       <  2:1213581/140‑1 (MQ=255)

                                                                                                                 gtCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATc                      >  2:682657/1‑140 (MQ=255)

                                                                                                                  tCAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCg                     >  2:331130/1‑140 (MQ=255)

                                                                                                                   cAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCgg                    <  1:1074086/140‑1 (MQ=255)

                                                                                                                   cAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCgg                    <  2:75867/140‑1 (MQ=255)

                                                                                                                   cAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCgg                    <  1:135364/140‑1 (MQ=255)

                                                                                                                   cAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCgg                    <  2:1165942/140‑1 (MQ=255)

                                                                                                                   cAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCgg                    <  1:331130/140‑1 (MQ=255)

                                                                                                                   cAAATGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCgg                    >  1:462341/1‑140 (MQ=255)

                                                                                                                      aTGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATg                 <  1:151530/140‑1 (MQ=255)

                                                                                                                      aTGAGACGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATg                 <  2:1152574/140‑1 (MQ=255)

                                                                                                                        gagaCGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTa               <  2:580802/140‑1 (MQ=255)

                                                                                                                         agaCGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTaa              <  2:1199046/140‑1 (MQ=255)

                                                                                                                          gaCGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAAt             >  2:442778/1‑140 (MQ=255)

                                                                                                                          gaCGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAAt             >  1:631422/1‑140 (MQ=255)

                                                                                                                           aCGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAAtt            >  2:165040/1‑140 (MQ=255)

                                                                                                                            cGCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTa           >  1:1175907/1‑140 (MQ=255)

                                                                                                                             gCTCTGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTaa          <  1:442778/140‑1 (MQ=255)

                                                                                                                               tctGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTaaca        >  1:1225862/1‑140 (MQ=255)

                                                                                                                                ctGATGTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTaacaa       <  1:682657/140‑1 (MQ=255)

                                                                                                                                     gTTCAG‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGt  <  2:503923/140‑1 (MQ=255)

AAAAATCGCTCATATCCATCGCCCGGAATATGAATAAAAACATCAACAAAGATTAAATTATCCAGAGGTTACTGTTGCGTCTGTGGTCGTAACAACATCCGACGTGGTGATGTGTCAAATGAGACGCTCTGATGTTCA‑‑G‑GGGGGTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGT  >  NC_000913/2012420‑2012691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A